Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Clinical Cancer Research |
| Vol/bind | 15 |
| Udgave nummer | 9 |
| Sider (fra-til) | 2995-3002 |
| Antal sider | 7 |
| ISSN | 1078-0432 |
| DOI | |
| Status | Udgivet - 2009 |
Bibliografisk note
Keywords: Adenocarcinoma; Adult; Animals; Apoptosis; Blotting, Western; Cell Nucleus; Cell Proliferation; Cells, Cultured; DNA Repair; DNA, Mitochondrial; Dinucleotide Repeats; Female; Genomic Instability; Helicobacter Infections; Helicobacter pylori; Humans; Male; Mice; Mice, Inbred C57BL; Middle Aged; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Stomach NeoplasmsAdgang til dokumentet
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I: Clinical Cancer Research, Bind 15, Nr. 9, 2009, s. 2995-3002.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Helicobacter pylori infection induces genetic instability of nuclear and mitochondrial DNA in gastric cells
AU - Machado, Ana Manuel Dantas
AU - Figueiredo, Ceu
AU - Touati, Eliette
AU - Máximo, Valdemar
AU - Sousa, Sonia
AU - Michel, Valérie
AU - Carneiro, Fátima
AU - Nielsen, Finn Cilius
AU - Seruca, Raquel
AU - Rasmussen, Lene Juel
N1 - Keywords: Adenocarcinoma; Adult; Animals; Apoptosis; Blotting, Western; Cell Nucleus; Cell Proliferation; Cells, Cultured; DNA Repair; DNA, Mitochondrial; Dinucleotide Repeats; Female; Genomic Instability; Helicobacter Infections; Helicobacter pylori; Humans; Male; Mice; Mice, Inbred C57BL; Middle Aged; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms
PY - 2009
Y1 - 2009
N2 - PURPOSE: Helicobacter pylori is a major cause of gastric carcinoma. To investigate a possible link between bacterial infection and genetic instability of the host genome, we examined the effect of H. pylori infection on known cellular repair pathways in vitro and in vivo. Moreover, various types of genetic instabilities in the nuclear and mitochondrial DNA (mtDNA) were examined. EXPERIMENTAL DESIGN: We observed the effects of H. pylori infection on a gastric cell line (AGS), on C57BL/6 mice, and on individuals with chronic gastritis. In AGS cells, the effect of H. pylori infection on base excision repair and mismatch repair (MMR) was analyzed by reverse transcription-PCR, Western blot, and activity assays. In mice, MMR expression was analyzed by reverse transcription-PCR and the CA repeat instabilities were examined by Mutation Detection Enhancement gel electrophoresis. Mutation spectra in AGS cells and chronic gastritis tissue were determined by PCR, single-stranded conformation polymorphism, and sequencing. H. pylori vacA and cagA genotyping was determined by multiplex PCR and reverse hybridization. RESULTS: Following H. pylori infection, the activity and expression of base excision repair and MMR are down-regulated both in vitro and in vivo. Moreover, H. pylori induces genomic instability in nuclear CA repeats in mice and in mtDNA of AGS cells and chronic gastritis tissue, and this effect in mtDNA is associated with bacterial virulence. CONCLUSIONS: Our results suggest that H. pylori impairs central DNA repair mechanisms, inducing a transient mutator phenotype, rendering gastric epithelial cells vulnerable to the accumulation of genetic instability and thus contributing to gastric carcinogenesis in infected individuals.
AB - PURPOSE: Helicobacter pylori is a major cause of gastric carcinoma. To investigate a possible link between bacterial infection and genetic instability of the host genome, we examined the effect of H. pylori infection on known cellular repair pathways in vitro and in vivo. Moreover, various types of genetic instabilities in the nuclear and mitochondrial DNA (mtDNA) were examined. EXPERIMENTAL DESIGN: We observed the effects of H. pylori infection on a gastric cell line (AGS), on C57BL/6 mice, and on individuals with chronic gastritis. In AGS cells, the effect of H. pylori infection on base excision repair and mismatch repair (MMR) was analyzed by reverse transcription-PCR, Western blot, and activity assays. In mice, MMR expression was analyzed by reverse transcription-PCR and the CA repeat instabilities were examined by Mutation Detection Enhancement gel electrophoresis. Mutation spectra in AGS cells and chronic gastritis tissue were determined by PCR, single-stranded conformation polymorphism, and sequencing. H. pylori vacA and cagA genotyping was determined by multiplex PCR and reverse hybridization. RESULTS: Following H. pylori infection, the activity and expression of base excision repair and MMR are down-regulated both in vitro and in vivo. Moreover, H. pylori induces genomic instability in nuclear CA repeats in mice and in mtDNA of AGS cells and chronic gastritis tissue, and this effect in mtDNA is associated with bacterial virulence. CONCLUSIONS: Our results suggest that H. pylori impairs central DNA repair mechanisms, inducing a transient mutator phenotype, rendering gastric epithelial cells vulnerable to the accumulation of genetic instability and thus contributing to gastric carcinogenesis in infected individuals.
U2 - 10.1158/1078-0432.CCR-08-2686
DO - 10.1158/1078-0432.CCR-08-2686
M3 - Journal article
C2 - 19383819
SN - 1078-0432
VL - 15
SP - 2995
EP - 3002
JO - Clinical Cancer Research
JF - Clinical Cancer Research
IS - 9
ER -