Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization.

Jesper Nylandsted; Mads Gyrd-Hansen; Agnieszka Danielewicz; Nicole Fehrenbacher; Ulrik Lademann; Maria Høyer-Hansen; Ekkehard Weber; Gabriele Multhoff; Mikkel Rohde; Marja Jäättelä

doi:10.1084/jem.20040531

Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization.

Jesper Nylandsted, Mads Gyrd-Hansen, Agnieszka Danielewicz, Nicole Fehrenbacher, Ulrik Lademann, Maria Høyer-Hansen, Ekkehard Weber, Gabriele Multhoff, Mikkel Rohde, Marja Jäättelä

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

480 Citationer (Scopus)

Abstract

Udgivelsesdato: 2004-Aug-16

Originalsprog	Engelsk
Tidsskrift	Journal of Experimental Medicine
Vol/bind	200
Udgave nummer	4
Sider (fra-til)	425-435
Antal sider	11
ISSN	0022-1007
DOI	https://doi.org/10.1084/jem.20040531
Status	Udgivet - 2004
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Animals; Apoptosis; Caspases; Cathepsins; Cell Membrane Permeability; Cell Survival; HSP70 Heat-Shock Proteins; Hela Cells; Humans; Immunoblotting; Lysosomes; Mice; Microscopy, Immunoelectron; Tumor Cells, Cultured

Adgang til dokumentet

10.1084/jem.20040531

Citationsformater

Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization. / Nylandsted, Jesper; Gyrd-Hansen, Mads; Danielewicz, Agnieszka; Fehrenbacher, Nicole; Lademann, Ulrik; Høyer-Hansen, Maria; Weber, Ekkehard; Multhoff, Gabriele; Rohde, Mikkel; Jäättelä, Marja.

I: Journal of Experimental Medicine, Bind 200, Nr. 4, 2004, s. 425-435.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Nylandsted, Jesper ; Gyrd-Hansen, Mads ; Danielewicz, Agnieszka ; Fehrenbacher, Nicole ; Lademann, Ulrik ; Høyer-Hansen, Maria ; Weber, Ekkehard ; Multhoff, Gabriele ; Rohde, Mikkel ; Jäättelä, Marja. / Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization. I: Journal of Experimental Medicine. 2004 ; Bind 200, Nr. 4. s. 425-435.

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title = "Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization.",

abstract = "Heat shock protein 70 (Hsp70) is a potent survival protein whose depletion triggers massive caspase-independent tumor cell death. Here, we show that Hsp70 exerts its prosurvival function by inhibiting lysosomal membrane permeabilization. The cell death induced by Hsp70 depletion was preceded by the release of lysosomal enzymes into the cytosol and inhibited by pharmacological inhibitors of lysosomal cysteine proteases. Accordingly, the Hsp70-mediated protection against various death stimuli in Hsp70-expressing human tumor cells as well as in immortalized Hsp70 transgenic murine fibroblasts occurred at the level of the lysosomal permeabilization. On the contrary, Hsp70 failed to inhibit the cytochrome c-induced, apoptosome-dependent caspase activation in vitro and Fas ligand-induced, caspase-dependent apoptosis in immortalized fibroblasts. Immunoelectron microscopy revealed that endosomal and lysosomal membranes of tumor cells contained Hsp70. Permeabilization of purified endo/lysosomes by digitonin failed to release Hsp70, suggesting that it is physically associated with the membranes. Finally, Hsp70 positive lysosomes displayed increased size and resistance against chemical and physical membrane destabilization. These data identify Hsp70 as the first survival protein that functions by inhibiting the death-associated permeabilization of lysosomes.",

author = "Jesper Nylandsted and Mads Gyrd-Hansen and Agnieszka Danielewicz and Nicole Fehrenbacher and Ulrik Lademann and Maria H{\o}yer-Hansen and Ekkehard Weber and Gabriele Multhoff and Mikkel Rohde and Marja J{\"a}{\"a}ttel{\"a}",

note = "Keywords: Animals; Apoptosis; Caspases; Cathepsins; Cell Membrane Permeability; Cell Survival; HSP70 Heat-Shock Proteins; Hela Cells; Humans; Immunoblotting; Lysosomes; Mice; Microscopy, Immunoelectron; Tumor Cells, Cultured",

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T1 - Heat shock protein 70 promotes cell survival by inhibiting lysosomal membrane permeabilization.

AU - Nylandsted, Jesper

AU - Gyrd-Hansen, Mads

AU - Danielewicz, Agnieszka

AU - Fehrenbacher, Nicole

AU - Lademann, Ulrik

AU - Høyer-Hansen, Maria

AU - Weber, Ekkehard

AU - Multhoff, Gabriele

AU - Rohde, Mikkel

AU - Jäättelä, Marja

N1 - Keywords: Animals; Apoptosis; Caspases; Cathepsins; Cell Membrane Permeability; Cell Survival; HSP70 Heat-Shock Proteins; Hela Cells; Humans; Immunoblotting; Lysosomes; Mice; Microscopy, Immunoelectron; Tumor Cells, Cultured

PY - 2004

Y1 - 2004

N2 - Heat shock protein 70 (Hsp70) is a potent survival protein whose depletion triggers massive caspase-independent tumor cell death. Here, we show that Hsp70 exerts its prosurvival function by inhibiting lysosomal membrane permeabilization. The cell death induced by Hsp70 depletion was preceded by the release of lysosomal enzymes into the cytosol and inhibited by pharmacological inhibitors of lysosomal cysteine proteases. Accordingly, the Hsp70-mediated protection against various death stimuli in Hsp70-expressing human tumor cells as well as in immortalized Hsp70 transgenic murine fibroblasts occurred at the level of the lysosomal permeabilization. On the contrary, Hsp70 failed to inhibit the cytochrome c-induced, apoptosome-dependent caspase activation in vitro and Fas ligand-induced, caspase-dependent apoptosis in immortalized fibroblasts. Immunoelectron microscopy revealed that endosomal and lysosomal membranes of tumor cells contained Hsp70. Permeabilization of purified endo/lysosomes by digitonin failed to release Hsp70, suggesting that it is physically associated with the membranes. Finally, Hsp70 positive lysosomes displayed increased size and resistance against chemical and physical membrane destabilization. These data identify Hsp70 as the first survival protein that functions by inhibiting the death-associated permeabilization of lysosomes.

AB - Heat shock protein 70 (Hsp70) is a potent survival protein whose depletion triggers massive caspase-independent tumor cell death. Here, we show that Hsp70 exerts its prosurvival function by inhibiting lysosomal membrane permeabilization. The cell death induced by Hsp70 depletion was preceded by the release of lysosomal enzymes into the cytosol and inhibited by pharmacological inhibitors of lysosomal cysteine proteases. Accordingly, the Hsp70-mediated protection against various death stimuli in Hsp70-expressing human tumor cells as well as in immortalized Hsp70 transgenic murine fibroblasts occurred at the level of the lysosomal permeabilization. On the contrary, Hsp70 failed to inhibit the cytochrome c-induced, apoptosome-dependent caspase activation in vitro and Fas ligand-induced, caspase-dependent apoptosis in immortalized fibroblasts. Immunoelectron microscopy revealed that endosomal and lysosomal membranes of tumor cells contained Hsp70. Permeabilization of purified endo/lysosomes by digitonin failed to release Hsp70, suggesting that it is physically associated with the membranes. Finally, Hsp70 positive lysosomes displayed increased size and resistance against chemical and physical membrane destabilization. These data identify Hsp70 as the first survival protein that functions by inhibiting the death-associated permeabilization of lysosomes.

U2 - 10.1084/jem.20040531

DO - 10.1084/jem.20040531

M3 - Journal article

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VL - 200

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JO - The Journal of Experimental Medicine

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