Fusion genetic analysis of jasmonate-signalling mutants in Arabidopsis

Anders Bøgh Jensen; D. Raventos; John Williams Mundy

doi:10.1046/j.0960-7412.2001.01241.x

Fusion genetic analysis of jasmonate-signalling mutants in Arabidopsis

Anders Bøgh Jensen, D. Raventos, John Williams Mundy

Centre m.v.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

71 Citationer (Scopus)

Abstract

Udgivelsesdato: March 2002

Originalsprog	Engelsk
Tidsskrift	Plant Journal
Vol/bind	29
Udgave nummer	5
Sider (fra-til)	595-606
ISSN	0960-7412
DOI	https://doi.org/10.1046/j.0960-7412.2001.01241.x
Status	Udgivet - 2002

Bibliografisk note

KEYWORDS
bio-imaging • kinase • jasmonate • lipoxygenase • luciferase • phosphatase

Adgang til dokumentet

10.1046/j.0960-7412.2001.01241.x

Citationsformater

@article{3f1a021074c511dbbee902004c4f4f50,

title = "Fusion genetic analysis of jasmonate-signalling mutants in Arabidopsis",

abstract = "Jasmonates induce plant-defence responses and act to regulate defence-related genes including positive feedback of the lipoxygenase 2 (LOX2) gene involved in jasmonate synthesis. To identify jasmonate-signalling mutants, we used a fusion genetic strategy in which the firefly luciferase (FLUC) and Escherichia coli{\ss}-glucuronidase (GUS) reporters were expressed under control of the jasmonate-responsive LOX2 promoter. Spatial and temporal patterns of reporter expression were determined initially, and revealed that JA-responsive expression from the LOX2 promoter required de novo protein synthesis. Reporter activity was also induced by the protein kinase inhibitor staurosporine and antagonized by the protein phosphatase inhibitor okadaic acid. FLUC bio-imaging, RNA gel-blot analysis and progeny analyses identified three recessive mutants that underexpress the FLUC reporter, designated jue1, 2 and 3, as well as two recessive mutants, designated joe1 and 2, that overexpress the reporter. Genetic analysis indicated that reporter overexpression in the joe mutants requires COI. joe1 responded to MeJA with increased anthocyanin accumulation, while joe2 responded with decreased root growth inhibition. In addition, reporter induction and endogenous LOX2 expression by staurosporine was absent in joe2.",

author = "Jensen, {Anders B{\o}gh} and D. Raventos and Mundy, {John Williams}",

note = "KEYWORDS bio-imaging • kinase • jasmonate • lipoxygenase • luciferase • phosphatase",

year = "2002",

doi = "10.1046/j.0960-7412.2001.01241.x",

language = "English",

volume = "29",

pages = "595--606",

journal = "Plant Journal",

issn = "0960-7412",

publisher = "Wiley-Blackwell",

number = "5",

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TY - JOUR

T1 - Fusion genetic analysis of jasmonate-signalling mutants in Arabidopsis

AU - Jensen, Anders Bøgh

AU - Raventos, D.

AU - Mundy, John Williams

N1 - KEYWORDS bio-imaging • kinase • jasmonate • lipoxygenase • luciferase • phosphatase

PY - 2002

Y1 - 2002

N2 - Jasmonates induce plant-defence responses and act to regulate defence-related genes including positive feedback of the lipoxygenase 2 (LOX2) gene involved in jasmonate synthesis. To identify jasmonate-signalling mutants, we used a fusion genetic strategy in which the firefly luciferase (FLUC) and Escherichia coliß-glucuronidase (GUS) reporters were expressed under control of the jasmonate-responsive LOX2 promoter. Spatial and temporal patterns of reporter expression were determined initially, and revealed that JA-responsive expression from the LOX2 promoter required de novo protein synthesis. Reporter activity was also induced by the protein kinase inhibitor staurosporine and antagonized by the protein phosphatase inhibitor okadaic acid. FLUC bio-imaging, RNA gel-blot analysis and progeny analyses identified three recessive mutants that underexpress the FLUC reporter, designated jue1, 2 and 3, as well as two recessive mutants, designated joe1 and 2, that overexpress the reporter. Genetic analysis indicated that reporter overexpression in the joe mutants requires COI. joe1 responded to MeJA with increased anthocyanin accumulation, while joe2 responded with decreased root growth inhibition. In addition, reporter induction and endogenous LOX2 expression by staurosporine was absent in joe2.

AB - Jasmonates induce plant-defence responses and act to regulate defence-related genes including positive feedback of the lipoxygenase 2 (LOX2) gene involved in jasmonate synthesis. To identify jasmonate-signalling mutants, we used a fusion genetic strategy in which the firefly luciferase (FLUC) and Escherichia coliß-glucuronidase (GUS) reporters were expressed under control of the jasmonate-responsive LOX2 promoter. Spatial and temporal patterns of reporter expression were determined initially, and revealed that JA-responsive expression from the LOX2 promoter required de novo protein synthesis. Reporter activity was also induced by the protein kinase inhibitor staurosporine and antagonized by the protein phosphatase inhibitor okadaic acid. FLUC bio-imaging, RNA gel-blot analysis and progeny analyses identified three recessive mutants that underexpress the FLUC reporter, designated jue1, 2 and 3, as well as two recessive mutants, designated joe1 and 2, that overexpress the reporter. Genetic analysis indicated that reporter overexpression in the joe mutants requires COI. joe1 responded to MeJA with increased anthocyanin accumulation, while joe2 responded with decreased root growth inhibition. In addition, reporter induction and endogenous LOX2 expression by staurosporine was absent in joe2.

U2 - 10.1046/j.0960-7412.2001.01241.x

DO - 10.1046/j.0960-7412.2001.01241.x

M3 - Journal article

VL - 29

SP - 595

EP - 606

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 5

ER -