@inproceedings{d3b3c46e5e0647818dc6959bb3decf2f,
title = "Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)",
abstract = "Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78\%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025\% NaOH, an average molecular weight of 9500 was obtained at a yield of 30\%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.",
author = "H. St{\aa}lbrand and J. Lundqvist and A. Andersson and P. H{\"a}gglund and L. Anderson and F. Tjerneld and A. Jacobs and A. Teleman and O. Dahlman and M. Palm and G. Zacchi",
year = "2004",
month = dec,
day = "1",
language = "English",
isbn = "9780841238428",
series = "A C S Symposium Series",
publisher = "American Chemical Society",
pages = "66--78",
editor = "Paul Gatenholm and Maija Tenkanen",
booktitle = "Henicelluloses",
}