IMP3 expression in human ovarian cancer is associated with improved survival

Aurelia Noske; Areeg Faggad; Ralph Wirtz; Silvia Darb-Esfahani; Jalid Sehouli; Bruno Sinn; Finn Cilius Nielsen; Wilko Weichert; Ann-Christin Buckendahl; Annika Röske; Berit Müller; Manfred Dietel; Carsten Denkert

doi:10.1097/PGP.0b013e318189a724

IMP3 expression in human ovarian cancer is associated with improved survival

Aurelia Noske, Areeg Faggad, Ralph Wirtz, Silvia Darb-Esfahani, Jalid Sehouli, Bruno Sinn, Finn Cilius Nielsen, Wilko Weichert, Ann-Christin Buckendahl, Annika Röske, Berit Müller, Manfred Dietel, Carsten Denkert

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

25 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-May

Originalsprog	Engelsk
Tidsskrift	International Journal of Gynecological Pathology
Vol/bind	28
Udgave nummer	3
Sider (fra-til)	203-10
Antal sider	7
ISSN	0277-1691
DOI	https://doi.org/10.1097/PGP.0b013e318189a724
Status	Udgivet - 2009

Bibliografisk note

Keywords: Adult; Aged; Aged, 80 and over; Blotting, Western; Female; Gene Expression; Humans; Kaplan-Meiers Estimate; Middle Aged; Neoplasm Proteins; Neoplasm Staging; Ovarian Neoplasms; Prognosis; RNA, Messenger; RNA-Binding Proteins; Reverse Transcriptase Polymerase Chain Reaction; Tumor Markers, Biological

Adgang til dokumentet

10.1097/PGP.0b013e318189a724

Citationsformater

Noske, A., Faggad, A., Wirtz, R., Darb-Esfahani, S., Sehouli, J., Sinn, B., Nielsen, F. C., Weichert, W., Buckendahl, A.-C., Röske, A., Müller, B., Dietel, M., & Denkert, C. (2009). IMP3 expression in human ovarian cancer is associated with improved survival. International Journal of Gynecological Pathology, 28(3), 203-10. https://doi.org/10.1097/PGP.0b013e318189a724

@article{d59e29f06a4e11df928f000ea68e967b,

title = "IMP3 expression in human ovarian cancer is associated with improved survival",

abstract = "The insulin-like growth factor-II mRNA-binding protein IMP3 plays an important role in embryogenesis and recent reports suggest an involvement in tumorigenesis. Although IMP3 expression has been well studied in mouse and human fetal and adult gonads, its role in ovarian cancer is unknown. We investigated the expression of IMP3 at protein and mRNA levels in a cohort of primary ovarian carcinomas and in 11 ovarian cancer cell lines. Western blot analysis revealed an expression of IMP3 in all ovarian cancer cell lines and immunohistochemistry demonstrated a positive cytoplasmic staining in 32 of 68 carcinomas (47%). In contrast, epithelium of borderline tumors, as well as, benign ovarian lesions and normal ovaries exhibited only weak or no IMP3 expression. In univariate Kaplan-Meier analysis, IMP3 protein expression was significantly associated with better overall survival (P=0.048). To confirm these findings, we further determined IMP3 mRNA expression in 43 ovarian cancer specimens by real time quantitative reverse transcription-polymerase chain reaction. A significant correlation between protein and mRNA levels (r=0.414, P=0.006), as well as a correlation of IMP3 mRNA expression with patient overall survival (P=0.044), was observed. Our results demonstrate that IMP3 is expressed in a subpopulation of ovarian cancer and a marker of good prognosis.",

author = "Aurelia Noske and Areeg Faggad and Ralph Wirtz and Silvia Darb-Esfahani and Jalid Sehouli and Bruno Sinn and Nielsen, {Finn Cilius} and Wilko Weichert and Ann-Christin Buckendahl and Annika R{\"o}ske and Berit M{\"u}ller and Manfred Dietel and Carsten Denkert",

note = "Keywords: Adult; Aged; Aged, 80 and over; Blotting, Western; Female; Gene Expression; Humans; Kaplan-Meiers Estimate; Middle Aged; Neoplasm Proteins; Neoplasm Staging; Ovarian Neoplasms; Prognosis; RNA, Messenger; RNA-Binding Proteins; Reverse Transcriptase Polymerase Chain Reaction; Tumor Markers, Biological",

year = "2009",

doi = "10.1097/PGP.0b013e318189a724",

language = "English",

volume = "28",

pages = "203--10",

journal = "International Journal of Gynecological Pathology",

issn = "0277-1691",

publisher = "Lippincott Williams & Wilkins",

number = "3",

}

TY - JOUR

T1 - IMP3 expression in human ovarian cancer is associated with improved survival

AU - Noske, Aurelia

AU - Faggad, Areeg

AU - Wirtz, Ralph

AU - Darb-Esfahani, Silvia

AU - Sehouli, Jalid

AU - Sinn, Bruno

AU - Nielsen, Finn Cilius

AU - Weichert, Wilko

AU - Buckendahl, Ann-Christin

AU - Röske, Annika

AU - Müller, Berit

AU - Dietel, Manfred

AU - Denkert, Carsten

N1 - Keywords: Adult; Aged; Aged, 80 and over; Blotting, Western; Female; Gene Expression; Humans; Kaplan-Meiers Estimate; Middle Aged; Neoplasm Proteins; Neoplasm Staging; Ovarian Neoplasms; Prognosis; RNA, Messenger; RNA-Binding Proteins; Reverse Transcriptase Polymerase Chain Reaction; Tumor Markers, Biological

PY - 2009

Y1 - 2009

N2 - The insulin-like growth factor-II mRNA-binding protein IMP3 plays an important role in embryogenesis and recent reports suggest an involvement in tumorigenesis. Although IMP3 expression has been well studied in mouse and human fetal and adult gonads, its role in ovarian cancer is unknown. We investigated the expression of IMP3 at protein and mRNA levels in a cohort of primary ovarian carcinomas and in 11 ovarian cancer cell lines. Western blot analysis revealed an expression of IMP3 in all ovarian cancer cell lines and immunohistochemistry demonstrated a positive cytoplasmic staining in 32 of 68 carcinomas (47%). In contrast, epithelium of borderline tumors, as well as, benign ovarian lesions and normal ovaries exhibited only weak or no IMP3 expression. In univariate Kaplan-Meier analysis, IMP3 protein expression was significantly associated with better overall survival (P=0.048). To confirm these findings, we further determined IMP3 mRNA expression in 43 ovarian cancer specimens by real time quantitative reverse transcription-polymerase chain reaction. A significant correlation between protein and mRNA levels (r=0.414, P=0.006), as well as a correlation of IMP3 mRNA expression with patient overall survival (P=0.044), was observed. Our results demonstrate that IMP3 is expressed in a subpopulation of ovarian cancer and a marker of good prognosis.

AB - The insulin-like growth factor-II mRNA-binding protein IMP3 plays an important role in embryogenesis and recent reports suggest an involvement in tumorigenesis. Although IMP3 expression has been well studied in mouse and human fetal and adult gonads, its role in ovarian cancer is unknown. We investigated the expression of IMP3 at protein and mRNA levels in a cohort of primary ovarian carcinomas and in 11 ovarian cancer cell lines. Western blot analysis revealed an expression of IMP3 in all ovarian cancer cell lines and immunohistochemistry demonstrated a positive cytoplasmic staining in 32 of 68 carcinomas (47%). In contrast, epithelium of borderline tumors, as well as, benign ovarian lesions and normal ovaries exhibited only weak or no IMP3 expression. In univariate Kaplan-Meier analysis, IMP3 protein expression was significantly associated with better overall survival (P=0.048). To confirm these findings, we further determined IMP3 mRNA expression in 43 ovarian cancer specimens by real time quantitative reverse transcription-polymerase chain reaction. A significant correlation between protein and mRNA levels (r=0.414, P=0.006), as well as a correlation of IMP3 mRNA expression with patient overall survival (P=0.044), was observed. Our results demonstrate that IMP3 is expressed in a subpopulation of ovarian cancer and a marker of good prognosis.

U2 - 10.1097/PGP.0b013e318189a724

DO - 10.1097/PGP.0b013e318189a724

M3 - Journal article

C2 - 19620937

SN - 0277-1691

VL - 28

SP - 203

EP - 210

JO - International Journal of Gynecological Pathology

JF - International Journal of Gynecological Pathology

IS - 3

ER -