High circulating levels of tumor necrosis factor-alpha in centenarians are not associated with increased production in T lymphocytes

Marie Sandmand; Helle Bruunsgaard; Kåre Kemp; Karen Andersen-Ranberg; Marianne Schroll; Bernard Jeune

doi:10.1159/000069174

High circulating levels of tumor necrosis factor-alpha in centenarians are not associated with increased production in T lymphocytes

Marie Sandmand, Helle Bruunsgaard, Kåre Kemp, Karen Andersen-Ranberg, Marianne Schroll, Bernard Jeune

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

32 Citationer (Scopus)

Abstract

Udgivelsesdato: null-null

Originalsprog	Engelsk
Tidsskrift	Gerontology
Vol/bind	49
Udgave nummer	3
Sider (fra-til)	155-60
Antal sider	5
ISSN	0304-324X
DOI	https://doi.org/10.1159/000069174
Status	Udgivet - 2003

Bibliografisk note

Adgang til dokumentet

10.1159/000069174

Citationsformater

High circulating levels of tumor necrosis factor-alpha in centenarians are not associated with increased production in T lymphocytes. / Sandmand, Marie; Bruunsgaard, Helle; Kemp, Kåre; Andersen-Ranberg, Karen; Schroll, Marianne; Jeune, Bernard.

I: Gerontology, Bind 49, Nr. 3, 2003, s. 155-60.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{084b31701a4711df8ed1000ea68e967b,

title = "High circulating levels of tumor necrosis factor-alpha in centenarians are not associated with increased production in T lymphocytes",

abstract = "BACKGROUND: Aging is characterized by increased inflammatory activity reflected by increased plasma levels of proinflammatory cytokines, concomitant with an altered cytokine profile of T lymphocytes. High plasma levels of tumor necrosis factor (TNF)-alpha are strongly associated with morbidity and mortality in elderly humans. However, the cellular source and mechanisms for the increased circulating TNF-alpha levels are unknown. OBJECTIVE: The aim of the present study was to investigate if high plasma levels of TNF-alpha are associated with increased production of TNF-alpha by T lymphocytes in elderly humans. METHODS: TNF-alpha production by CD4+ and CD8+ T lymphocytes was measured by flow cytometry following stimulation with phorbol 12-myristate 13-acetate and ionomycin in 28 young controls, 14, 81-year-olds and 25 centenarians. RESULTS: Plasma levels of TNF-alpha increased with increasing age. An increased percentage and number of T lymphocytes from the 81 year olds expressed TNF-alpha, whereas centenarians did not show this altered TNF-alpha secretion profile. CONCLUSION: T cells may contribute to the elevated levels of plasma TNF-alpha in healthy elderly subjects, whereas other mechanisms are responsible in very old individuals.",

author = "Marie Sandmand and Helle Bruunsgaard and K{\aa}re Kemp and Karen Andersen-Ranberg and Marianne Schroll and Bernard Jeune",

note = "Keywords: Adult; Aged; Aged, 80 and over; Female; Humans; Lymphocyte Activation; Male; T-Lymphocytes; Tumor Necrosis Factor-alpha",

year = "2003",

doi = "10.1159/000069174",

language = "English",

volume = "49",

pages = "155--60",

journal = "Gerontology",

issn = "0304-324X",

publisher = "S Karger AG",

number = "3",

}

TY - JOUR

T1 - High circulating levels of tumor necrosis factor-alpha in centenarians are not associated with increased production in T lymphocytes

AU - Sandmand, Marie

AU - Bruunsgaard, Helle

AU - Kemp, Kåre

AU - Andersen-Ranberg, Karen

AU - Schroll, Marianne

AU - Jeune, Bernard

N1 - Keywords: Adult; Aged; Aged, 80 and over; Female; Humans; Lymphocyte Activation; Male; T-Lymphocytes; Tumor Necrosis Factor-alpha

PY - 2003

Y1 - 2003

N2 - BACKGROUND: Aging is characterized by increased inflammatory activity reflected by increased plasma levels of proinflammatory cytokines, concomitant with an altered cytokine profile of T lymphocytes. High plasma levels of tumor necrosis factor (TNF)-alpha are strongly associated with morbidity and mortality in elderly humans. However, the cellular source and mechanisms for the increased circulating TNF-alpha levels are unknown. OBJECTIVE: The aim of the present study was to investigate if high plasma levels of TNF-alpha are associated with increased production of TNF-alpha by T lymphocytes in elderly humans. METHODS: TNF-alpha production by CD4+ and CD8+ T lymphocytes was measured by flow cytometry following stimulation with phorbol 12-myristate 13-acetate and ionomycin in 28 young controls, 14, 81-year-olds and 25 centenarians. RESULTS: Plasma levels of TNF-alpha increased with increasing age. An increased percentage and number of T lymphocytes from the 81 year olds expressed TNF-alpha, whereas centenarians did not show this altered TNF-alpha secretion profile. CONCLUSION: T cells may contribute to the elevated levels of plasma TNF-alpha in healthy elderly subjects, whereas other mechanisms are responsible in very old individuals.

AB - BACKGROUND: Aging is characterized by increased inflammatory activity reflected by increased plasma levels of proinflammatory cytokines, concomitant with an altered cytokine profile of T lymphocytes. High plasma levels of tumor necrosis factor (TNF)-alpha are strongly associated with morbidity and mortality in elderly humans. However, the cellular source and mechanisms for the increased circulating TNF-alpha levels are unknown. OBJECTIVE: The aim of the present study was to investigate if high plasma levels of TNF-alpha are associated with increased production of TNF-alpha by T lymphocytes in elderly humans. METHODS: TNF-alpha production by CD4+ and CD8+ T lymphocytes was measured by flow cytometry following stimulation with phorbol 12-myristate 13-acetate and ionomycin in 28 young controls, 14, 81-year-olds and 25 centenarians. RESULTS: Plasma levels of TNF-alpha increased with increasing age. An increased percentage and number of T lymphocytes from the 81 year olds expressed TNF-alpha, whereas centenarians did not show this altered TNF-alpha secretion profile. CONCLUSION: T cells may contribute to the elevated levels of plasma TNF-alpha in healthy elderly subjects, whereas other mechanisms are responsible in very old individuals.

U2 - 10.1159/000069174

DO - 10.1159/000069174

M3 - Journal article

C2 - 12679605

VL - 49

SP - 155

EP - 160

JO - Gerontology

JF - Gerontology

SN - 0304-324X

IS - 3

ER -