@article{5b92c770f24711dfb6d2000ea68e967b,
title = "Genome-wide analysis of CDX2 binding in intestinal epithelial cells (Caco-2)",
abstract = "The CDX2 transcription factor is known to play a crucial role in inhibiting proliferation, promoting differentiation and the expression of intestinal specific genes in intestinal cells. The overall effect of CDX2 in intestinal cells has previously been investigated in conditional knock-out mice, revealing a critical role of CDX2 in the formation of the normal intestinal identity. The identification of direct targets of transcription factors is a key problem in the study of gene regulatory networks. The ChIP-seq technique combines chromatin immunoprecipitation (ChIP) with next generation sequencing resulting in a high throughput experimental method of identifying direct targets of specific transcription factors. The method was applied to CDX2, leading to the identification of the direct binding of CDX2 to several known and novel target genes in the intestinal cell. Examination of the transcript levels of selected genes verified the regulatory role of CDX2 binding. The results place CDX2 as a key node in a transcription factor network controlling the proliferation and differentiation of intestinal cells.",
author = "Mette Boyd and Morten Hansen and Jensen, {Tine G K} and Anna Perearnau and Olsen, {Anders K} and Bram, {Lotte L} and Mads Bak and Niels Tommerup and J{\o}rgen Olsen and Troelsen, {Jesper T}",
note = "Keywords: Caco-2 Cells; Cell Differentiation; Chromatin Immunoprecipitation; Computational Biology; Electrophoretic Mobility Shift Assay; Enhancer Elements, Genetic; Epithelial Cells; Genome, Human; Hepatocyte Nuclear Factor 4; High Mobility Group Proteins; Homeodomain Proteins; Humans; Intestines; Membrane Proteins; Metalloendopeptidases; Mutation; Promoter Regions, Genetic; Protein Binding; Repressor Proteins; Reverse Transcriptase Polymerase Chain Reaction; Trans-Activators",
year = "2010",
month = aug,
doi = "10.1074/jbc.M109.089516",
language = "English",
volume = "285",
pages = "25115--25",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "33",
}