Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters: Results of human intervention trials

Franziska Ferk; Miroslav Mišík; Armen Nersesyan; Christoph Pichler; Walter Jäger; Thomas Szekeres; Rodrig Marculescu; Henrik E Poulsen; Trine Henriksen; Roberto Bono; Valeria Romanazzi; Halh Al-Serori; Martin Biendl; Karl-Heinz Wagner; Michael Kundi; Siegfried Knasmüller

doi:10.1002/mnfr.201500355

Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters: Results of human intervention trials

Franziska Ferk, Miroslav Mišík, Armen Nersesyan, Christoph Pichler, Walter Jäger, Thomas Szekeres, Rodrig Marculescu, Henrik E Poulsen, Trine Henriksen, Roberto Bono, Valeria Romanazzi, Halh Al-Serori, Martin Biendl, Karl-Heinz Wagner, Michael Kundi, Siegfried Knasmüller

Institut for Klinisk Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

38 Citationer (Scopus)

Abstract

SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans.

METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention.

CONCLUSION: Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage.

Originalsprog	Engelsk
Tidsskrift	Molecular Nutrition & Food Research
Vol/bind	60
Udgave nummer	4
Sider (fra-til)	773-86
Antal sider	14
ISSN	1613-4125
DOI	https://doi.org/10.1002/mnfr.201500355
Status	Udgivet - apr. 2016

Adgang til dokumentet

10.1002/mnfr.201500355

Citationsformater

Ferk, F., Mišík, M., Nersesyan, A., Pichler, C., Jäger, W., Szekeres, T., Marculescu, R., Poulsen, H. E., Henriksen, T., Bono, R., Romanazzi, V., Al-Serori, H., Biendl, M., Wagner, K.-H., Kundi, M., & Knasmüller, S. (2016). Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters: Results of human intervention trials. Molecular Nutrition & Food Research, 60(4), 773-86. https://doi.org/10.1002/mnfr.201500355

Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters: Results of human intervention trials. / Ferk, Franziska; Mišík, Miroslav; Nersesyan, Armen et al.
I: Molecular Nutrition & Food Research, Bind 60, Nr. 4, 04.2016, s. 773-86.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Ferk, F, Mišík, M, Nersesyan, A, Pichler, C, Jäger, W, Szekeres, T, Marculescu, R, Poulsen, HE, Henriksen, T, Bono, R, Romanazzi, V, Al-Serori, H, Biendl, M, Wagner, K-H, Kundi, M & Knasmüller, S 2016, 'Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters: Results of human intervention trials', Molecular Nutrition & Food Research, bind 60, nr. 4, s. 773-86. https://doi.org/10.1002/mnfr.201500355

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title = "Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters: Results of human intervention trials",

abstract = "SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans.METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention.CONCLUSION: Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage.",

keywords = "Adult, DNA, DNA Damage, Deoxyguanosine, Female, Flavonoids, Humans, Male, Propiophenones, Protective Agents, Single-Cell Analysis, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't",

author = "Franziska Ferk and Miroslav Mi{\v s}{\'i}k and Armen Nersesyan and Christoph Pichler and Walter J{\"a}ger and Thomas Szekeres and Rodrig Marculescu and Poulsen, {Henrik E} and Trine Henriksen and Roberto Bono and Valeria Romanazzi and Halh Al-Serori and Martin Biendl and Karl-Heinz Wagner and Michael Kundi and Siegfried Knasm{\"u}ller",

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year = "2016",

month = apr,

doi = "10.1002/mnfr.201500355",

language = "English",

volume = "60",

pages = "773--86",

journal = "Molecular Nutrition & Food Research",

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TY - JOUR

T1 - Impact of xanthohumol (a prenylated flavonoid from hops) on DNA stability and other health-related biochemical parameters

T2 - Results of human intervention trials

AU - Ferk, Franziska

AU - Mišík, Miroslav

AU - Nersesyan, Armen

AU - Pichler, Christoph

AU - Jäger, Walter

AU - Szekeres, Thomas

AU - Marculescu, Rodrig

AU - Poulsen, Henrik E

AU - Henriksen, Trine

AU - Bono, Roberto

AU - Romanazzi, Valeria

AU - Al-Serori, Halh

AU - Biendl, Martin

AU - Wagner, Karl-Heinz

AU - Kundi, Michael

AU - Knasmüller, Siegfried

PY - 2016/4

Y1 - 2016/4

N2 - SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans.METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention.CONCLUSION: Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage.

AB - SCOPE: Xanthohumol (XN) is a hop flavonoid found in beers and refreshment drinks. Results of in vitro and animal studies indicate that it causes beneficial health effects due to DNA protective, anti-inflammatory, antioxidant, and phytoestrogenic properties. Aim of the present study was to find out if XN causes alterations of health-related parameters in humans.METHODS AND RESULTS: The effects of the flavonoid were investigated in a randomized crossover intervention trial (n = 22) in which the participants consumed a XN drink (12 mg XN/P/day). We monitored alterations of the DNA stability in single cell gel electrophoresis assays in lymphocytes and of several health-related biomarkers. A decrease of oxidatively damaged purines and protection toward reactive oxygen species induced DNA damage was found after the consumption of the beverage; also the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine and 8-oxo-guanosine in urine was reduced. The assumption that the flavonoid causes DNA protection was confirmed in a randomized follow-up study with pure XN (n = 10) with a parallel design. Other biochemical parameters reflecting the redox- and hormonal status and lipid- and glucose metabolism were not altered after the intervention.CONCLUSION: Taken together, our data indicate that low doses of XN protect humans against oxidative DNA damage.

KW - Adult

KW - DNA

KW - DNA Damage

KW - Deoxyguanosine

KW - Female

KW - Flavonoids

KW - Humans

KW - Male

KW - Propiophenones

KW - Protective Agents

KW - Single-Cell Analysis

KW - Journal Article

KW - Randomized Controlled Trial

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/mnfr.201500355

DO - 10.1002/mnfr.201500355

M3 - Journal article

C2 - 26840505

SN - 1613-4125

VL - 60

SP - 773

EP - 786

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

IS - 4

ER -