Abstract
Roux-en-Y gastric bypass surgery (RYGB) leads to improved glycemic control
in individuals with severe obesity beyond the effects of weight loss alone. Here, We
addressed the potential contribution of gut microbiota in mediating this favourable surgical
outcome by using an established preclinical model of RYGB. 16S rRNA sequencing
revealed that RYGB-treated Zucker fatty rats had altered fecal composition of various
bacteria at the phylum and species levels, including lower fecal abundance of an unidentified
Erysipelotrichaceae species, compared with both sham-operated (Sham) and
body weight-matched to RYGB-treated (BWM) rats. Correlation analysis further revealed
that fecal abundance of this unidentified Erysipelotrichaceae species linked with multiple
indices of glycemic control uniquely in RYGB-treated rats. Sequence alignment of this
Erysipelotrichaceae species identified Longibaculum muris to be the most closely related
species, and its fecal abundance positively correlated with oral glucose intolerance in
RYGB-treated rats. In fecal microbiota transplant experiments, the improved oral glucose
tolerance of RYGB-treated compared with BWM rats could partially be transferred to recipient
germfree mice, independently of body weight. Unexpectedly, providing L. muris
as a supplement to RYGB recipient mice further improved oral glucose tolerance, while
administering L. muris alone to chow-fed or Western style diet-challenged conventionally
raised mice had minimal metabolic impact. Taken together, our findings provide evidence
that the gut microbiota contributes to weight loss-independent improvements in
glycemic control after RYGB and demonstrate how correlation of a specific gut microbiota
species with a host metabolic trait does not imply causation.
in individuals with severe obesity beyond the effects of weight loss alone. Here, We
addressed the potential contribution of gut microbiota in mediating this favourable surgical
outcome by using an established preclinical model of RYGB. 16S rRNA sequencing
revealed that RYGB-treated Zucker fatty rats had altered fecal composition of various
bacteria at the phylum and species levels, including lower fecal abundance of an unidentified
Erysipelotrichaceae species, compared with both sham-operated (Sham) and
body weight-matched to RYGB-treated (BWM) rats. Correlation analysis further revealed
that fecal abundance of this unidentified Erysipelotrichaceae species linked with multiple
indices of glycemic control uniquely in RYGB-treated rats. Sequence alignment of this
Erysipelotrichaceae species identified Longibaculum muris to be the most closely related
species, and its fecal abundance positively correlated with oral glucose intolerance in
RYGB-treated rats. In fecal microbiota transplant experiments, the improved oral glucose
tolerance of RYGB-treated compared with BWM rats could partially be transferred to recipient
germfree mice, independently of body weight. Unexpectedly, providing L. muris
as a supplement to RYGB recipient mice further improved oral glucose tolerance, while
administering L. muris alone to chow-fed or Western style diet-challenged conventionally
raised mice had minimal metabolic impact. Taken together, our findings provide evidence
that the gut microbiota contributes to weight loss-independent improvements in
glycemic control after RYGB and demonstrate how correlation of a specific gut microbiota
species with a host metabolic trait does not imply causation.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Microbiology Spectrum |
| Vol/bind | 11 |
| Udgave nummer | 3 |
| Antal sider | 9 |
| ISSN | 2165-0497 |
| DOI | |
| Status | Udgivet - 2023 |
Bibliografisk note
Funding Information:This research was funded by the Interdisciplinary Center for Clinical Research of Wuerzburg (IZKF) (grant number Z-3/44 for F.S.) and the Novo Nordisk Foundation (NNF15OC0016798 for T.A.).
Publisher Copyright:
Copyright © 2023 Hankir et al.