Lipid raft localization of GABA A receptor and Na+, K+-ATPase in discrete microdomain clusters in rat cerebellar granule cells

Stine-Mathilde Dalskov; Lissi Immerdal; Lise-Lotte W Niels-Christiansen; Gert Helge Hansen; Arne Schousboe; Erik Michael Danielsen

doi:10.1016/j.neuint.2004.11.010

Lipid raft localization of GABA A receptor and Na+, K+-ATPase in discrete microdomain clusters in rat cerebellar granule cells

Stine-Mathilde Dalskov, Lissi Immerdal, Lise-Lotte W Niels-Christiansen, Gert Helge Hansen, Arne Schousboe, Erik Michael Danielsen

Institut for Cellulær og Molekylær Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

59 Citationer (Scopus)

Abstract

Udgivelsesdato: 2005-May

Originalsprog	Engelsk
Tidsskrift	Neurochemistry International
Vol/bind	46
Udgave nummer	6
Sider (fra-til)	489-99
Antal sider	10
ISSN	0197-0186
DOI	https://doi.org/10.1016/j.neuint.2004.11.010
Status	Udgivet - 2005

Bibliografisk note

Keywords: Animals; Animals, Newborn; Ankyrins; Carrier Proteins; Cells, Cultured; Cerebellar Cortex; Cholesterol; Detergents; G(M1) Ganglioside; Immunohistochemistry; Membrane Microdomains; Membrane Proteins; Microscopy, Electron, Transmission; Neural Inhibition; Neurons; Rats; Receptor Aggregation; Receptors, GABA-A; Sodium-Potassium-Exchanging ATPase; Solubility; Subcellular Fractions; Synaptic Transmission

Adgang til dokumentet

10.1016/j.neuint.2004.11.010

Citationsformater

@article{c28e9b00e3be11ddbf70000ea68e967b,

title = "Lipid raft localization of GABA A receptor and Na+, K+-ATPase in discrete microdomain clusters in rat cerebellar granule cells",

abstract = "The microdomain localization of the GABA(A) receptor in rat cerebellar granule cells was studied by subcellular fractionation and fluorescence- and immunogold electron microscopy. The receptor resided in lipid rafts, prepared at 37 degrees C by extraction with the nonionic detergent Brij 98, but the raft fraction, defined by the marker ganglioside GM(1) in the floating fractions following density gradient centrifugation, was heterogeneous in density and protein composition. Thus, another major raft-associated membrane protein, the Na(+), K(+)-ATPase, was found in discrete rafts of lower density, reflecting clustering of the two proteins in separate membrane microdomains. Both proteins were observed in patchy {"}hot spots{"} at the cell surface as well as in isolated lipid rafts. Their insolubility in Brij 98 was only marginally affected by methyl-beta-cyclodextrin. In contrast, both the GABA(A) receptor and Na(+), K(+)-ATPase were largely soluble in ice cold Triton X-100. This indicates that Brij 98 extraction defines an unusual type of cholesterol-independent lipid rafts that harbour membrane proteins also associated with underlying scaffolding/cytoskeletal proteins such as gephyrin (GABA(A) receptor) and ankyrin G (Na(+), K(+)-ATPase). By providing an ordered membrane microenvironment, lipid rafts may contribute to the clustering of the GABA(A) receptor and the Na(+), K(+)-ATPase at distinct functional locations on the cell surface.",

author = "Stine-Mathilde Dalskov and Lissi Immerdal and Niels-Christiansen, {Lise-Lotte W} and Hansen, {Gert Helge} and Arne Schousboe and Danielsen, {Erik Michael}",

note = "Keywords: Animals; Animals, Newborn; Ankyrins; Carrier Proteins; Cells, Cultured; Cerebellar Cortex; Cholesterol; Detergents; G(M1) Ganglioside; Immunohistochemistry; Membrane Microdomains; Membrane Proteins; Microscopy, Electron, Transmission; Neural Inhibition; Neurons; Rats; Receptor Aggregation; Receptors, GABA-A; Sodium-Potassium-Exchanging ATPase; Solubility; Subcellular Fractions; Synaptic Transmission",

year = "2005",

doi = "10.1016/j.neuint.2004.11.010",

language = "English",

volume = "46",

pages = "489--99",

journal = "Neurochemistry International",

issn = "0197-0186",

publisher = "Elsevier",

number = "6",

}

TY - JOUR

T1 - Lipid raft localization of GABA A receptor and Na+, K+-ATPase in discrete microdomain clusters in rat cerebellar granule cells

AU - Dalskov, Stine-Mathilde

AU - Immerdal, Lissi

AU - Niels-Christiansen, Lise-Lotte W

AU - Hansen, Gert Helge

AU - Schousboe, Arne

AU - Danielsen, Erik Michael

N1 - Keywords: Animals; Animals, Newborn; Ankyrins; Carrier Proteins; Cells, Cultured; Cerebellar Cortex; Cholesterol; Detergents; G(M1) Ganglioside; Immunohistochemistry; Membrane Microdomains; Membrane Proteins; Microscopy, Electron, Transmission; Neural Inhibition; Neurons; Rats; Receptor Aggregation; Receptors, GABA-A; Sodium-Potassium-Exchanging ATPase; Solubility; Subcellular Fractions; Synaptic Transmission

PY - 2005

Y1 - 2005

N2 - The microdomain localization of the GABA(A) receptor in rat cerebellar granule cells was studied by subcellular fractionation and fluorescence- and immunogold electron microscopy. The receptor resided in lipid rafts, prepared at 37 degrees C by extraction with the nonionic detergent Brij 98, but the raft fraction, defined by the marker ganglioside GM(1) in the floating fractions following density gradient centrifugation, was heterogeneous in density and protein composition. Thus, another major raft-associated membrane protein, the Na(+), K(+)-ATPase, was found in discrete rafts of lower density, reflecting clustering of the two proteins in separate membrane microdomains. Both proteins were observed in patchy "hot spots" at the cell surface as well as in isolated lipid rafts. Their insolubility in Brij 98 was only marginally affected by methyl-beta-cyclodextrin. In contrast, both the GABA(A) receptor and Na(+), K(+)-ATPase were largely soluble in ice cold Triton X-100. This indicates that Brij 98 extraction defines an unusual type of cholesterol-independent lipid rafts that harbour membrane proteins also associated with underlying scaffolding/cytoskeletal proteins such as gephyrin (GABA(A) receptor) and ankyrin G (Na(+), K(+)-ATPase). By providing an ordered membrane microenvironment, lipid rafts may contribute to the clustering of the GABA(A) receptor and the Na(+), K(+)-ATPase at distinct functional locations on the cell surface.

AB - The microdomain localization of the GABA(A) receptor in rat cerebellar granule cells was studied by subcellular fractionation and fluorescence- and immunogold electron microscopy. The receptor resided in lipid rafts, prepared at 37 degrees C by extraction with the nonionic detergent Brij 98, but the raft fraction, defined by the marker ganglioside GM(1) in the floating fractions following density gradient centrifugation, was heterogeneous in density and protein composition. Thus, another major raft-associated membrane protein, the Na(+), K(+)-ATPase, was found in discrete rafts of lower density, reflecting clustering of the two proteins in separate membrane microdomains. Both proteins were observed in patchy "hot spots" at the cell surface as well as in isolated lipid rafts. Their insolubility in Brij 98 was only marginally affected by methyl-beta-cyclodextrin. In contrast, both the GABA(A) receptor and Na(+), K(+)-ATPase were largely soluble in ice cold Triton X-100. This indicates that Brij 98 extraction defines an unusual type of cholesterol-independent lipid rafts that harbour membrane proteins also associated with underlying scaffolding/cytoskeletal proteins such as gephyrin (GABA(A) receptor) and ankyrin G (Na(+), K(+)-ATPase). By providing an ordered membrane microenvironment, lipid rafts may contribute to the clustering of the GABA(A) receptor and the Na(+), K(+)-ATPase at distinct functional locations on the cell surface.

U2 - 10.1016/j.neuint.2004.11.010

DO - 10.1016/j.neuint.2004.11.010

M3 - Journal article

C2 - 15769551

SN - 0197-0186

VL - 46

SP - 489

EP - 499

JO - Neurochemistry International

JF - Neurochemistry International

IS - 6

ER -