Abstract
The phyllosphere, the aboveground part of a plant, is a harsh environment with diverse abiotic and biotic stresses, including oscillating nutrient availability and temperature as well as exposure to UV radiation. Microbial colonization of this dynamic environment requires specific adaptive traits, including tolerance to fluctuating temperatures, the production of secondary metabolites and pigments to successfully compete with other microorganisms and to withstand abiotic stresses. Here, we isolated 175 yeasts, comprising 15 different genera, from the wheat flag leaf and characterized a selection of these for various adaptive traits such as substrate utilization, tolerance to different temperatures, biofilm formation, and antagonism toward the fungal leaf pathogen Fusarium graminearum. Collectively our results revealed that the wheat flag leaf is a rich resource of taxonomically and phenotypically diverse yeast genera that exhibit various traits that can contribute to survival in the harsh phyllosphere environment.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 908628 |
| Tidsskrift | Frontiers in Plant Science |
| Vol/bind | 13 |
| Antal sider | 13 |
| ISSN | 1664-462X |
| DOI | |
| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:We thank Alex Gobbi (Copenhagen University) for processing the wheat flag leaves. We thank Wilhelm Schäfer (Hamburg University) for providing the Fusarium graminearum strain 8/1. We also thank Joost Willemse (Leiden University) for the assistance with the scanning electron microscopy. This manuscript is publication number 7454 of the Netherlands Institute of Ecology (NIOO-KNAW).
Funding Information:
All authors are supported by the Novo Nordisk Foundation (Grant NNF19SA0059348).
Publisher Copyright:
Copyright © 2022 Gouka, Vogels, Hansen, Raaijmakers and Cordovez.