Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | British Journal of Cancer |
| Vol/bind | 69 |
| Udgave nummer | 5 |
| Sider (fra-til) | 802-8 |
| Antal sider | 6 |
| ISSN | 0007-0920 |
| Status | Udgivet - 1994 |
Bibliografisk note
Keywords: Carcinoma, Small Cell; Cell Division; Cell Nucleus; Humans; Lung Neoplasms; Receptors, Transforming Growth Factor beta; Retinoblastoma Protein; Transforming Growth Factor beta; Tumor Cells, CulturedBiblioteksadgang?
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I: British Journal of Cancer, Bind 69, Nr. 5, 1994, s. 802-8.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
}
TY - JOUR
T1 - Growth suppression by transforming growth factor beta 1 of human small-cell lung cancer cell lines is associated with expression of the type II receptor
AU - Nørgaard, P
AU - Damstrup, L
AU - Rygaard, K
AU - Spang-Thomsen, M
AU - Skovgaard Poulsen, H
N1 - Keywords: Carcinoma, Small Cell; Cell Division; Cell Nucleus; Humans; Lung Neoplasms; Receptors, Transforming Growth Factor beta; Retinoblastoma Protein; Transforming Growth Factor beta; Tumor Cells, Cultured
PY - 1994
Y1 - 1994
N2 - Nine human small-cell lung cancer cell lines were treated with transforming growth factor beta 1 (TGF-beta 1). Seven of the cell lines expressed receptors for transforming growth factor beta (TGF-beta-r) in different combinations between the three human subtypes I, II and III, and two were receptor negative. Growth suppression was induced by TGF-beta 1 exclusively in the five cell lines expressing the type II receptor. For the first time growth suppression by TGF-beta 1 of a cell line expressing the type II receptor without coexpression of the type I receptor is reported. No effect on growth was observed in two cell lines expressing only type III receptor and in TGF-beta-r negative cell lines. In two cell lines expressing all three receptor types, growth suppression was accompanied by morphological changes. To evaluate the possible involvement of the retinoblastoma protein (pRb) in mediating the growth-suppressive effect of TGF-beta 1, the expression of functional pRb, as characterised by nuclear localisation, was examined by immunocytochemistry. Nuclear association of pRb was only seen in two of the five TGF-beta 1-responsive cell lines. These results indicate that in SCLC pRb is not required for mediation of TGF-beta 1-induced growth suppression.
AB - Nine human small-cell lung cancer cell lines were treated with transforming growth factor beta 1 (TGF-beta 1). Seven of the cell lines expressed receptors for transforming growth factor beta (TGF-beta-r) in different combinations between the three human subtypes I, II and III, and two were receptor negative. Growth suppression was induced by TGF-beta 1 exclusively in the five cell lines expressing the type II receptor. For the first time growth suppression by TGF-beta 1 of a cell line expressing the type II receptor without coexpression of the type I receptor is reported. No effect on growth was observed in two cell lines expressing only type III receptor and in TGF-beta-r negative cell lines. In two cell lines expressing all three receptor types, growth suppression was accompanied by morphological changes. To evaluate the possible involvement of the retinoblastoma protein (pRb) in mediating the growth-suppressive effect of TGF-beta 1, the expression of functional pRb, as characterised by nuclear localisation, was examined by immunocytochemistry. Nuclear association of pRb was only seen in two of the five TGF-beta 1-responsive cell lines. These results indicate that in SCLC pRb is not required for mediation of TGF-beta 1-induced growth suppression.
M3 - Journal article
C2 - 8180008
SN - 0007-0920
VL - 69
SP - 802
EP - 808
JO - British Journal of Cancer
JF - British Journal of Cancer
IS - 5
ER -