Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation.

Cathy Hargreaves; Jens Find; Cathie Reeves; Keiko Gough; Josekutty Puthiyaparambil; Dolina Skudder; Susan van der Maas; Margaret Sigley; Mike Menzies; Charlie Low; Tim Mullin

doi:10.1139/X09-082

Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation.

Cathy Hargreaves, Jens Find, Cathie Reeves, Keiko Gough, Josekutty Puthiyaparambil, Dolina Skudder, Susan van der Maas, Margaret Sigley, Mike Menzies, Charlie Low, Tim Mullin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

47 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009

Originalsprog	Engelsk
Tidsskrift	Canadian Journal of Forest Research
Udgave nummer	39
Sider (fra-til)	1566-1574
Antal sider	9
ISSN	0045-5067
DOI	https://doi.org/10.1139/X09-082
Status	Udgivet - 2009

Bibliografisk note

Paper id:: 10.1139/x09-082

Adgang til dokumentet

10.1139/X09-082

Citationsformater

Hargreaves, C., Find, J., Reeves, C., Gough, K., Puthiyaparambil, J., Skudder, D., van der Maas, S., Sigley, M., Menzies, M., Low, C., & Mullin, T. (2009). Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation. Canadian Journal of Forest Research, (39), 1566-1574. https://doi.org/10.1139/X09-082

Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation. / Hargreaves, Cathy; Find, Jens; Reeves, Cathie et al.
I: Canadian Journal of Forest Research, Nr. 39, 2009, s. 1566-1574.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Hargreaves, C, Find, J, Reeves, C, Gough, K, Puthiyaparambil, J, Skudder, D, van der Maas, S, Sigley, M, Menzies, M, Low, C & Mullin, T 2009, 'Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation.', Canadian Journal of Forest Research, nr. 39, s. 1566-1574. https://doi.org/10.1139/X09-082

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title = "Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation.",

abstract = "The principal aim of this investigation was to improve somatic embryogenesis initiation and to enhance representation of families and genotypes within those families of Pinus radiata D. Don. A total of 19 open-pollinated seed families, many with unrelated and weakly related parents, were tested. Optimum stage of cone maturity for initiation success was tested by five collections made at 1 week intervals, spanning the developmental period from pro-embryo to cotyledonary embryos. Two media were compared; embryo-development media (EDM6) and a modified Litvay medium (Glitz). Two zygotic embryo explant-preparation techniques were tested; embryos with retained megagametophytes and excised embryos. Proliferating embryogenic tissues were obtained from all four treatments (2850 explants per treatment, 570 per collection time) for the 19 families. The best initiation rates were achieved with a combination of Glitz medium with excised zygotic embryos, with 55% of explants from all collections and all families combined giving rise to proliferating embryogenic tissue. At the optimal collection time for each of the families, this treatment gave a range of 47%-97% initiation success with an average of 70% per family.",

author = "Cathy Hargreaves and Jens Find and Cathie Reeves and Keiko Gough and Josekutty Puthiyaparambil and Dolina Skudder and {van der Maas}, Susan and Margaret Sigley and Mike Menzies and Charlie Low and Tim Mullin",

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T1 - Improving initiation, genotype capture, and family representation in somatic embryogenesis of Pinus radiata by a combination of zygotic embryo maturity, media, and explant preparation.

AU - Hargreaves, Cathy

AU - Find, Jens

AU - Reeves, Cathie

AU - Gough, Keiko

AU - Puthiyaparambil, Josekutty

AU - Skudder, Dolina

AU - van der Maas, Susan

AU - Sigley, Margaret

AU - Menzies, Mike

AU - Low, Charlie

AU - Mullin, Tim

N1 - Paper id:: 10.1139/x09-082

PY - 2009

Y1 - 2009

N2 - The principal aim of this investigation was to improve somatic embryogenesis initiation and to enhance representation of families and genotypes within those families of Pinus radiata D. Don. A total of 19 open-pollinated seed families, many with unrelated and weakly related parents, were tested. Optimum stage of cone maturity for initiation success was tested by five collections made at 1 week intervals, spanning the developmental period from pro-embryo to cotyledonary embryos. Two media were compared; embryo-development media (EDM6) and a modified Litvay medium (Glitz). Two zygotic embryo explant-preparation techniques were tested; embryos with retained megagametophytes and excised embryos. Proliferating embryogenic tissues were obtained from all four treatments (2850 explants per treatment, 570 per collection time) for the 19 families. The best initiation rates were achieved with a combination of Glitz medium with excised zygotic embryos, with 55% of explants from all collections and all families combined giving rise to proliferating embryogenic tissue. At the optimal collection time for each of the families, this treatment gave a range of 47%-97% initiation success with an average of 70% per family.

AB - The principal aim of this investigation was to improve somatic embryogenesis initiation and to enhance representation of families and genotypes within those families of Pinus radiata D. Don. A total of 19 open-pollinated seed families, many with unrelated and weakly related parents, were tested. Optimum stage of cone maturity for initiation success was tested by five collections made at 1 week intervals, spanning the developmental period from pro-embryo to cotyledonary embryos. Two media were compared; embryo-development media (EDM6) and a modified Litvay medium (Glitz). Two zygotic embryo explant-preparation techniques were tested; embryos with retained megagametophytes and excised embryos. Proliferating embryogenic tissues were obtained from all four treatments (2850 explants per treatment, 570 per collection time) for the 19 families. The best initiation rates were achieved with a combination of Glitz medium with excised zygotic embryos, with 55% of explants from all collections and all families combined giving rise to proliferating embryogenic tissue. At the optimal collection time for each of the families, this treatment gave a range of 47%-97% initiation success with an average of 70% per family.

U2 - 10.1139/X09-082

DO - 10.1139/X09-082

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SN - 0045-5067

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JO - Canadian Journal of Forest Research

JF - Canadian Journal of Forest Research

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ER -