Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation

Niels-Ulrik Frigaard; Yumiko Sakuragi; Donald A Bryant

doi:10.1385/1-59259-799-8:325

Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation

Niels-Ulrik Frigaard, Yumiko Sakuragi, Donald A Bryant

Bioinformatik og RNA Biologi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

91 Citationer (Scopus)

Abstract

Udgivelsesdato: 2004

Originalsprog	Engelsk
Tidsskrift	Methods in Molecular Biology
Vol/bind	274
Sider (fra-til)	325-340
Antal sider	15
ISSN	1064-3745
DOI	https://doi.org/10.1385/1-59259-799-8:325
Status	Udgivet - 2004

Bibliografisk note

Keywords: Chlorobium; Cyanobacteria; DNA, Bacterial; Gene Silencing; Mutagenesis; Transformation, Bacterial

Adgang til dokumentet

10.1385/1-59259-799-8:325

Citationsformater

Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation. / Frigaard, Niels-Ulrik; Sakuragi, Yumiko; Bryant, Donald A.
I: Methods in Molecular Biology, Bind 274, 2004, s. 325-340.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{d2725c40962511de8bc9000ea68e967b,

title = "Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation",

abstract = "Inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain reaction (PCR), are also presented. These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR.",

author = "Niels-Ulrik Frigaard and Yumiko Sakuragi and Bryant, {Donald A}",

note = "Keywords: Chlorobium; Cyanobacteria; DNA, Bacterial; Gene Silencing; Mutagenesis; Transformation, Bacterial",

year = "2004",

doi = "10.1385/1-59259-799-8:325",

language = "English",

volume = "274",

pages = "325--340",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press",

}

TY - JOUR

T1 - Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation

AU - Frigaard, Niels-Ulrik

AU - Sakuragi, Yumiko

AU - Bryant, Donald A

N1 - Keywords: Chlorobium; Cyanobacteria; DNA, Bacterial; Gene Silencing; Mutagenesis; Transformation, Bacterial

PY - 2004

Y1 - 2004

N2 - Inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain reaction (PCR), are also presented. These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR.

AB - Inactivation of a chromosomal gene is a useful approach to study the function of the gene in question and can be used to produce a desired phenotype in the organism. This chapter describes how to generate such mutants of the cyanobacterium Synechococcus sp. PCC 7002 and the green sulfur bacterium Chlorobium tepidum by natural transformation with synthetic DNA constructs. Two alternative methods to generate the DNA constructs, both performed entirely in vitro and based on the polymerase chain reaction (PCR), are also presented. These methods are ligation of DNA fragments with T4 DNA ligase, and megaprimer PCR.

U2 - 10.1385/1-59259-799-8:325

DO - 10.1385/1-59259-799-8:325

M3 - Journal article

C2 - 15187290

SN - 1064-3745

VL - 274

SP - 325

EP - 340

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -