Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii

Marianne Kirstine Kjeldsen; Dorte Bek; Erik Michael Rasmussen; Anders Priemé; Vibeke Østergaard Thomsen

doi:10.1080/00365540903127425

Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii

Marianne Kirstine Kjeldsen, Dorte Bek, Erik Michael Rasmussen, Anders Priemé, Vibeke Østergaard Thomsen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

10 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-null

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Infectious Diseases
Vol/bind	41
Udgave nummer	9
Sider (fra-til)	635-41
Antal sider	6
ISSN	0036-5548
DOI	https://doi.org/10.1080/00365540903127425
Status	Udgivet - 2009

Adgang til dokumentet

10.1080/00365540903127425

Citationsformater

Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii. / Kjeldsen, Marianne Kirstine; Bek, Dorte; Rasmussen, Erik Michael et al.
I: Scandinavian Journal of Infectious Diseases, Bind 41, Nr. 9, 2009, s. 635-41.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{5dc424a030df11df8ed1000ea68e967b,

title = "Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii",

abstract = "A line probe assay (GenoType MTBC) was evaluated for species differentiation within the Mycobacterium tuberculosis complex (MTBC). We included 387 MTBC isolates, 43 IS6110 low-copy MTBC isolates, 28 clinical specimens with varying microscopy grade, and 30 isolates of non-tuberculous mycobacteria. The assay was 100% specific and identified all 387 isolates and 98% of all IS6110 low-copy strains in concordance with the gold standard. The 2% discrepancy was caused by 1 isolate showing a faint restriction fragment length polymorphism (RFLP) pattern. The assay could provide specifies identification in 13 of 19 (68%) microscopy-positive specimens and 0 of 9 microscopy-negative specimens. To our surprise, the probe for M. africanum subtype I reacted with M. pinnipedii. This cross-reaction has not previously been reported. The assay was rapid, easy to perform and directly applicable in highly smear-positive specimens. We predict that the assay will enable enhanced surveillance of species-specific treatment outcome, which may change treatment regimens.",

author = "Kjeldsen, {Marianne Kirstine} and Dorte Bek and Rasmussen, {Erik Michael} and Anders Priem{\'e} and Thomsen, {Vibeke {\O}stergaard}",

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T1 - Line probe assay for differentiation within Mycobacterium tuberculosis complex. Evaluation on clinical specimens and isolates including Mycobacterium pinnipedii

AU - Kjeldsen, Marianne Kirstine

AU - Bek, Dorte

AU - Rasmussen, Erik Michael

AU - Priemé, Anders

AU - Thomsen, Vibeke Østergaard

PY - 2009

Y1 - 2009

N2 - A line probe assay (GenoType MTBC) was evaluated for species differentiation within the Mycobacterium tuberculosis complex (MTBC). We included 387 MTBC isolates, 43 IS6110 low-copy MTBC isolates, 28 clinical specimens with varying microscopy grade, and 30 isolates of non-tuberculous mycobacteria. The assay was 100% specific and identified all 387 isolates and 98% of all IS6110 low-copy strains in concordance with the gold standard. The 2% discrepancy was caused by 1 isolate showing a faint restriction fragment length polymorphism (RFLP) pattern. The assay could provide specifies identification in 13 of 19 (68%) microscopy-positive specimens and 0 of 9 microscopy-negative specimens. To our surprise, the probe for M. africanum subtype I reacted with M. pinnipedii. This cross-reaction has not previously been reported. The assay was rapid, easy to perform and directly applicable in highly smear-positive specimens. We predict that the assay will enable enhanced surveillance of species-specific treatment outcome, which may change treatment regimens.

AB - A line probe assay (GenoType MTBC) was evaluated for species differentiation within the Mycobacterium tuberculosis complex (MTBC). We included 387 MTBC isolates, 43 IS6110 low-copy MTBC isolates, 28 clinical specimens with varying microscopy grade, and 30 isolates of non-tuberculous mycobacteria. The assay was 100% specific and identified all 387 isolates and 98% of all IS6110 low-copy strains in concordance with the gold standard. The 2% discrepancy was caused by 1 isolate showing a faint restriction fragment length polymorphism (RFLP) pattern. The assay could provide specifies identification in 13 of 19 (68%) microscopy-positive specimens and 0 of 9 microscopy-negative specimens. To our surprise, the probe for M. africanum subtype I reacted with M. pinnipedii. This cross-reaction has not previously been reported. The assay was rapid, easy to perform and directly applicable in highly smear-positive specimens. We predict that the assay will enable enhanced surveillance of species-specific treatment outcome, which may change treatment regimens.

U2 - 10.1080/00365540903127425

DO - 10.1080/00365540903127425

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C2 - 20001279

SN - 0036-5548

VL - 41

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JO - Scandinavian Journal of Infectious Diseases

JF - Scandinavian Journal of Infectious Diseases

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