Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals

Søren Tvorup Christensen; H Quie; K Kemp; L. Rasmussen

doi:10.1006/cbir.1996.0055

Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals

Søren Tvorup Christensen, H Quie, K Kemp, L. Rasmussen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

30 Citationer (Scopus)

Abstract

Udgivelsesdato: 1996-Jun

Originalsprog	Engelsk
Tidsskrift	Cell Biology International
Vol/bind	20
Udgave nummer	6
Sider (fra-til)	437-44
Antal sider	7
ISSN	1065-6995
DOI	https://doi.org/10.1006/cbir.1996.0055
Status	Udgivet - 1996
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Animals; Cattle; Cell Division; Culture Media; Dose-Response Relationship, Drug; Hemin; Humans; Insulin; Recombinant Proteins; Signal Transduction; Tetrahymena thermophila

Adgang til dokumentet

10.1006/cbir.1996.0055

Citationsformater

Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals. / Christensen, Søren Tvorup; Quie, H; Kemp, K; Rasmussen, L.

I: Cell Biology International, Bind 20, Nr. 6, 1996, s. 437-44.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{f43483700fd011de8478000ea68e967b,

title = "Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals",

abstract = "Cells of Tetrahymena may produce autocrine signal molecules with effects on survival and proliferation. Here we have tested the effects of human recombinant and bovine insulin, and the B22-B30 fragment of bovine insulin over a wide range of concentrations (10(-5)-10(-18) M) on cell survival and proliferation in a synthetic nutrient medium. The cells were grown in conical flasks at low initial cell densities (40 and 400 cells/ml). Insulin prevented rapid cell death and/or promoted cell proliferation over two separate concentration ranges: down to nanomolar levels and again in the low pico- and femtomolar range. At an initial population density of 400 cells/ml the cells multiplied at both concentration intervals. At 40 or fewer organisms/ml the cells multiplied in the high concentration interval, whereas in the low interval they survived for about four times longer than those in the control cultures. B22-B30 added to cultures of 40 initial cells/ml produced a stimulation of cell survival in the low pico- and high femtomolar range. In the presence of hemin (50 nM) cells at 400 initial organisms/ml multiplied at insulin concentrations down to about 3 nM and again from 300 am to 10 pM. In some cases, hemin plus insulin activated cell proliferation between the two concentration intervals as well. At 40 cells/ml the cells not only survived but proliferated in the femtomolar range. Cells in cultures supplemented with both hemin and B22-B30 multiplied at the low concentration interval (from about 100 fM to 10 pM).",

author = "Christensen, {S{\o}ren Tvorup} and H Quie and K Kemp and L. Rasmussen",

note = "Keywords: Animals; Cattle; Cell Division; Culture Media; Dose-Response Relationship, Drug; Hemin; Humans; Insulin; Recombinant Proteins; Signal Transduction; Tetrahymena thermophila",

year = "1996",

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T1 - Insulin produces a biphasic response in Tetrahymena thermophila by stimulating cell survival and activating proliferation in two separate concentration intervals

AU - Christensen, Søren Tvorup

AU - Quie, H

AU - Kemp, K

AU - Rasmussen, L.

N1 - Keywords: Animals; Cattle; Cell Division; Culture Media; Dose-Response Relationship, Drug; Hemin; Humans; Insulin; Recombinant Proteins; Signal Transduction; Tetrahymena thermophila

PY - 1996

Y1 - 1996

N2 - Cells of Tetrahymena may produce autocrine signal molecules with effects on survival and proliferation. Here we have tested the effects of human recombinant and bovine insulin, and the B22-B30 fragment of bovine insulin over a wide range of concentrations (10(-5)-10(-18) M) on cell survival and proliferation in a synthetic nutrient medium. The cells were grown in conical flasks at low initial cell densities (40 and 400 cells/ml). Insulin prevented rapid cell death and/or promoted cell proliferation over two separate concentration ranges: down to nanomolar levels and again in the low pico- and femtomolar range. At an initial population density of 400 cells/ml the cells multiplied at both concentration intervals. At 40 or fewer organisms/ml the cells multiplied in the high concentration interval, whereas in the low interval they survived for about four times longer than those in the control cultures. B22-B30 added to cultures of 40 initial cells/ml produced a stimulation of cell survival in the low pico- and high femtomolar range. In the presence of hemin (50 nM) cells at 400 initial organisms/ml multiplied at insulin concentrations down to about 3 nM and again from 300 am to 10 pM. In some cases, hemin plus insulin activated cell proliferation between the two concentration intervals as well. At 40 cells/ml the cells not only survived but proliferated in the femtomolar range. Cells in cultures supplemented with both hemin and B22-B30 multiplied at the low concentration interval (from about 100 fM to 10 pM).

AB - Cells of Tetrahymena may produce autocrine signal molecules with effects on survival and proliferation. Here we have tested the effects of human recombinant and bovine insulin, and the B22-B30 fragment of bovine insulin over a wide range of concentrations (10(-5)-10(-18) M) on cell survival and proliferation in a synthetic nutrient medium. The cells were grown in conical flasks at low initial cell densities (40 and 400 cells/ml). Insulin prevented rapid cell death and/or promoted cell proliferation over two separate concentration ranges: down to nanomolar levels and again in the low pico- and femtomolar range. At an initial population density of 400 cells/ml the cells multiplied at both concentration intervals. At 40 or fewer organisms/ml the cells multiplied in the high concentration interval, whereas in the low interval they survived for about four times longer than those in the control cultures. B22-B30 added to cultures of 40 initial cells/ml produced a stimulation of cell survival in the low pico- and high femtomolar range. In the presence of hemin (50 nM) cells at 400 initial organisms/ml multiplied at insulin concentrations down to about 3 nM and again from 300 am to 10 pM. In some cases, hemin plus insulin activated cell proliferation between the two concentration intervals as well. At 40 cells/ml the cells not only survived but proliferated in the femtomolar range. Cells in cultures supplemented with both hemin and B22-B30 multiplied at the low concentration interval (from about 100 fM to 10 pM).

U2 - 10.1006/cbir.1996.0055

DO - 10.1006/cbir.1996.0055

M3 - Journal article

C2 - 8858828

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EP - 444

JO - Cell Biology International

JF - Cell Biology International

SN - 1065-6995

IS - 6

ER -