Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.

Raino Kristian Hansen; Claus Christensen; Irina Korshunova; Martin Kriebel; Nadine Burkarth; Vladislav V Kiselyov; Marianne Olsen; Søren Ostergaard; Arne Holm; Hansjürgen Volkmer; Peter S Walmod; Vladimir Berezin; Elisabeth Bock

doi:10.1111/j.1471-4159.2007.04894.x

Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.

Raino Kristian Hansen, Claus Christensen, Irina Korshunova, Martin Kriebel, Nadine Burkarth, Vladislav V Kiselyov, Marianne Olsen, Søren Ostergaard, Arne Holm, Hansjürgen Volkmer, Peter S Walmod, Vladimir Berezin, Elisabeth Bock

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

21 Citationer (Scopus)

Abstract

Udgivelsesdato: 2007-Nov

Originalsprog	Engelsk
Tidsskrift	Journal of Neurochemistry
Vol/bind	103
Udgave nummer	4
Sider (fra-til)	1396-407
Antal sider	11
ISSN	0022-3042
DOI	https://doi.org/10.1111/j.1471-4159.2007.04894.x
Status	Udgivet - 2007

Bibliografisk note

Keywords: Animals; Cell Proliferation; Cells, Cultured; Cerebellum; Humans; Mice; Neural Cell Adhesion Molecules; Neurites; PC12 Cells; Peptides; Protein Binding; Rats; Rats, Wistar; Receptors, G-Protein-Coupled; Signal Transduction; Toxoids

Adgang til dokumentet

10.1111/j.1471-4159.2007.04894.x

Citationsformater

Hansen, R. K., Christensen, C., Korshunova, I., Kriebel, M., Burkarth, N., Kiselyov, V. V., Olsen, M., Ostergaard, S., Holm, A., Volkmer, H., Walmod, P. S., Berezin, V., & Bock, E. (2007). Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. Journal of Neurochemistry, 103(4), 1396-407. https://doi.org/10.1111/j.1471-4159.2007.04894.x

Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. / Hansen, Raino Kristian; Christensen, Claus; Korshunova, Irina; Kriebel, Martin; Burkarth, Nadine; Kiselyov, Vladislav V; Olsen, Marianne; Ostergaard, Søren; Holm, Arne; Volkmer, Hansjürgen; Walmod, Peter S; Berezin, Vladimir; Bock, Elisabeth.

I: Journal of Neurochemistry, Bind 103, Nr. 4, 2007, s. 1396-407.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Hansen, RK, Christensen, C, Korshunova, I, Kriebel, M, Burkarth, N, Kiselyov, VV, Olsen, M, Ostergaard, S, Holm, A, Volkmer, H, Walmod, PS, Berezin, V & Bock, E 2007, 'Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.', Journal of Neurochemistry, bind 103, nr. 4, s. 1396-407. https://doi.org/10.1111/j.1471-4159.2007.04894.x

Hansen, Raino Kristian ; Christensen, Claus ; Korshunova, Irina ; Kriebel, Martin ; Burkarth, Nadine ; Kiselyov, Vladislav V ; Olsen, Marianne ; Ostergaard, Søren ; Holm, Arne ; Volkmer, Hansjürgen ; Walmod, Peter S ; Berezin, Vladimir ; Bock, Elisabeth. / Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway. I: Journal of Neurochemistry. 2007 ; Bind 103, Nr. 4. s. 1396-407.

@article{d197e370acc511ddb538000ea68e967b,

title = "Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.",

abstract = "A combinatorial library of undecapeptides was produced and utilized for the isolation of peptide binding to the fibronectin type 3 modules (F3I-F3II) of the neural cell adhesion molecule (NCAM). The isolated peptides were sequenced and produced as dendrimers. Two of the peptides (denoted ENFIN2 and ENFIN11) were confirmed to bind to F3I-F3II of NCAM by surface plasmon resonance. The peptides induced neurite outgrowth in primary cerebellar neurons and PC12E2 cells, but had no apparent neuroprotective properties. NCAM is known to activate different intracellular pathways, including signaling through the fibroblast growth factor receptor, the Src-related non-receptor tyrosine kinase Fyn, and heterotrimeric G-proteins. Interestingly, neurite outgrowth stimulated by ENFIN2 and ENFIN11 was independent of signaling through fibroblast growth factor receptor and Fyn, but could be inhibited with pertussis toxin, an inhibitor of certain heterotrimeric G-proteins. Neurite outgrowth induced by trans-homophilic NCAM was unaffected by the peptides, whereas knockdown of NCAM completely abrogated ENFIN2- and ENFIN11-induced neuritogenesis. These observations suggest that ENFIN2 and ENFIN11 induce neurite outgrowth in an NCAM-dependent manner through G-protein-coupled signal transduction pathways. Thus, ENFIN2 and ENFIN11 may be valuable for exploring this particular type of NCAM-mediated signaling.",

author = "Hansen, {Raino Kristian} and Claus Christensen and Irina Korshunova and Martin Kriebel and Nadine Burkarth and Kiselyov, {Vladislav V} and Marianne Olsen and S{\o}ren Ostergaard and Arne Holm and Hansj{\"u}rgen Volkmer and Walmod, {Peter S} and Vladimir Berezin and Elisabeth Bock",

note = "Keywords: Animals; Cell Proliferation; Cells, Cultured; Cerebellum; Humans; Mice; Neural Cell Adhesion Molecules; Neurites; PC12 Cells; Peptides; Protein Binding; Rats; Rats, Wistar; Receptors, G-Protein-Coupled; Signal Transduction; Toxoids",

year = "2007",

doi = "10.1111/j.1471-4159.2007.04894.x",

language = "English",

volume = "103",

pages = "1396--407",

journal = "Journal of Neurochemistry",

issn = "0022-3042",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Identification of NCAM-binding peptides promoting neurite outgrowth via a heterotrimeric G-protein-coupled pathway.

AU - Hansen, Raino Kristian

AU - Christensen, Claus

AU - Korshunova, Irina

AU - Kriebel, Martin

AU - Burkarth, Nadine

AU - Kiselyov, Vladislav V

AU - Olsen, Marianne

AU - Ostergaard, Søren

AU - Holm, Arne

AU - Volkmer, Hansjürgen

AU - Walmod, Peter S

AU - Berezin, Vladimir

AU - Bock, Elisabeth

N1 - Keywords: Animals; Cell Proliferation; Cells, Cultured; Cerebellum; Humans; Mice; Neural Cell Adhesion Molecules; Neurites; PC12 Cells; Peptides; Protein Binding; Rats; Rats, Wistar; Receptors, G-Protein-Coupled; Signal Transduction; Toxoids

PY - 2007

Y1 - 2007

N2 - A combinatorial library of undecapeptides was produced and utilized for the isolation of peptide binding to the fibronectin type 3 modules (F3I-F3II) of the neural cell adhesion molecule (NCAM). The isolated peptides were sequenced and produced as dendrimers. Two of the peptides (denoted ENFIN2 and ENFIN11) were confirmed to bind to F3I-F3II of NCAM by surface plasmon resonance. The peptides induced neurite outgrowth in primary cerebellar neurons and PC12E2 cells, but had no apparent neuroprotective properties. NCAM is known to activate different intracellular pathways, including signaling through the fibroblast growth factor receptor, the Src-related non-receptor tyrosine kinase Fyn, and heterotrimeric G-proteins. Interestingly, neurite outgrowth stimulated by ENFIN2 and ENFIN11 was independent of signaling through fibroblast growth factor receptor and Fyn, but could be inhibited with pertussis toxin, an inhibitor of certain heterotrimeric G-proteins. Neurite outgrowth induced by trans-homophilic NCAM was unaffected by the peptides, whereas knockdown of NCAM completely abrogated ENFIN2- and ENFIN11-induced neuritogenesis. These observations suggest that ENFIN2 and ENFIN11 induce neurite outgrowth in an NCAM-dependent manner through G-protein-coupled signal transduction pathways. Thus, ENFIN2 and ENFIN11 may be valuable for exploring this particular type of NCAM-mediated signaling.

AB - A combinatorial library of undecapeptides was produced and utilized for the isolation of peptide binding to the fibronectin type 3 modules (F3I-F3II) of the neural cell adhesion molecule (NCAM). The isolated peptides were sequenced and produced as dendrimers. Two of the peptides (denoted ENFIN2 and ENFIN11) were confirmed to bind to F3I-F3II of NCAM by surface plasmon resonance. The peptides induced neurite outgrowth in primary cerebellar neurons and PC12E2 cells, but had no apparent neuroprotective properties. NCAM is known to activate different intracellular pathways, including signaling through the fibroblast growth factor receptor, the Src-related non-receptor tyrosine kinase Fyn, and heterotrimeric G-proteins. Interestingly, neurite outgrowth stimulated by ENFIN2 and ENFIN11 was independent of signaling through fibroblast growth factor receptor and Fyn, but could be inhibited with pertussis toxin, an inhibitor of certain heterotrimeric G-proteins. Neurite outgrowth induced by trans-homophilic NCAM was unaffected by the peptides, whereas knockdown of NCAM completely abrogated ENFIN2- and ENFIN11-induced neuritogenesis. These observations suggest that ENFIN2 and ENFIN11 induce neurite outgrowth in an NCAM-dependent manner through G-protein-coupled signal transduction pathways. Thus, ENFIN2 and ENFIN11 may be valuable for exploring this particular type of NCAM-mediated signaling.

U2 - 10.1111/j.1471-4159.2007.04894.x

DO - 10.1111/j.1471-4159.2007.04894.x

M3 - Journal article

C2 - 17854387

VL - 103

SP - 1396

EP - 1407

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 4

ER -