Functional intron+ and intron- rDNA in the same macronucleus of the ciliate Tetrahymena pigmentosa

Henrik Nielsen; J Engberg

Functional intron+ and intron- rDNA in the same macronucleus of the ciliate Tetrahymena pigmentosa

Henrik Nielsen, J Engberg

Institut for Cellulær og Molekylær Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

12 Citationer (Scopus)

Abstract

Udgivelsesdato: 1985-May-24

Originalsprog	Engelsk
Tidsskrift	BBA General Subjects
Vol/bind	825
Udgave nummer	1
Sider (fra-til)	30-8
Antal sider	8
ISSN	0304-4165
Status	Udgivet - 1985

Bibliografisk note

Keywords: Alleles; Animals; Base Sequence; Cell Nucleus; Cloning, Molecular; Conjugation, Genetic; DNA Restriction Enzymes; DNA, Ribosomal; Nucleic Acid Hybridization; Plasmids; RNA, Ribosomal; Tetrahymena; Transcription, Genetic

Citationsformater

@article{03c543c0a68e11debc73000ea68e967b,

title = "Functional intron+ and intron- rDNA in the same macronucleus of the ciliate Tetrahymena pigmentosa",

abstract = "Diallelic clones of Tetrahymena pigmentosa containing equal amounts of intron+ and intron- rDNA in the macronucleus were constructed. The macronucleus of the resulting strains divides amitotically during vegetative growth and the diallelic genotype is therefore unstable. The coexistence of the two alleles was followed in the total culture and in single cells during their vegetative segregation and it was observed that replication was non-preferential with respect to the two alleles. The diallelic clones were also used to demonstrate that intron-containing rDNA was transcribed and the transcript processed in the presence of corresponding intron- rDNA. The results are discussed in the light of the 'non-function' idea for ribosomal RNA introns.",

author = "Henrik Nielsen and J Engberg",

note = "Keywords: Alleles; Animals; Base Sequence; Cell Nucleus; Cloning, Molecular; Conjugation, Genetic; DNA Restriction Enzymes; DNA, Ribosomal; Nucleic Acid Hybridization; Plasmids; RNA, Ribosomal; Tetrahymena; Transcription, Genetic",

year = "1985",

language = "English",

volume = "825",

pages = "30--8",

journal = "BBA General Subjects",

issn = "0304-4165",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Functional intron+ and intron- rDNA in the same macronucleus of the ciliate Tetrahymena pigmentosa

AU - Nielsen, Henrik

AU - Engberg, J

N1 - Keywords: Alleles; Animals; Base Sequence; Cell Nucleus; Cloning, Molecular; Conjugation, Genetic; DNA Restriction Enzymes; DNA, Ribosomal; Nucleic Acid Hybridization; Plasmids; RNA, Ribosomal; Tetrahymena; Transcription, Genetic

PY - 1985

Y1 - 1985

N2 - Diallelic clones of Tetrahymena pigmentosa containing equal amounts of intron+ and intron- rDNA in the macronucleus were constructed. The macronucleus of the resulting strains divides amitotically during vegetative growth and the diallelic genotype is therefore unstable. The coexistence of the two alleles was followed in the total culture and in single cells during their vegetative segregation and it was observed that replication was non-preferential with respect to the two alleles. The diallelic clones were also used to demonstrate that intron-containing rDNA was transcribed and the transcript processed in the presence of corresponding intron- rDNA. The results are discussed in the light of the 'non-function' idea for ribosomal RNA introns.

AB - Diallelic clones of Tetrahymena pigmentosa containing equal amounts of intron+ and intron- rDNA in the macronucleus were constructed. The macronucleus of the resulting strains divides amitotically during vegetative growth and the diallelic genotype is therefore unstable. The coexistence of the two alleles was followed in the total culture and in single cells during their vegetative segregation and it was observed that replication was non-preferential with respect to the two alleles. The diallelic clones were also used to demonstrate that intron-containing rDNA was transcribed and the transcript processed in the presence of corresponding intron- rDNA. The results are discussed in the light of the 'non-function' idea for ribosomal RNA introns.

M3 - Journal article

C2 - 2986696

SN - 0304-4165

VL - 825

SP - 30

EP - 38

JO - BBA General Subjects

JF - BBA General Subjects

IS - 1

ER -