Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens

Daniela Vasiliu; Nahid Razi; Yingning Zhang; Nathan Jacobsen; Kirk Allin; Xiaofei Liu; Julia Hoffmann; Ognian Bohorov; Ola Blixt

doi:10.1016/j.carres.2006.03.043

Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens

Daniela Vasiliu, Nahid Razi, Yingning Zhang, Nathan Jacobsen, Kirk Allin, Xiaofei Liu, Julia Hoffmann, Ognian Bohorov, Ola Blixt

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

41 Citationer (Scopus)

Abstract

Udgivelsesdato: 2006-Jul-24

Originalsprog	Engelsk
Tidsskrift	Carbohydrate Research
Vol/bind	341
Udgave nummer	10
Sider (fra-til)	1447-57
Antal sider	10
ISSN	0008-6215
DOI	https://doi.org/10.1016/j.carres.2006.03.043
Status	Udgivet - 2006
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Antigens, Neoplasm; Blood Group Antigens; Fucosyltransferases; Polysaccharides; Sialyltransferases

Adgang til dokumentet

10.1016/j.carres.2006.03.043

Citationsformater

@article{986e132008bf11de8478000ea68e967b,

title = "Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens",

abstract = "Poly-N-acetyllactosamines (pLNs) are common terminal sugars of many N- and O-linked glycan structures present in glycoproteins and glycolipids. Utilizing various glycosyltransferases, we developed new and efficient chemoenzymatic methods for the synthesis of pLNs in gram-scale. Specifically, the use of sialyltransferases and fucosyltransferases enabled us to synthesize and purify 24 blood group and tumor-associated pLN derivatives with alpha-(2-->3)- and alpha-(2-->6)-linked sialic acid, as well as with alpha-(1-->2)- and alpha-(1-->3)-linked fucose. All synthesized derivatives were linked to a short 2-azidoethyl spacer for further modification.",

author = "Daniela Vasiliu and Nahid Razi and Yingning Zhang and Nathan Jacobsen and Kirk Allin and Xiaofei Liu and Julia Hoffmann and Ognian Bohorov and Ola Blixt",

note = "Keywords: Antigens, Neoplasm; Blood Group Antigens; Fucosyltransferases; Polysaccharides; Sialyltransferases",

year = "2006",

doi = "10.1016/j.carres.2006.03.043",

language = "English",

volume = "341",

pages = "1447--57",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Pergamon Press",

number = "10",

}

TY - JOUR

T1 - Large-scale chemoenzymatic synthesis of blood group and tumor-associated poly-N-acetyllactosamine antigens

AU - Vasiliu, Daniela

AU - Razi, Nahid

AU - Zhang, Yingning

AU - Jacobsen, Nathan

AU - Allin, Kirk

AU - Liu, Xiaofei

AU - Hoffmann, Julia

AU - Bohorov, Ognian

AU - Blixt, Ola

N1 - Keywords: Antigens, Neoplasm; Blood Group Antigens; Fucosyltransferases; Polysaccharides; Sialyltransferases

PY - 2006

Y1 - 2006

N2 - Poly-N-acetyllactosamines (pLNs) are common terminal sugars of many N- and O-linked glycan structures present in glycoproteins and glycolipids. Utilizing various glycosyltransferases, we developed new and efficient chemoenzymatic methods for the synthesis of pLNs in gram-scale. Specifically, the use of sialyltransferases and fucosyltransferases enabled us to synthesize and purify 24 blood group and tumor-associated pLN derivatives with alpha-(2-->3)- and alpha-(2-->6)-linked sialic acid, as well as with alpha-(1-->2)- and alpha-(1-->3)-linked fucose. All synthesized derivatives were linked to a short 2-azidoethyl spacer for further modification.

AB - Poly-N-acetyllactosamines (pLNs) are common terminal sugars of many N- and O-linked glycan structures present in glycoproteins and glycolipids. Utilizing various glycosyltransferases, we developed new and efficient chemoenzymatic methods for the synthesis of pLNs in gram-scale. Specifically, the use of sialyltransferases and fucosyltransferases enabled us to synthesize and purify 24 blood group and tumor-associated pLN derivatives with alpha-(2-->3)- and alpha-(2-->6)-linked sialic acid, as well as with alpha-(1-->2)- and alpha-(1-->3)-linked fucose. All synthesized derivatives were linked to a short 2-azidoethyl spacer for further modification.

U2 - 10.1016/j.carres.2006.03.043

DO - 10.1016/j.carres.2006.03.043

M3 - Journal article

C2 - 16650392

SN - 0008-6215

VL - 341

SP - 1447

EP - 1457

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 10

ER -