Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Biological Chemistry |
| Vol/bind | 282 |
| Udgave nummer | 37 |
| Sider (fra-til) | 27270-6 |
| Antal sider | 6 |
| ISSN | 0021-9258 |
| DOI | |
| Status | Udgivet - 2007 |
| Udgivet eksternt | Ja |
Bibliografisk note
Keywords: Glycogen; Glycosyltransferases; Lipopolysaccharides; Methylglucosides; Multigene Family; Mycobacterium tuberculosis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAdgang til dokumentet
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I: Journal of Biological Chemistry, Bind 282, Nr. 37, 2007, s. 27270-6.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis.
AU - Stadthagen, Gustavo
AU - Sambou, Tounkang
AU - Guerin, Marcelo
AU - Barilone, Nathalie
AU - Boudou, Frédéric
AU - Korduláková, Jana
AU - Charles, Patricia
AU - Alzari, Pedro M
AU - Lemassu, Anne
AU - Daffé, Mamadou
AU - Puzo, Germain
AU - Gicquel, Brigitte
AU - Rivière, Michel
AU - Jackson, Mary
N1 - Keywords: Glycogen; Glycosyltransferases; Lipopolysaccharides; Methylglucosides; Multigene Family; Mycobacterium tuberculosis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
PY - 2007
Y1 - 2007
N2 - Mycobacteria produce two unusual polymethylated polysaccharides, the 6-O-methylglucosyl-containing lipopolysaccharides (MGLP) and the 3-O-methylmannose polysaccharides, which have been shown to regulate fatty acid biosynthesis in vitro. A cluster of genes dedicated to the synthesis of MGLP was identified in Mycobacterium tuberculosis and Mycobacterium smegmatis. Overexpression of the putative glycosyltransferase gene Rv3032 in M. smegmatis greatly stimulated MGLP production, whereas the targeted disruption of Rv3032 in M. tuberculosis and that of the putative methyltransferase gene MSMEG2349 in M. smegmatis resulted in a dramatic reduction in the amounts of MGLP synthesized and in the accumulation of precursors of these molecules. Disruption of Rv3032 also led to a significant decrease in the glycogen content of the tubercle bacillus, indicating that the product of this gene is likely to be involved in the elongation of more than one alpha-(1-->4)-glucan in this bacterium. Results thus suggest that Rv3032 encodes the alpha-(1-->4)-glucosyltransferase responsible for the elongation of MGLP, whereas MSMEG2349 encodes the O-methyltransferase required for the 6-O-methylation of these compounds.
AB - Mycobacteria produce two unusual polymethylated polysaccharides, the 6-O-methylglucosyl-containing lipopolysaccharides (MGLP) and the 3-O-methylmannose polysaccharides, which have been shown to regulate fatty acid biosynthesis in vitro. A cluster of genes dedicated to the synthesis of MGLP was identified in Mycobacterium tuberculosis and Mycobacterium smegmatis. Overexpression of the putative glycosyltransferase gene Rv3032 in M. smegmatis greatly stimulated MGLP production, whereas the targeted disruption of Rv3032 in M. tuberculosis and that of the putative methyltransferase gene MSMEG2349 in M. smegmatis resulted in a dramatic reduction in the amounts of MGLP synthesized and in the accumulation of precursors of these molecules. Disruption of Rv3032 also led to a significant decrease in the glycogen content of the tubercle bacillus, indicating that the product of this gene is likely to be involved in the elongation of more than one alpha-(1-->4)-glucan in this bacterium. Results thus suggest that Rv3032 encodes the alpha-(1-->4)-glucosyltransferase responsible for the elongation of MGLP, whereas MSMEG2349 encodes the O-methyltransferase required for the 6-O-methylation of these compounds.
U2 - 10.1074/jbc.M702676200
DO - 10.1074/jbc.M702676200
M3 - Journal article
C2 - 17640872
SN - 0021-9258
VL - 282
SP - 27270
EP - 27276
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 37
ER -