Identification of Rv0222 from RD4 as a novel serodiagnostic target for tuberculosis

Ida Rosenkrands; Claus Aagaard; Karin Weldingh; Inger Brock; Morten H Dziegiel; Mahavir Singh; Søren Hoff; Pernille Ravn; Peter Andersen

doi:http://dx.doi.org/10.1016/j.tube.2007.12.001

Identification of Rv0222 from RD4 as a novel serodiagnostic target for tuberculosis

Ida Rosenkrands, Claus Aagaard, Karin Weldingh, Inger Brock, Morten H Dziegiel, Mahavir Singh, Søren Hoff, Pernille Ravn, Peter Andersen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

32 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008-Jul

Originalsprog	Engelsk
Tidsskrift	Tuberculosis
Vol/bind	88
Udgave nummer	4
Sider (fra-til)	335-43
Antal sider	8
ISSN	1472-9792
DOI	https://doi.org/10.1016/j.tube.2007.12.001
Status	Udgivet - 2008

Adgang til dokumentet

http://dx.doi.org/10.1016/j.tube.2007.12.001

Citationsformater

Identification of Rv0222 from RD4 as a novel serodiagnostic target for tuberculosis. / Rosenkrands, Ida; Aagaard, Claus; Weldingh, Karin; Brock, Inger; Dziegiel, Morten H; Singh, Mahavir; Hoff, Søren; Ravn, Pernille; Andersen, Peter.

I: Tuberculosis, Bind 88, Nr. 4, 2008, s. 335-43.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{8eca9d8500d94086b16bdc2873de842a,

title = "Identification of Rv0222 from RD4 as a novel serodiagnostic target for tuberculosis",

abstract = "Forty-seven Mycobacterium tuberculosis genes from the 'regions of difference' RD2-7, RD9-13 and RD15 were cloned and expressed, and the purified recombinant proteins were screened for their serodiagnostic potential. Evaluation of six selected proteins in serum samples from Danish resident tuberculosis patients and healthy controls led to identification of Rv0222 as the most promising serodiagnostic antigen. Recognition of the Rv0222 was compared with the 38 kDa protein and a fusion protein of the RD1 proteins ESAT-6 and CFP10 in a serum panel from pulmonary tuberculosis (TB) patients from Uganda. The highest overall sensitivity was observed for Rv0222 compared to BCG-vaccinated non-endemic healthy controls as well as symptomatic endemic controls. Importantly, Rv0222 identified human immuno deficiency (HIV) virus-positive patients and HIV-negative patients with the same overall sensitivity. The results emphasize the importance of cut-off values in TB endemic regions based on endemic control individuals to diagnose active TB, and identify Rv0222 as a promising new antigen for serodiagnosis of TB in both HIV-negative and HIV-positive patients.",

author = "Ida Rosenkrands and Claus Aagaard and Karin Weldingh and Inger Brock and Dziegiel, {Morten H} and Mahavir Singh and S{\o}ren Hoff and Pernille Ravn and Peter Andersen",

year = "2008",

doi = "http://dx.doi.org/10.1016/j.tube.2007.12.001",

language = "English",

volume = "88",

pages = "335--43",

journal = "Tuberculosis",

issn = "1472-9792",

publisher = "Churchill Livingstone",

number = "4",

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TY - JOUR

T1 - Identification of Rv0222 from RD4 as a novel serodiagnostic target for tuberculosis

AU - Rosenkrands, Ida

AU - Aagaard, Claus

AU - Weldingh, Karin

AU - Brock, Inger

AU - Dziegiel, Morten H

AU - Singh, Mahavir

AU - Hoff, Søren

AU - Ravn, Pernille

AU - Andersen, Peter

PY - 2008

Y1 - 2008

N2 - Forty-seven Mycobacterium tuberculosis genes from the 'regions of difference' RD2-7, RD9-13 and RD15 were cloned and expressed, and the purified recombinant proteins were screened for their serodiagnostic potential. Evaluation of six selected proteins in serum samples from Danish resident tuberculosis patients and healthy controls led to identification of Rv0222 as the most promising serodiagnostic antigen. Recognition of the Rv0222 was compared with the 38 kDa protein and a fusion protein of the RD1 proteins ESAT-6 and CFP10 in a serum panel from pulmonary tuberculosis (TB) patients from Uganda. The highest overall sensitivity was observed for Rv0222 compared to BCG-vaccinated non-endemic healthy controls as well as symptomatic endemic controls. Importantly, Rv0222 identified human immuno deficiency (HIV) virus-positive patients and HIV-negative patients with the same overall sensitivity. The results emphasize the importance of cut-off values in TB endemic regions based on endemic control individuals to diagnose active TB, and identify Rv0222 as a promising new antigen for serodiagnosis of TB in both HIV-negative and HIV-positive patients.

AB - Forty-seven Mycobacterium tuberculosis genes from the 'regions of difference' RD2-7, RD9-13 and RD15 were cloned and expressed, and the purified recombinant proteins were screened for their serodiagnostic potential. Evaluation of six selected proteins in serum samples from Danish resident tuberculosis patients and healthy controls led to identification of Rv0222 as the most promising serodiagnostic antigen. Recognition of the Rv0222 was compared with the 38 kDa protein and a fusion protein of the RD1 proteins ESAT-6 and CFP10 in a serum panel from pulmonary tuberculosis (TB) patients from Uganda. The highest overall sensitivity was observed for Rv0222 compared to BCG-vaccinated non-endemic healthy controls as well as symptomatic endemic controls. Importantly, Rv0222 identified human immuno deficiency (HIV) virus-positive patients and HIV-negative patients with the same overall sensitivity. The results emphasize the importance of cut-off values in TB endemic regions based on endemic control individuals to diagnose active TB, and identify Rv0222 as a promising new antigen for serodiagnosis of TB in both HIV-negative and HIV-positive patients.

U2 - http://dx.doi.org/10.1016/j.tube.2007.12.001

DO - http://dx.doi.org/10.1016/j.tube.2007.12.001

M3 - Journal article

VL - 88

SP - 335

EP - 343

JO - Tuberculosis

JF - Tuberculosis

SN - 1472-9792

IS - 4

ER -