Immunohistochemical changes in the expression of HSP27 in exercised human vastus lateralis muscle

M Folkesson; Abigail Mackey; L Holm; M Kjaer; G Paulsen; T Raastad; J Henriksson; F Kadi

doi:10.1111/j.1748-1716.2008.01875.x

Immunohistochemical changes in the expression of HSP27 in exercised human vastus lateralis muscle

M Folkesson, Abigail Mackey, L Holm, M Kjaer, G Paulsen, T Raastad, J Henriksson, F Kadi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

23 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008-Nov

Originalsprog	Engelsk
Tidsskrift	Acta Physiologica (Print Edition)
Vol/bind	194
Udgave nummer	3
Sider (fra-til)	215-22
Antal sider	7
ISSN	1748-1708
DOI	https://doi.org/10.1111/j.1748-1716.2008.01875.x
Status	Udgivet - 2008

Bibliografisk note

Keywords: Adult; Bicycling; Exercise; HSP27 Heat-Shock Proteins; Humans; Male; Muscle Contraction; Muscle Fibers, Skeletal; Physical Endurance; Quadriceps Muscle; Young Adult

Adgang til dokumentet

10.1111/j.1748-1716.2008.01875.x

Citationsformater

@article{631f33a0dcc911de974b000ea68e967b,

title = "Immunohistochemical changes in the expression of HSP27 in exercised human vastus lateralis muscle",

abstract = "AIM: The role of HSP27 in the adaptive process of skeletal muscle to exercise, especially in humans, is not well understood. The objective of this study was to investigate immunohistochemical changes in HSP27 expression in human vastus lateralis muscle following resistance and endurance exercises. METHODS: Two different exercise protocols were used: (1) one-leg ergometer cycling (EC, n = 6) consisting of two 30-min bouts at 40% and 75% of peak oxygen uptake, respectively, and (2) leg extension resistance exercise (RE, n = 9) including 10 sets of eight repetitions at a load corresponding to 70% of one maximal repetition (1RM). Immunohistochemistry using specific monoclonal antibodies was used to determine the location of HSP27 protein in muscle biopsies from human vastus lateralis. RESULTS: Our results show that RE, but not EC, induced a significant appearance of scattered accumulations of HSP27 protein in muscle fibres from five of nine subjects. The number of fibres with accumulation of HSP27 in RE ranged from 0% to 32% with a mean of 6.3% of the total number of fibres. CONCLUSION: We conclude that this rapid HSP27 protein relocation after RE is an important player in the cellular remodelling of human muscle fibres in response to exercise involving high-force contractions, but not in response to endurance exercises.",

author = "M Folkesson and Abigail Mackey and L Holm and M Kjaer and G Paulsen and T Raastad and J Henriksson and F Kadi",

note = "Keywords: Adult; Bicycling; Exercise; HSP27 Heat-Shock Proteins; Humans; Male; Muscle Contraction; Muscle Fibers, Skeletal; Physical Endurance; Quadriceps Muscle; Young Adult",

year = "2008",

doi = "10.1111/j.1748-1716.2008.01875.x",

language = "English",

volume = "194",

pages = "215--22",

journal = "Acta Physiologica",

issn = "1748-1708",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Immunohistochemical changes in the expression of HSP27 in exercised human vastus lateralis muscle

AU - Folkesson, M

AU - Mackey, Abigail

AU - Holm, L

AU - Kjaer, M

AU - Paulsen, G

AU - Raastad, T

AU - Henriksson, J

AU - Kadi, F

N1 - Keywords: Adult; Bicycling; Exercise; HSP27 Heat-Shock Proteins; Humans; Male; Muscle Contraction; Muscle Fibers, Skeletal; Physical Endurance; Quadriceps Muscle; Young Adult

PY - 2008

Y1 - 2008

N2 - AIM: The role of HSP27 in the adaptive process of skeletal muscle to exercise, especially in humans, is not well understood. The objective of this study was to investigate immunohistochemical changes in HSP27 expression in human vastus lateralis muscle following resistance and endurance exercises. METHODS: Two different exercise protocols were used: (1) one-leg ergometer cycling (EC, n = 6) consisting of two 30-min bouts at 40% and 75% of peak oxygen uptake, respectively, and (2) leg extension resistance exercise (RE, n = 9) including 10 sets of eight repetitions at a load corresponding to 70% of one maximal repetition (1RM). Immunohistochemistry using specific monoclonal antibodies was used to determine the location of HSP27 protein in muscle biopsies from human vastus lateralis. RESULTS: Our results show that RE, but not EC, induced a significant appearance of scattered accumulations of HSP27 protein in muscle fibres from five of nine subjects. The number of fibres with accumulation of HSP27 in RE ranged from 0% to 32% with a mean of 6.3% of the total number of fibres. CONCLUSION: We conclude that this rapid HSP27 protein relocation after RE is an important player in the cellular remodelling of human muscle fibres in response to exercise involving high-force contractions, but not in response to endurance exercises.

AB - AIM: The role of HSP27 in the adaptive process of skeletal muscle to exercise, especially in humans, is not well understood. The objective of this study was to investigate immunohistochemical changes in HSP27 expression in human vastus lateralis muscle following resistance and endurance exercises. METHODS: Two different exercise protocols were used: (1) one-leg ergometer cycling (EC, n = 6) consisting of two 30-min bouts at 40% and 75% of peak oxygen uptake, respectively, and (2) leg extension resistance exercise (RE, n = 9) including 10 sets of eight repetitions at a load corresponding to 70% of one maximal repetition (1RM). Immunohistochemistry using specific monoclonal antibodies was used to determine the location of HSP27 protein in muscle biopsies from human vastus lateralis. RESULTS: Our results show that RE, but not EC, induced a significant appearance of scattered accumulations of HSP27 protein in muscle fibres from five of nine subjects. The number of fibres with accumulation of HSP27 in RE ranged from 0% to 32% with a mean of 6.3% of the total number of fibres. CONCLUSION: We conclude that this rapid HSP27 protein relocation after RE is an important player in the cellular remodelling of human muscle fibres in response to exercise involving high-force contractions, but not in response to endurance exercises.

U2 - 10.1111/j.1748-1716.2008.01875.x

DO - 10.1111/j.1748-1716.2008.01875.x

M3 - Journal article

C2 - 18489727

VL - 194

SP - 215

EP - 222

JO - Acta Physiologica

JF - Acta Physiologica

SN - 1748-1708

IS - 3

ER -