Intramural Purkinje fibers facilitate rapid ventricular activation in the equine heart

Vibeke Sødring Elbrønd, Morten B Thomsen, Jonas L Isaksen, Ester Danielsen Lunde, Stefano Vincenti, Tobias Wang, Jørgen Tranum-Jensen, Kirstine Calloe*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

3 Citationer (Scopus)
49 Downloads (Pure)

Abstract

UNLABELLED: The Purkinje fibers convey the electrical impulses at much higher speed than the working myocardial cells. Thus, the distribution of the Purkinje network is of paramount importance for the timing and coordination of ventricular activation. The Purkinje fibers are found in the subendocardium of all species of mammals, but some mammals also possess an intramural Purkinje fiber network that provides for relatively instantaneous, burst-like activation of the entire ventricular wall, and gives rise to an rS configuration in lead II of the ECG.

AIM: To relate the topography of the horse heart and the distribution and histology of the conduction system to the pattern of ventricular activation as a mechanism for the unique electrical axis of the equine heart.

METHODS: The morphology and distribution of the cardiac conduction system was determined by histochemistry. The electrical activity was measured using ECG in the Einthoven and orthogonal configuration.

RESULTS: The long axis of the equine heart is close to vertical. Outside the nodal regions the conduction system consisted of Purkinje fibers connected by connexin 43 and long, slender parallel running transitional cells. The Purkinje fiber network extended deep into the ventricular walls. ECGs recorded in an orthogonal configuration revealed a mean electrical axis pointing in a cranial-to-left direction indicating ventricular activation in an apex-to-base direction.

CONCLUSION: The direction of the mean electrical axis in the equine heart is determined by the architecture of the intramural Purkinje network, rather than being a reflection of ventricular mass.

OriginalsprogEngelsk
Artikelnummere13925
TidsskriftActa Physiologica
Vol/bind237
Udgave nummer3
ISSN1748-1708
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
We thank the horse owners for providing access to horses for ECG measurements and for donating horse to the teaching and research program at the Vet School at the University of Copenhagen. Betina Gjedsted Andersen, Elisabeth Wairimu Petersen, Jan Lykke Jensen, and Vibeke Bøgelund Hansen are thanked for excellent technical assistance. Veterinarians Emma Smedsgaard Byskov and Camilla Rensch Davidsen are thanked for their contribution to establishing the whole mount PAS staining method. Dr Adrian Harrison, University of Copenhagen is thanked for custom‐made electrodes. The study was supported by the Carlsberg Foundation (CF19‐0431).

Funding Information:
We thank the horse owners for providing access to horses for ECG measurements and for donating horse to the teaching and research program at the Vet School at the University of Copenhagen. Betina Gjedsted Andersen, Elisabeth Wairimu Petersen, Jan Lykke Jensen, and Vibeke Bøgelund Hansen are thanked for excellent technical assistance. Veterinarians Emma Smedsgaard Byskov and Camilla Rensch Davidsen are thanked for their contribution to establishing the whole mount PAS staining method. Dr Adrian Harrison, University of Copenhagen is thanked for custom-made electrodes. The study was supported by the Carlsberg Foundation (CF19-0431).

Publisher Copyright:
© 2023 The Authors. Acta Physiologica published by John Wiley & Sons Ltd on behalf of Scandinavian Physiological Society.

Citationsformater