TY - JOUR
T1 - Growth kinetics of four human breast carcinomas grown in nude mice
AU - Spang-Thomsen, M
AU - Rygaard, K
AU - Hansen, L
AU - Halvorsen, A C
AU - Vindeløv, L L
AU - Brünner, N
N1 - Keywords: Animals; Breast Neoplasms; Cell Cycle; DNA, Neoplasm; Flow Cytometry; Humans; Mice; Mice, Nude; Mitotic Index; Neoplasm Transplantation; Receptors, Estrogen; Receptors, Progesterone; Tumor Cells, Cultured
PY - 1989
Y1 - 1989
N2 - The immune-deficient nude mouse with human tumor xenografts is an appropriate model system for performing detailed growth kinetic examinations. In the present study one estrogen and progesterone receptor-negative (T60) and three receptor-positive (Br-10, MCF-7, T61) human breast cancer xenografts in nude mice were investigated. The proliferative tumor characteristics were examined by growth curves, thymidine labelling technique, and flow cytometric DNA analysis performed on fine-needle aspirations. The results showed that the tumors had growth kinetics comparable to other human tumor types with cell generation times of 42 to 60 hours. The three receptor-positive tumors had slower growth rate, larger tumor volume doubling time, and smaller growth fraction and labelling index than the receptor-negative tumor. However, no single proliferation parameter was sufficient to characterize the growth kinetics of individual tumors or to describe proliferative differences between the tumors.
AB - The immune-deficient nude mouse with human tumor xenografts is an appropriate model system for performing detailed growth kinetic examinations. In the present study one estrogen and progesterone receptor-negative (T60) and three receptor-positive (Br-10, MCF-7, T61) human breast cancer xenografts in nude mice were investigated. The proliferative tumor characteristics were examined by growth curves, thymidine labelling technique, and flow cytometric DNA analysis performed on fine-needle aspirations. The results showed that the tumors had growth kinetics comparable to other human tumor types with cell generation times of 42 to 60 hours. The three receptor-positive tumors had slower growth rate, larger tumor volume doubling time, and smaller growth fraction and labelling index than the receptor-negative tumor. However, no single proliferation parameter was sufficient to characterize the growth kinetics of individual tumors or to describe proliferative differences between the tumors.
M3 - Journal article
C2 - 2605350
SN - 0167-6806
VL - 14
SP - 235
EP - 243
JO - Breast Cancer Research and Treatment
JF - Breast Cancer Research and Treatment
IS - 2
ER -