Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Cell Biology International |
| Vol/bind | 29 |
| Udgave nummer | 5 |
| Sider (fra-til) | 340-6 |
| Antal sider | 6 |
| ISSN | 1065-6995 |
| DOI | |
| Status | Udgivet - 2005 |
Bibliografisk note
Keywords: Animals; Cilia; Female; Humans; Mice; Mice, Inbred C57BL; Ovary; Oviducts; Receptor, TIE-1; Receptor, TIE-2Adgang til dokumentet
Citationsformater
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS
I: Cell Biology International, Bind 29, Nr. 5, 2005, s. 340-6.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
}
TY - JOUR
T1 - Localization of the angiopoietin receptors Tie-1 and Tie-2 on the primary cilia in the female reproductive organs
AU - Teilmann, Stefan C
AU - Christensen, Søren T
N1 - Keywords: Animals; Cilia; Female; Humans; Mice; Mice, Inbred C57BL; Ovary; Oviducts; Receptor, TIE-1; Receptor, TIE-2
PY - 2005
Y1 - 2005
N2 - Blood vessel homeostasis and endothelial cell survival depend on proper signalling through angiopoietin receptors such as the receptor tyrosine kinases Tie-1 and Tie-2. We have studied the presence and subcellular localization of these receptors in murine female reproductive organs using confocal microscopy analysis of antibody stained tissue sections of ovary and oviduct. We show that Tie-2 principally localizes to primary cilia of the surface epithelium of the ovary, bursa and extra-ovarian rete ducts as well as to plasma membranes of ovarian theca and endothelial cells. Primary cilia of follicular granulosa cells were negative. Further, Tie-1 and Tie-2 localized to motile cilia of the oviduct. Western blotting detection and immunolocalization of anti-Tie-2 in ovary and oviduct were abolished by administration of an anti-Tie-2 blocking peptide, confirming antibody specificity. In a series of immunohistochemical analysis on human ovarian tissues we also observed a unique localization of Tie-2 to the primary cilia of ovarian surface epithelium. These observations are the first to show ciliary localization of angiopoietin receptors. Our results support the hypothesis that cilia of the female reproductive organs play a novel and important sensory role in relaying physiochemical changes from the extracellular environment to epithelial cells of the oviduct, the ovary and extra-ovarian tissues.
AB - Blood vessel homeostasis and endothelial cell survival depend on proper signalling through angiopoietin receptors such as the receptor tyrosine kinases Tie-1 and Tie-2. We have studied the presence and subcellular localization of these receptors in murine female reproductive organs using confocal microscopy analysis of antibody stained tissue sections of ovary and oviduct. We show that Tie-2 principally localizes to primary cilia of the surface epithelium of the ovary, bursa and extra-ovarian rete ducts as well as to plasma membranes of ovarian theca and endothelial cells. Primary cilia of follicular granulosa cells were negative. Further, Tie-1 and Tie-2 localized to motile cilia of the oviduct. Western blotting detection and immunolocalization of anti-Tie-2 in ovary and oviduct were abolished by administration of an anti-Tie-2 blocking peptide, confirming antibody specificity. In a series of immunohistochemical analysis on human ovarian tissues we also observed a unique localization of Tie-2 to the primary cilia of ovarian surface epithelium. These observations are the first to show ciliary localization of angiopoietin receptors. Our results support the hypothesis that cilia of the female reproductive organs play a novel and important sensory role in relaying physiochemical changes from the extracellular environment to epithelial cells of the oviduct, the ovary and extra-ovarian tissues.
U2 - 10.1016/j.cellbi.2005.03.006
DO - 10.1016/j.cellbi.2005.03.006
M3 - Journal article
C2 - 15893943
SN - 1065-6995
VL - 29
SP - 340
EP - 346
JO - Cell Biology International
JF - Cell Biology International
IS - 5
ER -