@article{e0e43940ba3111ddae57000ea68e967b,
title = "Human p53(264-272) HLA-A2 binding peptide is an immunodominant epitope in DNA-immunized HLA-A2 transgenic mice.",
abstract = "C57BL/10 mice transgenic for HLA-A2 were immunized with either a full-length DNA-construct of the tumor suppressor p53 or with a minigene encoding the p53-derived immunodominant peptide p53(264)LLGRNSFEV272 (L9V). Vaccination with the full-length p53 construct induced potent cytotoxic activity of splenocytes against L9V-pulsed target cells after in vivo re-stimulation. Vaccination with the L9V-encoding minigene likewise induced specific anti-L9V cytotoxicity in vitro. Subsequent experiments revealed that peptide-pulsed dendritic cells were the most efficient cell types for in vitro re-stimulation. In concordance with this, immunization with L9V-pulsed dendritic cells also induced a potent and specific anti-L9V cytotoxic response in vitro. These data show that HLA-A2/peptide-specific cytotoxic immunity can be generated in vivo against the same immunodominant epitope by immunizing either with full-length DNA or with a DNA minigene encoding the immunodominant peptide epitope.",
author = "Petersen, {T R} and S Bregenholta and Pedersen, {L O} and Nissen, {Mogens Holst} and Claesson, {M H}",
note = "Keywords: Animals; COS Cells; Cytotoxicity Tests, Immunologic; Dendritic Cells; Gene Expression; HLA-A2 Antigen; Humans; Immunity, Cellular; Immunodominant Epitopes; Mice; Mice, Inbred C57BL; Mice, Transgenic; Peptide Fragments; T-Lymphocytes, Cytotoxic; Transfection; Tumor Suppressor Protein p53; Vaccines, DNA",
year = "1999",
language = "English",
volume = "137",
pages = "183--91",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2",
}