Liquid Biopsies in Follicular Thyroid Carcinomas - A Brief Report

Marie-Louise Uhre Hansen; Simone Kloch Bendtsen; Kathrine Kronberg Jakobsen; Ane Yde Schmidt; Christoffer Holst Hahn; Christian von Buchwald; Christian Grønhøj

doi:10.3390/diagnostics14141577

Liquid Biopsies in Follicular Thyroid Carcinomas - A Brief Report

Marie-Louise Uhre Hansen^*, Simone Kloch Bendtsen, Kathrine Kronberg Jakobsen, Ane Yde Schmidt, Christoffer Holst Hahn, Christian von Buchwald, Christian Grønhøj

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

1 Citationer (Scopus)

17 Downloads (Pure)

Abstract

Thyroid cancer (TC) represents a significant health burden globally, with follicular thyroid cancer (FTC) posing diagnostic challenges despite advancements. This pilot study aimed to evaluate the utility of a liquid biopsy with cell-free DNA (cfDNA) in patients with FTC. Blood samples were collected from 13 patients diagnosed with FTC, DNA extraction was performed, and cfDNA was analyzed using the Illumina’s TruSight Oncology 500 High-Throughput panel. The results revealed low tumor mutational burden and minimal pathogenic variants in cfDNA, indicating challenges such as low DNA yield and poor material quality despite adequate coverage. Our findings indicate that cfDNA as an add-on diagnostic tool in patients with FTC might not be a useful supplement.

Originalsprog	Engelsk
Artikelnummer	1577
Tidsskrift	Diagnostics
Vol/bind	14
Udgave nummer	14
Antal sider	10
ISSN	2075-4418
DOI	https://doi.org/10.3390/diagnostics14141577
Status	Udgivet - 2024

Bibliografisk note

Funding Information:
This research was funded by Ear Doctor Hans Skouby\u2019s og Wife Emma Skouby\u2019s Fundation. Carl og Ellen Hertz\u2019 Scholasrship. Manufacturer Einar Willumsens Memorial Grant.

Publisher Copyright:
© 2024 by the authors.

Adgang til dokumentet

10.3390/diagnostics14141577Licens: CC BY

Full textForlagets udgivne version, 2,81 MBLicens: CC BY

Citationsformater

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abstract = "Thyroid cancer (TC) represents a significant health burden globally, with follicular thyroid cancer (FTC) posing diagnostic challenges despite advancements. This pilot study aimed to evaluate the utility of a liquid biopsy with cell-free DNA (cfDNA) in patients with FTC. Blood samples were collected from 13 patients diagnosed with FTC, DNA extraction was performed, and cfDNA was analyzed using the Illumina{\textquoteright}s TruSight Oncology 500 High-Throughput panel. The results revealed low tumor mutational burden and minimal pathogenic variants in cfDNA, indicating challenges such as low DNA yield and poor material quality despite adequate coverage. Our findings indicate that cfDNA as an add-on diagnostic tool in patients with FTC might not be a useful supplement.",

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AU - Hansen, Marie-Louise Uhre

AU - Bendtsen, Simone Kloch

AU - Jakobsen, Kathrine Kronberg

AU - Schmidt, Ane Yde

AU - Hahn, Christoffer Holst

AU - von Buchwald, Christian

AU - Grønhøj, Christian

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AB - Thyroid cancer (TC) represents a significant health burden globally, with follicular thyroid cancer (FTC) posing diagnostic challenges despite advancements. This pilot study aimed to evaluate the utility of a liquid biopsy with cell-free DNA (cfDNA) in patients with FTC. Blood samples were collected from 13 patients diagnosed with FTC, DNA extraction was performed, and cfDNA was analyzed using the Illumina’s TruSight Oncology 500 High-Throughput panel. The results revealed low tumor mutational burden and minimal pathogenic variants in cfDNA, indicating challenges such as low DNA yield and poor material quality despite adequate coverage. Our findings indicate that cfDNA as an add-on diagnostic tool in patients with FTC might not be a useful supplement.

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