Immunomagnetic Selection of Plasmodium falciparum–Infected Erythrocytes Expressing Particular PfEMP1 Variants

Mary Lopez-Perez*, Rebecca Wendelboe Olsen

*Corresponding author af dette arbejde

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningpeer review

3 Citationer (Scopus)

Abstract

Plasmodium falciparum expresses a broad range of proteins on the surface of infected erythrocytes (IEs), including members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. This protocol describes an immunomagnetic selection method using PfEMP1-specific antibodies to obtain a parasite clone homogenously expressing a particular PfEMP1 protein. The expression of the corresponding PfEMP1 is later tested by flow cytometry, and the selected parasites can be used for further analysis.

OriginalsprogEngelsk
TitelMethods in Molecular Biology : Targeting the Surface of Infected Erythrocytes
Antal sider10
ForlagHumana Press
Publikationsdato2022
Sider69-78
ISBN (Elektronisk)978-1-0716-2189-9
DOI
StatusUdgivet - 2022
NavnMethods in Molecular Biology
Vol/bind2470
ISSN1064-3745

Bibliografisk note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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