@article{986e132008bf11de8478000ea68e967b,
title = "Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens",
abstract = "Poly-N-acetyllactosamines (pLNs) are common terminal sugars of many N- and O-linked glycan structures present in glycoproteins and glycolipids. Utilizing various glycosyltransferases, we developed new and efficient chemoenzymatic methods for the synthesis of pLNs in gram-scale. Specifically, the use of sialyltransferases and fucosyltransferases enabled us to synthesize and purify 24 blood group and tumor-associated pLN derivatives with alpha-(2-->3)- and alpha-(2-->6)-linked sialic acid, as well as with alpha-(1-->2)- and alpha-(1-->3)-linked fucose. All synthesized derivatives were linked to a short 2-azidoethyl spacer for further modification.",
author = "Daniela Vasiliu and Nahid Razi and Yingning Zhang and Nathan Jacobsen and Kirk Allin and Xiaofei Liu and Julia Hoffmann and Ognian Bohorov and Ola Blixt",
note = "Keywords: Antigens, Neoplasm; Blood Group Antigens; Fucosyltransferases; Polysaccharides; Sialyltransferases",
year = "2006",
doi = "10.1016/j.carres.2006.03.043",
language = "English",
volume = "341",
pages = "1447--57",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Pergamon Press",
number = "10",
}