Ligand-induced TCR down-regulation is not dependent on constitutive TCR cycling

Jes Dietrich; Charlotte Menné; Jens Peter H Lauritsen; Marina von Essen; B. A. Rasmussen; Niels Ødum; Carsten Geisler

Ligand-induced TCR down-regulation is not dependent on constitutive TCR cycling

Jes Dietrich, Charlotte Menné, Jens Peter H Lauritsen, Marina von Essen, B. A. Rasmussen, Niels Ødum, Carsten Geisler

Cellebiologi og Fysiologi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

43 Citationer (Scopus)

Abstract

Udgivelsesdato: 2002-Jun-1

Originalsprog	Engelsk
Tidsskrift	Journal of Immunology
Vol/bind	168
Udgave nummer	11
Sider (fra-til)	5434-40
Antal sider	6
ISSN	0022-1767
Status	Udgivet - 2002

Bibliografisk note

Keywords: Amino Acid Motifs; Antigens, CD3; Down-Regulation; Humans; Jurkat Cells; Ligands; Protein Kinase C; Receptors, Antigen, T-Cell

Citationsformater

@article{787a9ba0b0a011ddb538000ea68e967b,

title = "Ligand-induced TCR down-regulation is not dependent on constitutive TCR cycling",

abstract = "TCR internalization takes place both in resting T cells as part of constitutive TCR cycling, after PKC activation, and during TCR triggering. It is still a matter of debate whether these pathways represent distinct pathways. Thus, some studies have indicated that ligand-induced TCR internalization is regulated by mechanisms distinct from those involved in constitutive internalization, whereas other studies have suggested that the ligand-induced TCR internalization pathway is identical with the constitutive pathway. To resolve this question, we first identified requirements for constitutive TCR cycling. We found that in contrast to PKC-induced TCR internalization where both CD3gamma-S(126) and the CD3gamma leucine-based internalization motif are required, constitutive TCR cycling required neither PKC nor CD3gamma-S(126) but only the CD3gamma leucine-based motif. Having identified these requirements, we next studied ligand-induced internalization in cells with abolished constitutive TCR cycling. We found that ligand-induced TCR internalization was not dependent on constitutive TCR internalization. Likewise, constitutive internalization and recycling of the TCR were independent of an intact ligand-induced internalization of the TCR. In conclusion, ligand-induced TCR internalization and constitutive cycling of the TCR represents two independent pathways regulated by different mechanisms.",

author = "Jes Dietrich and Charlotte Menn{\'e} and Lauritsen, {Jens Peter H} and {von Essen}, Marina and Rasmussen, {B. A.} and Niels {\O}dum and Carsten Geisler",

note = "Keywords: Amino Acid Motifs; Antigens, CD3; Down-Regulation; Humans; Jurkat Cells; Ligands; Protein Kinase C; Receptors, Antigen, T-Cell",

year = "2002",

language = "English",

volume = "168",

pages = "5434--40",

journal = "Journal of Immunology",

issn = "0022-1767",

publisher = "American Association of Immunologists",

number = "11",

}

TY - JOUR

T1 - Ligand-induced TCR down-regulation is not dependent on constitutive TCR cycling

AU - Dietrich, Jes

AU - Menné, Charlotte

AU - Lauritsen, Jens Peter H

AU - von Essen, Marina

AU - Rasmussen, B. A.

AU - Ødum, Niels

AU - Geisler, Carsten

N1 - Keywords: Amino Acid Motifs; Antigens, CD3; Down-Regulation; Humans; Jurkat Cells; Ligands; Protein Kinase C; Receptors, Antigen, T-Cell

PY - 2002

Y1 - 2002

N2 - TCR internalization takes place both in resting T cells as part of constitutive TCR cycling, after PKC activation, and during TCR triggering. It is still a matter of debate whether these pathways represent distinct pathways. Thus, some studies have indicated that ligand-induced TCR internalization is regulated by mechanisms distinct from those involved in constitutive internalization, whereas other studies have suggested that the ligand-induced TCR internalization pathway is identical with the constitutive pathway. To resolve this question, we first identified requirements for constitutive TCR cycling. We found that in contrast to PKC-induced TCR internalization where both CD3gamma-S(126) and the CD3gamma leucine-based internalization motif are required, constitutive TCR cycling required neither PKC nor CD3gamma-S(126) but only the CD3gamma leucine-based motif. Having identified these requirements, we next studied ligand-induced internalization in cells with abolished constitutive TCR cycling. We found that ligand-induced TCR internalization was not dependent on constitutive TCR internalization. Likewise, constitutive internalization and recycling of the TCR were independent of an intact ligand-induced internalization of the TCR. In conclusion, ligand-induced TCR internalization and constitutive cycling of the TCR represents two independent pathways regulated by different mechanisms.

AB - TCR internalization takes place both in resting T cells as part of constitutive TCR cycling, after PKC activation, and during TCR triggering. It is still a matter of debate whether these pathways represent distinct pathways. Thus, some studies have indicated that ligand-induced TCR internalization is regulated by mechanisms distinct from those involved in constitutive internalization, whereas other studies have suggested that the ligand-induced TCR internalization pathway is identical with the constitutive pathway. To resolve this question, we first identified requirements for constitutive TCR cycling. We found that in contrast to PKC-induced TCR internalization where both CD3gamma-S(126) and the CD3gamma leucine-based internalization motif are required, constitutive TCR cycling required neither PKC nor CD3gamma-S(126) but only the CD3gamma leucine-based motif. Having identified these requirements, we next studied ligand-induced internalization in cells with abolished constitutive TCR cycling. We found that ligand-induced TCR internalization was not dependent on constitutive TCR internalization. Likewise, constitutive internalization and recycling of the TCR were independent of an intact ligand-induced internalization of the TCR. In conclusion, ligand-induced TCR internalization and constitutive cycling of the TCR represents two independent pathways regulated by different mechanisms.

M3 - Journal article

C2 - 12023336

VL - 168

SP - 5434

EP - 5440

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 11

ER -