Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | European Journal of Endocrinology |
| Vol/bind | 157 |
| Udgave nummer | 4 |
| Sider (fra-til) | 465-71 |
| Antal sider | 6 |
| ISSN | 0804-4643 |
| DOI | |
| Status | Udgivet - 2007 |
Bibliografisk note
Keywords: Adipocytes; Adipose Tissue; Adult; Cells, Cultured; Cross-Sectional Studies; Diet Therapy; Exercise Therapy; Female; Humans; Inflammation Mediators; Insulin Resistance; Interleukin-18; Male; Obesity; Stromal Cells; Thinness; Weight LossAdgang til dokumentet
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I: European Journal of Endocrinology, Bind 157, Nr. 4, 2007, s. 465-71.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Interleukin-18 in plasma and adipose tissue: effects of obesity, insulin resistance, and weight loss
AU - Bruun, Jens M
AU - Stallknecht, Bente
AU - Helge, Jørn W
AU - Richelsen, Bjørn
N1 - Keywords: Adipocytes; Adipose Tissue; Adult; Cells, Cultured; Cross-Sectional Studies; Diet Therapy; Exercise Therapy; Female; Humans; Inflammation Mediators; Insulin Resistance; Interleukin-18; Male; Obesity; Stromal Cells; Thinness; Weight Loss
PY - 2007
Y1 - 2007
N2 - OBJECTIVE: Interleukin (IL)-18 is associated with obesity, insulin resistance, and cardiovascular disease. The present study compared 1) IL-18 in adipocytes versus stromal vascular (SV) cells, 2) IL-18 in plasma and adipose tissue (AT) in obese versus lean subjects, and 3) IL-18 in plasma, AT, and skeletal muscle (SM) in obese subjects after weight loss. SUBJECTS AND METHODS: At baseline, plasma and AT IL-18 in 23 obese subjects were compared with that in 12 lean subjects. The obese subjects were submitted to a 15-week life-style intervention (hypocaloric diet and daily exercise) after which plasma samples, AT, and SM biopsies were obtained. Analyses were performed by ELISA and RT-PCR respectively. RESULTS: IL-18 expression in isolated adipocytes was approximately 2% of that in SV cells. Plasma IL-18 was higher in obese subjects (P < 0.001) and associated with insulin resistance (HOMA; P < 0.001). AT expression of IL-18, CD14, and CD68 was higher in obese (P < 0.01). The intervention reduced body weight (P < 0.001), plasma IL-18 (P < 0.001), and increased insulin sensitivity (HOMA; P < 0.05). AT and SM expression of IL-18 remained unchanged after the intervention. Changes in plasma IL-18 were associated with changes in insulin sensitivity (P < 0.05) but not with BMI or AT expression of IL-18. CONCLUSION: Plasma IL-18 is associated with changes in insulin resistance and reduced after weight loss. AT expression of IL-18 is increased in obesity but not affected by weight loss, indicating that changes in plasma IL-18 are related to insulin resistance rather than changes in obesity per se.
AB - OBJECTIVE: Interleukin (IL)-18 is associated with obesity, insulin resistance, and cardiovascular disease. The present study compared 1) IL-18 in adipocytes versus stromal vascular (SV) cells, 2) IL-18 in plasma and adipose tissue (AT) in obese versus lean subjects, and 3) IL-18 in plasma, AT, and skeletal muscle (SM) in obese subjects after weight loss. SUBJECTS AND METHODS: At baseline, plasma and AT IL-18 in 23 obese subjects were compared with that in 12 lean subjects. The obese subjects were submitted to a 15-week life-style intervention (hypocaloric diet and daily exercise) after which plasma samples, AT, and SM biopsies were obtained. Analyses were performed by ELISA and RT-PCR respectively. RESULTS: IL-18 expression in isolated adipocytes was approximately 2% of that in SV cells. Plasma IL-18 was higher in obese subjects (P < 0.001) and associated with insulin resistance (HOMA; P < 0.001). AT expression of IL-18, CD14, and CD68 was higher in obese (P < 0.01). The intervention reduced body weight (P < 0.001), plasma IL-18 (P < 0.001), and increased insulin sensitivity (HOMA; P < 0.05). AT and SM expression of IL-18 remained unchanged after the intervention. Changes in plasma IL-18 were associated with changes in insulin sensitivity (P < 0.05) but not with BMI or AT expression of IL-18. CONCLUSION: Plasma IL-18 is associated with changes in insulin resistance and reduced after weight loss. AT expression of IL-18 is increased in obesity but not affected by weight loss, indicating that changes in plasma IL-18 are related to insulin resistance rather than changes in obesity per se.
U2 - 10.1530/EJE-07-0206
DO - 10.1530/EJE-07-0206
M3 - Journal article
C2 - 17893261
SN - 0804-4643
VL - 157
SP - 465
EP - 471
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
IS - 4
ER -