Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Basic & Clinical Pharmacology & Toxicology |
| Vol/bind | 98 |
| Udgave nummer | 2 |
| Sider (fra-til) | 142-9 |
| Antal sider | 7 |
| ISSN | 1742-7835 |
| DOI | |
| Status | Udgivet - 2006 |
Bibliografisk note
Keywords: Adult; Aged; Bumetanide; Dioxanes; Duodenum; Female; Helicobacter Infections; Helicobacter pylori; Humans; Intestinal Secretions; Ion Transport; Ketanserin; Male; Middle Aged; Ondansetron; Piperidines; Receptors, Serotonin; Serotonin; Serotonin Antagonists; Sodium Potassium Chloride Symporter InhibitorsAdgang til dokumentet
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I: Basic & Clinical Pharmacology & Toxicology, Bind 98, Nr. 2, 2006, s. 142-9.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
}
TY - JOUR
T1 - Functional characterization of serotonin receptor subtypes in human duodenal secretion.
AU - Engelmann, Bodil Elisabeth
AU - Bindslev, Niels
AU - Poulsen, Steen Seier
AU - Larsen, Rune
AU - Hansen, Mark Berner
N1 - Keywords: Adult; Aged; Bumetanide; Dioxanes; Duodenum; Female; Helicobacter Infections; Helicobacter pylori; Humans; Intestinal Secretions; Ion Transport; Ketanserin; Male; Middle Aged; Ondansetron; Piperidines; Receptors, Serotonin; Serotonin; Serotonin Antagonists; Sodium Potassium Chloride Symporter Inhibitors
PY - 2006
Y1 - 2006
N2 - Serotonin (5-HT) stimulates ion secretion in the gastrointestinal tract and the sensitivity for 5-HT might be altered in dyspeptic patients infected with Helicobacter pylori. The purpose of the present study was to characterize the 5-HT-induced electrogenic ion transport in the duodenum of dyspeptic patients with or without Helicobacter pylori infection, and to determine the 5-HT receptor subtypes functionally involved. Biopsies from the second part of duodenum were obtained from 43 dyspeptic patients during routine endoscopy. Biopsies were mounted in modified Ussing chambers with air suction for measurements of short-circuit current by a previously validated technique. Short-circuit current was measured before and after application of graded cumulative doses of 5-HT and a single dose of bumetanide (an inhibitor of chloride/bicarbonate transport), or one of the selective 5-HT receptor antagonists: ketanserin, ondansetron, or SB-204070 (1-butyl-4 piperidinmethyl-8-amino-7-chloro-2,3-dihydro-1,4-benzodioxin-5-carboxylate HCl). Histological examination was performed on duodenal biopsies. Helicobacter urease testing and histological examination determined Helicobacter pylori infection. 5-HT induced a dose-dependent and bumetanide-sensitive short-circuit current, which was independent of the presence of Helicobacter pylori infection. All the three 5-HT receptor antagonists failed to significantly effect basal and 5-HT-induced short-circuit current. Our results indicate that in human duodenum 1) 5-HT is a potent stimulator of bumetanide-sensitive secretion, 2) the serotonergic receptor subtype, which acts as the main mediator of 5-HT-induced secretion is different from the 5-HT(2), 5-HT(3), and the 5-HT(4) subtype and, 3) the sensitivity to 5-HT is not altered by Helicobacter pylori infection.
AB - Serotonin (5-HT) stimulates ion secretion in the gastrointestinal tract and the sensitivity for 5-HT might be altered in dyspeptic patients infected with Helicobacter pylori. The purpose of the present study was to characterize the 5-HT-induced electrogenic ion transport in the duodenum of dyspeptic patients with or without Helicobacter pylori infection, and to determine the 5-HT receptor subtypes functionally involved. Biopsies from the second part of duodenum were obtained from 43 dyspeptic patients during routine endoscopy. Biopsies were mounted in modified Ussing chambers with air suction for measurements of short-circuit current by a previously validated technique. Short-circuit current was measured before and after application of graded cumulative doses of 5-HT and a single dose of bumetanide (an inhibitor of chloride/bicarbonate transport), or one of the selective 5-HT receptor antagonists: ketanserin, ondansetron, or SB-204070 (1-butyl-4 piperidinmethyl-8-amino-7-chloro-2,3-dihydro-1,4-benzodioxin-5-carboxylate HCl). Histological examination was performed on duodenal biopsies. Helicobacter urease testing and histological examination determined Helicobacter pylori infection. 5-HT induced a dose-dependent and bumetanide-sensitive short-circuit current, which was independent of the presence of Helicobacter pylori infection. All the three 5-HT receptor antagonists failed to significantly effect basal and 5-HT-induced short-circuit current. Our results indicate that in human duodenum 1) 5-HT is a potent stimulator of bumetanide-sensitive secretion, 2) the serotonergic receptor subtype, which acts as the main mediator of 5-HT-induced secretion is different from the 5-HT(2), 5-HT(3), and the 5-HT(4) subtype and, 3) the sensitivity to 5-HT is not altered by Helicobacter pylori infection.
U2 - 10.1111/j.1742-7843.2006.pto_262.x
DO - 10.1111/j.1742-7843.2006.pto_262.x
M3 - Journal article
C2 - 16445586
SN - 1742-7835
VL - 98
SP - 142
EP - 149
JO - Basic & Clinical Pharmacology & Toxicology
JF - Basic & Clinical Pharmacology & Toxicology
IS - 2
ER -