TY - JOUR
T1 - Good performance of an immunoassay based method for nevirapine measurements in human breast milk
AU - Salado-Rasmussen, Kirsten
AU - Persson Theilgaard, Zahra
AU - Chiduo, Mercy
AU - Pedersen, Court
AU - Gerstoft, Jan
AU - Katzenstein, Terese Lea
PY - 2011/7/1
Y1 - 2011/7/1
N2 - Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 µL) and no pre-treatment of the samples.
AB - Understanding the distribution of antiretro-virals in breastfeeding HIV-positive mothers is essential, both for prevention of mother-to-child HIV transmission and for research on the development of drug resistance. The ARK nevirapine (NVP)-test is an immunoassay method for nevirapine measurements, developed and validated for plasma use. In this study, the ARK NVP-test was evaluated for measurement of nevirapine concentrations in breast milk. High performance liquid chromatography (HPLC) is the method currently used to determine nevirapine in breast milk. This method, however, requires complicated extraction techniques. The ARK method employs an immunoassay technology and requires a small sample volume (40 µL) and no pre-treatment of the samples.
U2 - 10.1515/CCLM.2011.184
DO - 10.1515/CCLM.2011.184
M3 - Journal article
SN - 1434-6621
VL - 49
SP - 1171
EP - 1175
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
IS - 7
ER -