@article{b097bcf0e3ab11ddbf70000ea68e967b,
title = "Immunogold labelling is a quantitative method as demonstrated by studies on aminopeptidase N in microvillar membrane vesicles",
abstract = "Microvillar membrane vesicle preparations with varying content of aminopeptidase N were prepared from enterocytes of the pig small intestine. Postembedding immunogold labelling of aminopeptidase N was performed on these glutaraldehyde/paraformaldehyde-fixed, osmium tetroxide-treated and Epon-embedded microvillar membrane vesicles. The number of gold particles per micrometre microvillar membrane (labelling intensity) was calculated and compared to the corresponding enzymatic activity. A very close relationship was found between labelling intensity and aminopeptidase N activity, demonstrating that postembedding immunogold labelling can be used quantitatively.",
author = "Hansen, {Gert Helge} and Wetterberg, {L L} and H Sj{\"o}str{\"o}m and Ove Nor{\'e}n",
note = "Keywords: Aminopeptidases; Animals; Antigens, CD13; Immunohistochemistry; Intestine, Small; Microvilli; Papain; Subcellular Fractions; Swine",
year = "1992",
language = "English",
volume = "24",
pages = "132--6",
journal = "Journal of Molecular Histology",
issn = "1567-2379",
publisher = "Springer",
number = "3",
}