Greater molecular potential for glucose metabolism in adipose tissue and skeletal muscle of women compared with men

Trine S. Nicolaisen, Kim A. Sjøberg, Christian S. Carl, Erik A. Richter, Bente Kiens*, Andreas M. Fritzen, Anne Marie Lundsgaard

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

2 Downloads (Pure)

Abstract

Women typically have less muscle mass and more fat mass than men, while at the same time possessing similar or even greater whole-body insulin sensitivity. Our study aimed to investigate the molecular factors in primarily adipose tissue, but also in skeletal muscle, contributing to this sex difference. In healthy, moderately active premenopausal women and men with normal weight (28 ± 5 and 23 ± 3 years old; BMI 22.2 ± 1.9 and 23.7 ± 1.7) and in healthy, recreationally active women and men with overweight (32.2 ± 6 and 31.0 ± 5 years old; BMI 29.8 ± 4.3 & 30.9 ± 3.7) matched at age, BMI, and fitness level, we assessed insulin sensitivity and glucose tolerance with a hyperinsulinemic–euglycemic clamp or oral glucose tolerance test and studied subcutaneous adipose tissue and skeletal muscle samples with western blotting. Additionally, we traced glucose-stimulated glucose disposal in adipose tissues of female and male C57BL/6J littermate mice aged 16 weeks and measured glucose metabolic proteins. Our findings revealed greater protein expression related to glucose disposal in the subcutaneous adipose tissue (AKT2, insulin receptor, glucose transport 4) and skeletal muscle (hexokinase II, pyruvate dehydrogenase) in women compared to matched men with normal weight and with overweight. This increased protein capacity for glucose uptake extended to white adipose tissues of mice accompanied with ~2-fold greater glucose uptake compared to male mice. Furthermore, even in the obese state, women displayed better glucose tolerance than matched men, despite having 46% body fat and 20 kg less lean mass. In conclusion, our findings suggest that the superior potential for glucose disposal in female subcutaneous adipose tissue and skeletal muscle, driven by greater expression of various glucose metabolic proteins, compensates for their lower muscle mass. This likely explains women's superior glucose tolerance and tissue insulin sensitivity compared to men.

OriginalsprogEngelsk
Artikelnummere23845
TidsskriftFASEB Journal
Vol/bind38
Udgave nummer15
Antal sider14
ISSN0892-6638
DOI
StatusUdgivet - 2024

Bibliografisk note

Funding Information:
B.K. and E.A.R were funded by The University of Copenhagen Excellence Program for Interdisciplinary Research (2016): \u201CPhysical activity and Nutrition for Improvement of Health\u201D and the Independent Research Fund Denmark \u2013 Medical Sciences (grant: 4183\u201000249). A\u2010M.L. and A.M.F. were supported by a postdoctoral research grant from the Danish Diabetes Academy, funded by the Novo Nordisk Foundation, grant number NNF17SA0031406. Furthermore, A.M.F. was supported by the Novo Nordisk Foundation, grant number NNF22OC0074110. K.A.S was supported by a postdoctoral research grant from the Independent Research Fund Denmark \u2013 Medical Sciences, grant number 4092\u201000309.

Publisher Copyright:
© 2024 The Author(s). The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.

Citationsformater