In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

Jean Y Perentes; Trevor D McKee; Carsten D Ley; Hannah Mathiew; Michelle Dawson; Timothy P Padera; Lance L Munn; Rakesh K Jain; Yves Boucher

doi:10.1038/nmeth.1295

In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts

Jean Y Perentes, Trevor D McKee, Carsten D Ley, Hannah Mathiew, Michelle Dawson, Timothy P Padera, Lance L Munn, Rakesh K Jain, Yves Boucher

Undervisning

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

108 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-Feb

Originalsprog	Engelsk
Tidsskrift	Nature Methods
Vol/bind	6
Udgave nummer	2
Sider (fra-til)	143-5
Antal sider	2
ISSN	1548-7091
DOI	https://doi.org/10.1038/nmeth.1295
Status	Udgivet - 2009

Bibliografisk note

Keywords: Animals; Cell Line, Tumor; Cell Movement; Collagen; Extracellular Matrix; Fibroblasts; Humans; Image Enhancement; Integrin beta Chains; Mice; Mice, Transgenic; Microscopy, Confocal; Relaxin; Soft Tissue Neoplasms; Stromal Cells

Adgang til dokumentet

10.1038/nmeth.1295

Citationsformater

In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts. / Perentes, Jean Y; McKee, Trevor D; Ley, Carsten D; Mathiew, Hannah; Dawson, Michelle; Padera, Timothy P; Munn, Lance L; Jain, Rakesh K; Boucher, Yves.

I: Nature Methods, Bind 6, Nr. 2, 2009, s. 143-5.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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abstract = "Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.",

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AU - Perentes, Jean Y

AU - McKee, Trevor D

AU - Ley, Carsten D

AU - Mathiew, Hannah

AU - Dawson, Michelle

AU - Padera, Timothy P

AU - Munn, Lance L

AU - Jain, Rakesh K

AU - Boucher, Yves

N1 - Keywords: Animals; Cell Line, Tumor; Cell Movement; Collagen; Extracellular Matrix; Fibroblasts; Humans; Image Enhancement; Integrin beta Chains; Mice; Mice, Transgenic; Microscopy, Confocal; Relaxin; Soft Tissue Neoplasms; Stromal Cells

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N2 - Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

AB - Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases.

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DO - 10.1038/nmeth.1295

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