Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

E A Winzeler; D D Shoemaker; A Astromoff; H Liang; K Anderson; B Andre; R Bangham; R Benito; J D Boeke; H Bussey; A M Chu; C Connelly; K Davis; F Dietrich; S W Dow; M El Bakkoury; F Foury; S H Friend; E Gentalen; G Giaever; J H Hegemann; T Jones; M Laub; H Liao; N Liebundguth; D J Lockhart; A Lucau-Danila; M Lussier; N M'Rabet; P Menard; M Mittmann; C Pai; C Rebischung; J L Revuelta; L Riles; C J Roberts; P Ross-MacDonald; B Scherens; M Snyder; S Sookhai-Mahadeo; R K Storms; S Véronneau; M Voet; G Volckaert; T R Ward; R Wysocki; G S Yen; K Yu; K Zimmermann; P Philippsen; M Johnston; R W Davis

Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

E A Winzeler, D D Shoemaker, A Astromoff, H Liang, K Anderson, B Andre, R Bangham, R Benito, J D Boeke, H Bussey, A M Chu, C Connelly, K Davis, F Dietrich, S W Dow, M El Bakkoury, F Foury, S H Friend, E Gentalen, G GiaeverJ H Hegemann, T Jones, M Laub, H Liao, N Liebundguth, D J Lockhart, A Lucau-Danila, M Lussier, N M'Rabet, P Menard, M Mittmann, C Pai, C Rebischung, J L Revuelta, L Riles, C J Roberts, P Ross-MacDonald, B Scherens, M Snyder, S Sookhai-Mahadeo, R K Storms, S Véronneau, M Voet, G Volckaert, T R Ward, R Wysocki, G S Yen, K Yu, K Zimmermann, P Philippsen, M Johnston, R W Davis

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

3449 Citationer (Scopus)

Abstract

Udgivelsesdato: 1999-Aug-6

Originalsprog	Engelsk
Tidsskrift	Science
Vol/bind	285
Udgave nummer	5429
Sider (fra-til)	901-6
Antal sider	5
ISSN	0036-8075
Status	Udgivet - 1999
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Culture Media; Gene Deletion; Gene Expression Regulation, Fungal; Gene Targeting; Genes, Essential; Genes, Fungal; Genome, Fungal; Open Reading Frames; Phenotype; Polymerase Chain Reaction; Recombination, Genetic; Saccharomyces cerevisiae

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Citationsformater

Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., Chu, A. M., Connelly, C., Davis, K., Dietrich, F., Dow, S. W., El Bakkoury, M., Foury, F., Friend, S. H., Gentalen, E., ... Davis, R. W. (1999). Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science, 285(5429), 901-6.

Winzeler, EA, Shoemaker, DD, Astromoff, A, Liang, H, Anderson, K, Andre, B, Bangham, R, Benito, R, Boeke, JD, Bussey, H, Chu, AM, Connelly, C, Davis, K, Dietrich, F, Dow, SW, El Bakkoury, M, Foury, F, Friend, SH, Gentalen, E, Giaever, G, Hegemann, JH, Jones, T, Laub, M, Liao, H, Liebundguth, N, Lockhart, DJ, Lucau-Danila, A, Lussier, M, M'Rabet, N, Menard, P, Mittmann, M, Pai, C, Rebischung, C, Revuelta, JL, Riles, L, Roberts, CJ, Ross-MacDonald, P, Scherens, B, Snyder, M, Sookhai-Mahadeo, S, Storms, RK, Véronneau, S, Voet, M, Volckaert, G, Ward, TR, Wysocki, R, Yen, GS, Yu, K, Zimmermann, K, Philippsen, P, Johnston, M & Davis, RW 1999, 'Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis', Science, bind 285, nr. 5429, s. 901-6.

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title = "Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis",

abstract = "The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.",

author = "Winzeler, {E A} and Shoemaker, {D D} and A Astromoff and H Liang and K Anderson and B Andre and R Bangham and R Benito and Boeke, {J D} and H Bussey and Chu, {A M} and C Connelly and K Davis and F Dietrich and Dow, {S W} and {El Bakkoury}, M and F Foury and Friend, {S H} and E Gentalen and G Giaever and Hegemann, {J H} and T Jones and M Laub and H Liao and N Liebundguth and Lockhart, {D J} and A Lucau-Danila and M Lussier and N M'Rabet and P Menard and M Mittmann and C Pai and C Rebischung and Revuelta, {J L} and L Riles and Roberts, {C J} and P Ross-MacDonald and B Scherens and M Snyder and S Sookhai-Mahadeo and Storms, {R K} and S V{\'e}ronneau and M Voet and G Volckaert and Ward, {T R} and R Wysocki and Yen, {G S} and K Yu and K Zimmermann and P Philippsen and M Johnston and Davis, {R W}",

note = "Keywords: Culture Media; Gene Deletion; Gene Expression Regulation, Fungal; Gene Targeting; Genes, Essential; Genes, Fungal; Genome, Fungal; Open Reading Frames; Phenotype; Polymerase Chain Reaction; Recombination, Genetic; Saccharomyces cerevisiae",

year = "1999",

language = "English",

volume = "285",

pages = "901--6",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5429",

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TY - JOUR

T1 - Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis

AU - Winzeler, E A

AU - Shoemaker, D D

AU - Astromoff, A

AU - Liang, H

AU - Anderson, K

AU - Andre, B

AU - Bangham, R

AU - Benito, R

AU - Boeke, J D

AU - Bussey, H

AU - Chu, A M

AU - Connelly, C

AU - Davis, K

AU - Dietrich, F

AU - Dow, S W

AU - El Bakkoury, M

AU - Foury, F

AU - Friend, S H

AU - Gentalen, E

AU - Giaever, G

AU - Hegemann, J H

AU - Jones, T

AU - Laub, M

AU - Liao, H

AU - Liebundguth, N

AU - Lockhart, D J

AU - Lucau-Danila, A

AU - Lussier, M

AU - M'Rabet, N

AU - Menard, P

AU - Mittmann, M

AU - Pai, C

AU - Rebischung, C

AU - Revuelta, J L

AU - Riles, L

AU - Roberts, C J

AU - Ross-MacDonald, P

AU - Scherens, B

AU - Snyder, M

AU - Sookhai-Mahadeo, S

AU - Storms, R K

AU - Véronneau, S

AU - Voet, M

AU - Volckaert, G

AU - Ward, T R

AU - Wysocki, R

AU - Yen, G S

AU - Yu, K

AU - Zimmermann, K

AU - Philippsen, P

AU - Johnston, M

AU - Davis, R W

N1 - Keywords: Culture Media; Gene Deletion; Gene Expression Regulation, Fungal; Gene Targeting; Genes, Essential; Genes, Fungal; Genome, Fungal; Open Reading Frames; Phenotype; Polymerase Chain Reaction; Recombination, Genetic; Saccharomyces cerevisiae

PY - 1999

Y1 - 1999

N2 - The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

AB - The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

M3 - Journal article

C2 - 10436161

SN - 0036-8075

VL - 285

SP - 901

EP - 906

JO - Science

JF - Science

IS - 5429

ER -