Abstract
Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.
Originalsprog | Engelsk |
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Titel | Methods in Molecular Biology : Targeting the Surface of Infected Erythrocytes |
Antal sider | 10 |
Forlag | Humana Press |
Publikationsdato | 2022 |
Sider | 69-78 |
ISBN (Elektronisk) | 978-1-0716-2189-9 |
DOI | |
Status | Udgivet - 2022 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2470 |
ISSN | 1064-3745 |
Bibliografisk note
Publisher Copyright:© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.