Genetic influence on disease course and cytokine response in relapsing experimental allergic encephalomyelitis.

P Kjellén; Shohreh Issazadeh-Navikas; T Olsson; R Holmdahl

Genetic influence on disease course and cytokine response in relapsing experimental allergic encephalomyelitis.

P Kjellén, Shohreh Issazadeh-Navikas, T Olsson, R Holmdahl

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

23 Citationer (Scopus)

Abstract

Udgivelsesdato: 1998-Mar

Originalsprog	Engelsk
Tidsskrift	International Immunology
Vol/bind	10
Udgave nummer	3
Sider (fra-til)	333-40
Antal sider	7
ISSN	0953-8178
Status	Udgivet - 1998

Bibliografisk note

Keywords: Amino Acid Sequence; Animals; Cytokines; Encephalomyelitis, Autoimmune, Experimental; Genetic Predisposition to Disease; Molecular Sequence Data; Myelin Basic Proteins; RNA, Messenger; Rats; Rats, Inbred Lew; Spinal Cord

Citationsformater

@article{4c3b6d4057d011dd8d9f000ea68e967b,

title = "Genetic influence on disease course and cytokine response in relapsing experimental allergic encephalomyelitis.",

abstract = "A protracted and relapsing form of experimental allergic encephalomyelitis (EAE) develops in the DA rat after immunization with rat spinal cord homogenate (SCH) emulsified in incomplete Freund's adjuvant (IFA). The genetic influence on this model has been analyzed by immunizing MHC congenic strains on both LEW and DA genetic backgrounds, and recombinant inbred strains between DA and E3 rats. An in situ hybridization assay was used to examine the expression of mRNA for IFN-gamma, IL-4, IL-10 and transforming growth factor (TGF)-beta both in sections of spinal cords and the antigen-induced expression for these cytokines by splenocytes after in vitro stimulation with encephalitogenic MBP peptides. The susceptibility of relapsing EAE after immunization with SCH in IFA in the DA strain, but not the E3 strain, was correlated with a lack of expression for TGF-beta in the spinal cord. The recombinant inbred DXEB rats developed a severe EAE while surprisingly no signs of disease were observed in the DXEA strain, which shares the MHC region with the DXEB strain, after immunization with the MBP 63-87 peptide. Resistance to relapsing EAE in the DXEA strain correlated with increased non-MHC controlled expression for TGF-beta and lack of IFN-gamma in the spinal cord. The same pattern of cytokine expression was seen in splenocytes after stimulation in vitro with the MBP 63-87 peptide. A spreading of the immune response to the MBP 87-110 peptide was seen. Non-MHC genes controlled the quality of this response: splenocytes from MBP 63-87 immunized DXEB rats responded in vitro towards the MBP 87-110 peptide by expressing mRNA for IFN-gamma, IL-10 and IL-4, whereas in the DXEA strain the corresponding response involved IL-4 and TGF-beta. Taken together these data show that non-MHC controlled expression of mRNA for TGF-beta is associated with resistance to EAE.",

author = "P Kjell{\'e}n and Shohreh Issazadeh-Navikas and T Olsson and R Holmdahl",

note = "Keywords: Amino Acid Sequence; Animals; Cytokines; Encephalomyelitis, Autoimmune, Experimental; Genetic Predisposition to Disease; Molecular Sequence Data; Myelin Basic Proteins; RNA, Messenger; Rats; Rats, Inbred Lew; Spinal Cord",

year = "1998",

language = "English",

volume = "10",

pages = "333--40",

journal = "International Immunology",

issn = "0953-8178",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Genetic influence on disease course and cytokine response in relapsing experimental allergic encephalomyelitis.

AU - Kjellén, P

AU - Issazadeh-Navikas, Shohreh

AU - Olsson, T

AU - Holmdahl, R

N1 - Keywords: Amino Acid Sequence; Animals; Cytokines; Encephalomyelitis, Autoimmune, Experimental; Genetic Predisposition to Disease; Molecular Sequence Data; Myelin Basic Proteins; RNA, Messenger; Rats; Rats, Inbred Lew; Spinal Cord

PY - 1998

Y1 - 1998

N2 - A protracted and relapsing form of experimental allergic encephalomyelitis (EAE) develops in the DA rat after immunization with rat spinal cord homogenate (SCH) emulsified in incomplete Freund's adjuvant (IFA). The genetic influence on this model has been analyzed by immunizing MHC congenic strains on both LEW and DA genetic backgrounds, and recombinant inbred strains between DA and E3 rats. An in situ hybridization assay was used to examine the expression of mRNA for IFN-gamma, IL-4, IL-10 and transforming growth factor (TGF)-beta both in sections of spinal cords and the antigen-induced expression for these cytokines by splenocytes after in vitro stimulation with encephalitogenic MBP peptides. The susceptibility of relapsing EAE after immunization with SCH in IFA in the DA strain, but not the E3 strain, was correlated with a lack of expression for TGF-beta in the spinal cord. The recombinant inbred DXEB rats developed a severe EAE while surprisingly no signs of disease were observed in the DXEA strain, which shares the MHC region with the DXEB strain, after immunization with the MBP 63-87 peptide. Resistance to relapsing EAE in the DXEA strain correlated with increased non-MHC controlled expression for TGF-beta and lack of IFN-gamma in the spinal cord. The same pattern of cytokine expression was seen in splenocytes after stimulation in vitro with the MBP 63-87 peptide. A spreading of the immune response to the MBP 87-110 peptide was seen. Non-MHC genes controlled the quality of this response: splenocytes from MBP 63-87 immunized DXEB rats responded in vitro towards the MBP 87-110 peptide by expressing mRNA for IFN-gamma, IL-10 and IL-4, whereas in the DXEA strain the corresponding response involved IL-4 and TGF-beta. Taken together these data show that non-MHC controlled expression of mRNA for TGF-beta is associated with resistance to EAE.

AB - A protracted and relapsing form of experimental allergic encephalomyelitis (EAE) develops in the DA rat after immunization with rat spinal cord homogenate (SCH) emulsified in incomplete Freund's adjuvant (IFA). The genetic influence on this model has been analyzed by immunizing MHC congenic strains on both LEW and DA genetic backgrounds, and recombinant inbred strains between DA and E3 rats. An in situ hybridization assay was used to examine the expression of mRNA for IFN-gamma, IL-4, IL-10 and transforming growth factor (TGF)-beta both in sections of spinal cords and the antigen-induced expression for these cytokines by splenocytes after in vitro stimulation with encephalitogenic MBP peptides. The susceptibility of relapsing EAE after immunization with SCH in IFA in the DA strain, but not the E3 strain, was correlated with a lack of expression for TGF-beta in the spinal cord. The recombinant inbred DXEB rats developed a severe EAE while surprisingly no signs of disease were observed in the DXEA strain, which shares the MHC region with the DXEB strain, after immunization with the MBP 63-87 peptide. Resistance to relapsing EAE in the DXEA strain correlated with increased non-MHC controlled expression for TGF-beta and lack of IFN-gamma in the spinal cord. The same pattern of cytokine expression was seen in splenocytes after stimulation in vitro with the MBP 63-87 peptide. A spreading of the immune response to the MBP 87-110 peptide was seen. Non-MHC genes controlled the quality of this response: splenocytes from MBP 63-87 immunized DXEB rats responded in vitro towards the MBP 87-110 peptide by expressing mRNA for IFN-gamma, IL-10 and IL-4, whereas in the DXEA strain the corresponding response involved IL-4 and TGF-beta. Taken together these data show that non-MHC controlled expression of mRNA for TGF-beta is associated with resistance to EAE.

M3 - Journal article

C2 - 9576621

SN - 0953-8178

VL - 10

SP - 333

EP - 340

JO - International Immunology

JF - International Immunology

IS - 3

ER -