Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Environmental and Molecular Mutagenesis |
| Vol/bind | 49 |
| Udgave nummer | 6 |
| Sider (fra-til) | 476-87 |
| Antal sider | 11 |
| ISSN | 0893-6692 |
| DOI | |
| Status | Udgivet - 2008 |
Bibliografisk note
Keywords: Animals; Cell Cycle; Cell Line; Cell Proliferation; Cell Survival; Comet Assay; DNA Damage; DNA Mutational Analysis; Epithelial Cells; Fullerenes; Lung; Mice; Mutagenicity Tests; Nanotubes, Carbon; Reactive Oxygen SpeciesAdgang til dokumentet
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Genotoxicity, cytotoxicity, and reactive oxygen species induced by single-walled carbon nanotubes and C(60) fullerenes in the FE1-Mutatrade markMouse lung epithelial cells. / Jacobsen, Nicklas Raun; Pojana, Giulio; White, Paul; Møller, Peter; Cohn, Corey Alexander; Korsholm, Karen Smith; Vogel, Ulla; Marcomini, Antonio; Loft, Steffen; Wallin, Håkan.
I: Environmental and Molecular Mutagenesis, Bind 49, Nr. 6, 2008, s. 476-87.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Genotoxicity, cytotoxicity, and reactive oxygen species induced by single-walled carbon nanotubes and C(60) fullerenes in the FE1-Mutatrade markMouse lung epithelial cells
AU - Jacobsen, Nicklas Raun
AU - Pojana, Giulio
AU - White, Paul
AU - Møller, Peter
AU - Cohn, Corey Alexander
AU - Korsholm, Karen Smith
AU - Vogel, Ulla
AU - Marcomini, Antonio
AU - Loft, Steffen
AU - Wallin, Håkan
N1 - Keywords: Animals; Cell Cycle; Cell Line; Cell Proliferation; Cell Survival; Comet Assay; DNA Damage; DNA Mutational Analysis; Epithelial Cells; Fullerenes; Lung; Mice; Mutagenicity Tests; Nanotubes, Carbon; Reactive Oxygen Species
PY - 2008
Y1 - 2008
N2 - Viability, cell cycle effects, genotoxicity, reactive oxygen species production, and mutagenicity of C(60) fullerenes (C(60)) and single-walled carbon nanotubes (SWCNT) were assessed in the FE1-Mutatrade markMouse lung epithelial cell line. None of these particles induced cell death within 24 hr at doses between 0 and 200 microg/ml or during long-term subculture exposure (576 hr) at 100 microg/ml, as determined by two different assays. However, cell proliferation was slower with SWCNT exposure and a larger fraction of the cells were in the G1 phase. Exposure to carbon black resulted in the greatest reactive oxygen species generation followed by SWCNT and C(60) in both cellular and cell-free particle suspensions. C(60) and SWCNT did not increase the level of strand breaks, but significantly increased the level of FPG sensitive sites/oxidized purines (22 and 56%, respectively) determined by the comet assay. The mutant frequency in the cII gene was unaffected by 576 hr of exposure to either 100 microg/ml C(60) or SWCNT when compared with control incubations, whereas we have previously reported that carbon black and diesel exhaust particles induce mutations using an identical exposure scenario. These results indicate that SWCNT and C(60) are less genotoxic in vitro than carbon black and diesel exhaust particles.
AB - Viability, cell cycle effects, genotoxicity, reactive oxygen species production, and mutagenicity of C(60) fullerenes (C(60)) and single-walled carbon nanotubes (SWCNT) were assessed in the FE1-Mutatrade markMouse lung epithelial cell line. None of these particles induced cell death within 24 hr at doses between 0 and 200 microg/ml or during long-term subculture exposure (576 hr) at 100 microg/ml, as determined by two different assays. However, cell proliferation was slower with SWCNT exposure and a larger fraction of the cells were in the G1 phase. Exposure to carbon black resulted in the greatest reactive oxygen species generation followed by SWCNT and C(60) in both cellular and cell-free particle suspensions. C(60) and SWCNT did not increase the level of strand breaks, but significantly increased the level of FPG sensitive sites/oxidized purines (22 and 56%, respectively) determined by the comet assay. The mutant frequency in the cII gene was unaffected by 576 hr of exposure to either 100 microg/ml C(60) or SWCNT when compared with control incubations, whereas we have previously reported that carbon black and diesel exhaust particles induce mutations using an identical exposure scenario. These results indicate that SWCNT and C(60) are less genotoxic in vitro than carbon black and diesel exhaust particles.
U2 - 10.1002/em.20406
DO - 10.1002/em.20406
M3 - Journal article
C2 - 18618583
VL - 49
SP - 476
EP - 487
JO - Environmental and Molecular Mutagenesis
JF - Environmental and Molecular Mutagenesis
SN - 0893-6692
IS - 6
ER -