HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.

Jørgen Olsen; Lotte Laustsen; J Troelsen

HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.

Jørgen Olsen, Lotte Laustsen, J Troelsen

Institut for Cellulær og Molekylær Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

19 Citationer (Scopus)

Abstract

Udgivelsesdato: 1994-Apr-11

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	342
Udgave nummer	3
Sider (fra-til)	325-8
Antal sider	3
ISSN	0014-5793
Status	Udgivet - 1994

Bibliografisk note

Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Nucleus; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Hela Cells; Hepatocyte Nuclear Factor 1; Hepatocyte Nuclear Factor 1-alpha; Hepatocyte Nuclear Factor 1-beta; Humans; Intestinal Mucosa; Nuclear Proteins; Promoter Regions (Genetics); Protein Binding; Swine; Transcription Factors; Tumor Cells, Cultured

Citationsformater

@article{3e63ecc099fb11dd86a6000ea68e967b,

title = "HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.",

abstract = "The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.",

author = "J{\o}rgen Olsen and Lotte Laustsen and J Troelsen",

note = "Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Nucleus; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Hela Cells; Hepatocyte Nuclear Factor 1; Hepatocyte Nuclear Factor 1-alpha; Hepatocyte Nuclear Factor 1-beta; Humans; Intestinal Mucosa; Nuclear Proteins; Promoter Regions (Genetics); Protein Binding; Swine; Transcription Factors; Tumor Cells, Cultured",

year = "1994",

language = "English",

volume = "342",

pages = "325--8",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "JohnWiley & Sons Ltd",

number = "3",

}

TY - JOUR

T1 - HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.

AU - Olsen, Jørgen

AU - Laustsen, Lotte

AU - Troelsen, J

N1 - Keywords: Aminopeptidases; Animals; Antigens, CD13; Cell Nucleus; DNA-Binding Proteins; Gene Expression Regulation, Neoplastic; Hela Cells; Hepatocyte Nuclear Factor 1; Hepatocyte Nuclear Factor 1-alpha; Hepatocyte Nuclear Factor 1-beta; Humans; Intestinal Mucosa; Nuclear Proteins; Promoter Regions (Genetics); Protein Binding; Swine; Transcription Factors; Tumor Cells, Cultured

PY - 1994

Y1 - 1994

N2 - The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.

AB - The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation of the promoter by HNF1 alpha through this element. Electrophoretic mobility shift assays using nuclear extracts from Caco-2 cells show the presence of high amounts of HNF1 binding proteins irrespective of their state of differentiation.

M3 - Journal article

C2 - 7908650

SN - 0014-5793

VL - 342

SP - 325

EP - 328

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -