Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

Tine Rosenberg; Charlotte Aaberg-Jessen; Stine Asferg Petterson; Bjarne Winther Kristensen

doi:10.2217/cns-2017-0034

Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

Tine Rosenberg, Charlotte Aaberg-Jessen, Stine Asferg Petterson, Bjarne Winther Kristensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

6 Citationer (Scopus)

Abstract

AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.

MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.

RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.

CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

Originalsprog	Engelsk
Tidsskrift	CNS oncology
Vol/bind	7
Udgave nummer	2
Sider (fra-til)	CNS15
ISSN	2045-0907
DOI	https://doi.org/10.2217/cns-2017-0034
Status	Udgivet - apr. 2018
Udgivet eksternt	Ja

Adgang til dokumentet

10.2217/cns-2017-0034

Citationsformater

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title = "Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia",

abstract = "AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.",

author = "Tine Rosenberg and Charlotte Aaberg-Jessen and Petterson, {Stine Asferg} and Kristensen, {Bjarne Winther}",

year = "2018",

month = apr,

doi = "10.2217/cns-2017-0034",

language = "English",

volume = "7",

pages = "CNS15",

journal = "CNS oncology",

issn = "2045-0907",

publisher = "Future Medicine Ltd.",

number = "2",

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TY - JOUR

T1 - Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

AU - Rosenberg, Tine

AU - Aaberg-Jessen, Charlotte

AU - Petterson, Stine Asferg

AU - Kristensen, Bjarne Winther

PY - 2018/4

Y1 - 2018/4

N2 - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

AB - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

U2 - 10.2217/cns-2017-0034

DO - 10.2217/cns-2017-0034

M3 - Journal article

C2 - 29708435

SN - 2045-0907

VL - 7

SP - CNS15

JO - CNS oncology

JF - CNS oncology

IS - 2

ER -