TY - JOUR
T1 - GPR183 is Dispensable for B1 cell Accumulation and Function, but Affects B2 cell Abundance, in the Omentum and Peritoneal Cavity
AU - Barington, Line
AU - Christensen, Liv von Voss
AU - Pedersen, Kristian Kåber
AU - Arfelt, Kristine Niss
AU - Roumain, Martin
AU - Jensen, Kristian Høj Reveles
AU - Kjær, Viktoria Madeline Skovgaard
AU - Daugvilaite, Viktorija
AU - Kearney, John F.
AU - Christensen, Jan Pravsgaard
AU - Hjortø, Gertrud Malene
AU - Muccioli, Giulio G.
AU - Holst, Peter Johannes
AU - Rosenkilde, Mette Marie
N1 - Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022
Y1 - 2022
N2 - B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abun-dant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a-or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.
AB - B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abun-dant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a-or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.
KW - 7TM receptor
KW - B1 cell, B-1 cell
KW - GPCR
KW - GPR183, EBI2
KW - Oxysterol
UR - http://www.scopus.com/inward/record.url?scp=85123573326&partnerID=8YFLogxK
U2 - 10.3390/cells11030494
DO - 10.3390/cells11030494
M3 - Journal article
C2 - 35159303
AN - SCOPUS:85123573326
VL - 11
JO - Cells
JF - Cells
SN - 2073-4409
IS - 3
M1 - 494
ER -