Interleukin-6 production in contracting human skeletal muscle is influenced by pre-exercise muscle glycogen content

A Steensberg; M A Febbraio; T Osada; P Schjerling; Gerrit Van Hall; B Saltin; B K Pedersen

Interleukin-6 production in contracting human skeletal muscle is influenced by pre-exercise muscle glycogen content

A Steensberg, M A Febbraio, T Osada, P Schjerling, Gerrit Van Hall, B Saltin, B K Pedersen

Exercise and Muscle

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

387 Citationer (Scopus)

Abstract

Udgivelsesdato: 2001-Dec-1

Originalsprog	Engelsk
Tidsskrift	Journal of Physiology
Vol/bind	537
Udgave nummer	Pt 2
Sider (fra-til)	633-9
Antal sider	6
ISSN	0022-3751
Status	Udgivet - 2001

Bibliografisk note

Keywords: Adult; Biological Availability; Exercise; Glycogen; Humans; Interleukin-6; Knee; Male; Muscle Contraction; Muscle, Skeletal

Citationsformater

@article{9d5d0a404f7211de87b8000ea68e967b,

title = "Interleukin-6 production in contracting human skeletal muscle is influenced by pre-exercise muscle glycogen content",

abstract = "1. Prolonged exercise results in a progressive decline in glycogen content and a concomitant increase in the release of the cytokine interleukin-6 (IL-6) from contracting muscle. This study tests the hypothesis that the exercise-induced IL-6 release from contracting muscle is linked to the intramuscular glycogen availability. 2. Seven men performed 5 h of a two-legged knee-extensor exercise, with one leg with normal, and one leg with reduced, muscle glycogen content. Muscle biopsies were obtained before (pre-ex), immediately after (end-ex) and 3 h into recovery (3 h rec) from exercise in both legs. In addition, catheters were placed in one femoral artery and both femoral veins and blood was sampled from these catheters prior to exercise and at 1 h intervals during exercise and into recovery. 3. Pre-exercise glycogen content was lower in the glycogen-depleted leg compared with the control leg. Intramuscular IL-6 mRNA levels increased with exercise in both legs, but this increase was augmented in the leg having the lowest glycogen content at end-ex. The arterial plasma concentration of IL-6 increased from 0.6 +/- 0.1 ng x l(-1) pre-ex to 21.7 +/- 5.6 ng x l(-1) end-ex. The depleted leg had already released IL-6 after 1 h (4.38 +/- 2.80 ng x min(-1) (P < 0.05)), whereas no significant release was observed in the control leg (0.36 +/- 0.14 ng x min(-1)). A significant net IL-6 release was not observed until 2 h in the control leg. 4. This study demonstrates that glycogen availability is associated with alterations in the rate of IL-6 production and release in contracting skeletal muscle.",

author = "A Steensberg and Febbraio, {M A} and T Osada and P Schjerling and {Van Hall}, Gerrit and B Saltin and Pedersen, {B K}",

note = "Keywords: Adult; Biological Availability; Exercise; Glycogen; Humans; Interleukin-6; Knee; Male; Muscle Contraction; Muscle, Skeletal",

year = "2001",

language = "English",

volume = "537",

pages = "633--9",

journal = "Journal of Physiology",

issn = "0022-3751",

publisher = "Wiley-Blackwell",

number = "Pt 2",

}

TY - JOUR

T1 - Interleukin-6 production in contracting human skeletal muscle is influenced by pre-exercise muscle glycogen content

AU - Steensberg, A

AU - Febbraio, M A

AU - Osada, T

AU - Schjerling, P

AU - Van Hall, Gerrit

AU - Saltin, B

AU - Pedersen, B K

N1 - Keywords: Adult; Biological Availability; Exercise; Glycogen; Humans; Interleukin-6; Knee; Male; Muscle Contraction; Muscle, Skeletal

PY - 2001

Y1 - 2001

N2 - 1. Prolonged exercise results in a progressive decline in glycogen content and a concomitant increase in the release of the cytokine interleukin-6 (IL-6) from contracting muscle. This study tests the hypothesis that the exercise-induced IL-6 release from contracting muscle is linked to the intramuscular glycogen availability. 2. Seven men performed 5 h of a two-legged knee-extensor exercise, with one leg with normal, and one leg with reduced, muscle glycogen content. Muscle biopsies were obtained before (pre-ex), immediately after (end-ex) and 3 h into recovery (3 h rec) from exercise in both legs. In addition, catheters were placed in one femoral artery and both femoral veins and blood was sampled from these catheters prior to exercise and at 1 h intervals during exercise and into recovery. 3. Pre-exercise glycogen content was lower in the glycogen-depleted leg compared with the control leg. Intramuscular IL-6 mRNA levels increased with exercise in both legs, but this increase was augmented in the leg having the lowest glycogen content at end-ex. The arterial plasma concentration of IL-6 increased from 0.6 +/- 0.1 ng x l(-1) pre-ex to 21.7 +/- 5.6 ng x l(-1) end-ex. The depleted leg had already released IL-6 after 1 h (4.38 +/- 2.80 ng x min(-1) (P < 0.05)), whereas no significant release was observed in the control leg (0.36 +/- 0.14 ng x min(-1)). A significant net IL-6 release was not observed until 2 h in the control leg. 4. This study demonstrates that glycogen availability is associated with alterations in the rate of IL-6 production and release in contracting skeletal muscle.

AB - 1. Prolonged exercise results in a progressive decline in glycogen content and a concomitant increase in the release of the cytokine interleukin-6 (IL-6) from contracting muscle. This study tests the hypothesis that the exercise-induced IL-6 release from contracting muscle is linked to the intramuscular glycogen availability. 2. Seven men performed 5 h of a two-legged knee-extensor exercise, with one leg with normal, and one leg with reduced, muscle glycogen content. Muscle biopsies were obtained before (pre-ex), immediately after (end-ex) and 3 h into recovery (3 h rec) from exercise in both legs. In addition, catheters were placed in one femoral artery and both femoral veins and blood was sampled from these catheters prior to exercise and at 1 h intervals during exercise and into recovery. 3. Pre-exercise glycogen content was lower in the glycogen-depleted leg compared with the control leg. Intramuscular IL-6 mRNA levels increased with exercise in both legs, but this increase was augmented in the leg having the lowest glycogen content at end-ex. The arterial plasma concentration of IL-6 increased from 0.6 +/- 0.1 ng x l(-1) pre-ex to 21.7 +/- 5.6 ng x l(-1) end-ex. The depleted leg had already released IL-6 after 1 h (4.38 +/- 2.80 ng x min(-1) (P < 0.05)), whereas no significant release was observed in the control leg (0.36 +/- 0.14 ng x min(-1)). A significant net IL-6 release was not observed until 2 h in the control leg. 4. This study demonstrates that glycogen availability is associated with alterations in the rate of IL-6 production and release in contracting skeletal muscle.

M3 - Journal article

C2 - 11731593

SN - 0022-3751

VL - 537

SP - 633

EP - 639

JO - Journal of Physiology

JF - Journal of Physiology

IS - Pt 2

ER -