Human interferon-gamma quantified via a sensitive one-site monoclonal antibody in a sandwich-ELISA. A PEG-modification for measurements in serum

Kurt Berg

doi:10.1111/j.1699-0463.1994.tb04840.x

Human interferon-gamma quantified via a sensitive one-site monoclonal antibody in a sandwich-ELISA. A PEG-modification for measurements in serum

Kurt Berg

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning

6 Citationer (Scopus)

Abstract

Immune interferon, ELISA, *Gg-IFN

Originalsprog	Engelsk
Tidsskrift	A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
Vol/bind	102
Udgave nummer	1
Sider (fra-til)	13-22
ISSN	0903-4641
DOI	https://doi.org/10.1111/j.1699-0463.1994.tb04840.x
Status	Udgivet - 1994

Adgang til dokumentet

10.1111/j.1699-0463.1994.tb04840.x

Citationsformater

@article{8dc710c074ce11dbbee902004c4f4f50,

title = "Human interferon-gamma quantified via a sensitive one-site monoclonal antibody in a sandwich-ELISA. A PEG-modification for measurements in serum",

abstract = "A new, specific and sensitive one-site ELISA for precise quantification of human interferon-gamma (HuIFN-gamma) at low levels in 50% human serum samples has been developed. The assay is based on the assumption that biologically active HuIFN-gamma is present exclusively as a dimer. Thus, in contrast to previous reports, the ELISA is based on a single monoclonal antibody (MAb) which is used in two ways: as {"}catching{"} antibody and as HRPO-labelled conjugate. The sensitivity could be improved five-fold by addition of (NH4)2SO4 to the conjugate solution; the lowest detectable amount of HuIFN-gamma is < 0.5 mu/ml. Non-specific interactions were not seen in interferon samples taken from cultures, or samples diluted in ordinary media or 1% BSA. However, > 30% of the (serum) samples gave non-specific false-positive results when the method was applied to samples containing 50-100% human serum from different donors. The false signals were related to the donors but could-at the expense of the sensitivity which was reduced to 1 mu/ml-be abolished by PEG treatment of the (donor) serum samples.",

author = "Kurt Berg",

year = "1994",

doi = "10.1111/j.1699-0463.1994.tb04840.x",

language = "English",

volume = "102",

pages = "13--22",

journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",

issn = "0903-4641",

publisher = "Wiley Online",

number = "1",

}

TY - JOUR

T1 - Human interferon-gamma quantified via a sensitive one-site monoclonal antibody in a sandwich-ELISA. A PEG-modification for measurements in serum

AU - Berg, Kurt

PY - 1994

Y1 - 1994

N2 - A new, specific and sensitive one-site ELISA for precise quantification of human interferon-gamma (HuIFN-gamma) at low levels in 50% human serum samples has been developed. The assay is based on the assumption that biologically active HuIFN-gamma is present exclusively as a dimer. Thus, in contrast to previous reports, the ELISA is based on a single monoclonal antibody (MAb) which is used in two ways: as "catching" antibody and as HRPO-labelled conjugate. The sensitivity could be improved five-fold by addition of (NH4)2SO4 to the conjugate solution; the lowest detectable amount of HuIFN-gamma is < 0.5 mu/ml. Non-specific interactions were not seen in interferon samples taken from cultures, or samples diluted in ordinary media or 1% BSA. However, > 30% of the (serum) samples gave non-specific false-positive results when the method was applied to samples containing 50-100% human serum from different donors. The false signals were related to the donors but could-at the expense of the sensitivity which was reduced to 1 mu/ml-be abolished by PEG treatment of the (donor) serum samples.

AB - A new, specific and sensitive one-site ELISA for precise quantification of human interferon-gamma (HuIFN-gamma) at low levels in 50% human serum samples has been developed. The assay is based on the assumption that biologically active HuIFN-gamma is present exclusively as a dimer. Thus, in contrast to previous reports, the ELISA is based on a single monoclonal antibody (MAb) which is used in two ways: as "catching" antibody and as HRPO-labelled conjugate. The sensitivity could be improved five-fold by addition of (NH4)2SO4 to the conjugate solution; the lowest detectable amount of HuIFN-gamma is < 0.5 mu/ml. Non-specific interactions were not seen in interferon samples taken from cultures, or samples diluted in ordinary media or 1% BSA. However, > 30% of the (serum) samples gave non-specific false-positive results when the method was applied to samples containing 50-100% human serum from different donors. The false signals were related to the donors but could-at the expense of the sensitivity which was reduced to 1 mu/ml-be abolished by PEG treatment of the (donor) serum samples.

U2 - 10.1111/j.1699-0463.1994.tb04840.x

DO - 10.1111/j.1699-0463.1994.tb04840.x

M3 - Journal article

VL - 102

SP - 13

EP - 22

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 1

ER -