Laser capture microdissection-based in vivo genomic profiling of wound keratinocytes identifies similarities and differences to squamous cell carcinoma

Tanja Xenia Pedersen; Chidchanop Leethanakul; Vyomesh Patel; David Mitola; Leif Røge Lund; Keld Danø; Morten Johnsen; J Silvio Gutkind; Thomas Henrik Bugge

doi:10.1038/sj.onc.1206614

Laser capture microdissection-based in vivo genomic profiling of wound keratinocytes identifies similarities and differences to squamous cell carcinoma

Tanja Xenia Pedersen, Chidchanop Leethanakul, Vyomesh Patel, David Mitola, Leif Røge Lund, Keld Danø, Morten Johnsen, J Silvio Gutkind, Thomas Henrik Bugge

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

70 Citationer (Scopus)

Abstract

Udgivelsesdato: 2003-Jun-19

Originalsprog	Engelsk
Tidsskrift	Oncogene
Vol/bind	22
Udgave nummer	25
Sider (fra-til)	3964-76
Antal sider	12
ISSN	0950-9232
DOI	https://doi.org/10.1038/sj.onc.1206614
Status	Udgivet - 2003

Bibliografisk note

Keywords: Animals; Carcinoma, Squamous Cell; Cell Separation; Cell Transformation, Neoplastic; Epidermis; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; In Situ Hybridization; Keratinocytes; Lasers; Mice; Mice, Inbred C57BL; Oligonucleotide Array Sequence Analysis; Phenotype; RNA, Messenger; RNA, Neoplasm; Reproducibility of Results; Skin; Skin Neoplasms; Wound Healing

Adgang til dokumentet

10.1038/sj.onc.1206614

Citationsformater

@article{39c2ef300f4911df825d000ea68e967b,

title = "Laser capture microdissection-based in vivo genomic profiling of wound keratinocytes identifies similarities and differences to squamous cell carcinoma",

abstract = "Keratinocytes undergo a dramatic phenotypic conversion during reepithelialization of skin wounds to become hyperproliferative, migratory, and invasive. This transient healing response phenotypically resembles malignant transformation of keratinocytes during squamous cell carcinoma progression. Here we present the first analysis of global changes in keratinocyte gene expression during skin wound healing in vivo, and compare these changes to changes in gene expression during malignant conversion of keratinized epithelium. Laser capture microdissection was used to isolate RNA from wound keratinocytes from incisional mouse skin wounds and adjacent normal skin keratinocytes. Changes in gene expression were determined by comparative cDNA array analyses, and the approach was validated by in situ hybridization. The analyses identified 48 candidate genes not previously associated with wound reepithelialization. Furthermore, the analyses revealed that the phenotypic resemblance of wound keratinocytes to squamous cell carcinoma is mimicked at the level of gene expression, but notable differences between the two tissue-remodeling processes were also observed. The combination of laser capture microdissection and cDNA array analysis provides a powerful new tool to unravel the complex changes in gene expression that underlie physiological and pathological remodeling of keratinized epithelium.",

author = "Pedersen, {Tanja Xenia} and Chidchanop Leethanakul and Vyomesh Patel and David Mitola and Lund, {Leif R{\o}ge} and Keld Dan{\o} and Morten Johnsen and Gutkind, {J Silvio} and Bugge, {Thomas Henrik}",

note = "Keywords: Animals; Carcinoma, Squamous Cell; Cell Separation; Cell Transformation, Neoplastic; Epidermis; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; In Situ Hybridization; Keratinocytes; Lasers; Mice; Mice, Inbred C57BL; Oligonucleotide Array Sequence Analysis; Phenotype; RNA, Messenger; RNA, Neoplasm; Reproducibility of Results; Skin; Skin Neoplasms; Wound Healing",

year = "2003",

doi = "10.1038/sj.onc.1206614",

language = "English",

volume = "22",

pages = "3964--76",

journal = "Oncogene",

issn = "0950-9232",

publisher = "nature publishing group",

number = "25",

}

TY - JOUR

T1 - Laser capture microdissection-based in vivo genomic profiling of wound keratinocytes identifies similarities and differences to squamous cell carcinoma

AU - Pedersen, Tanja Xenia

AU - Leethanakul, Chidchanop

AU - Patel, Vyomesh

AU - Mitola, David

AU - Lund, Leif Røge

AU - Danø, Keld

AU - Johnsen, Morten

AU - Gutkind, J Silvio

AU - Bugge, Thomas Henrik

N1 - Keywords: Animals; Carcinoma, Squamous Cell; Cell Separation; Cell Transformation, Neoplastic; Epidermis; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; In Situ Hybridization; Keratinocytes; Lasers; Mice; Mice, Inbred C57BL; Oligonucleotide Array Sequence Analysis; Phenotype; RNA, Messenger; RNA, Neoplasm; Reproducibility of Results; Skin; Skin Neoplasms; Wound Healing

PY - 2003

Y1 - 2003

N2 - Keratinocytes undergo a dramatic phenotypic conversion during reepithelialization of skin wounds to become hyperproliferative, migratory, and invasive. This transient healing response phenotypically resembles malignant transformation of keratinocytes during squamous cell carcinoma progression. Here we present the first analysis of global changes in keratinocyte gene expression during skin wound healing in vivo, and compare these changes to changes in gene expression during malignant conversion of keratinized epithelium. Laser capture microdissection was used to isolate RNA from wound keratinocytes from incisional mouse skin wounds and adjacent normal skin keratinocytes. Changes in gene expression were determined by comparative cDNA array analyses, and the approach was validated by in situ hybridization. The analyses identified 48 candidate genes not previously associated with wound reepithelialization. Furthermore, the analyses revealed that the phenotypic resemblance of wound keratinocytes to squamous cell carcinoma is mimicked at the level of gene expression, but notable differences between the two tissue-remodeling processes were also observed. The combination of laser capture microdissection and cDNA array analysis provides a powerful new tool to unravel the complex changes in gene expression that underlie physiological and pathological remodeling of keratinized epithelium.

AB - Keratinocytes undergo a dramatic phenotypic conversion during reepithelialization of skin wounds to become hyperproliferative, migratory, and invasive. This transient healing response phenotypically resembles malignant transformation of keratinocytes during squamous cell carcinoma progression. Here we present the first analysis of global changes in keratinocyte gene expression during skin wound healing in vivo, and compare these changes to changes in gene expression during malignant conversion of keratinized epithelium. Laser capture microdissection was used to isolate RNA from wound keratinocytes from incisional mouse skin wounds and adjacent normal skin keratinocytes. Changes in gene expression were determined by comparative cDNA array analyses, and the approach was validated by in situ hybridization. The analyses identified 48 candidate genes not previously associated with wound reepithelialization. Furthermore, the analyses revealed that the phenotypic resemblance of wound keratinocytes to squamous cell carcinoma is mimicked at the level of gene expression, but notable differences between the two tissue-remodeling processes were also observed. The combination of laser capture microdissection and cDNA array analysis provides a powerful new tool to unravel the complex changes in gene expression that underlie physiological and pathological remodeling of keratinized epithelium.

U2 - 10.1038/sj.onc.1206614

DO - 10.1038/sj.onc.1206614

M3 - Journal article

C2 - 12813470

SN - 0950-9232

VL - 22

SP - 3964

EP - 3976

JO - Oncogene

JF - Oncogene

IS - 25

ER -