Long-term stability and circadian variation in circulating levels of surfactant protein D

Silje Vermedal Hoegh; Grith Lykke Sorensen; Ida Tornoe; Tine Lottenburger; Henriette Ytting; Hans Jørgen Nielsen; Peter Junker; Uffe Holmskov; Silje Vermedal Hoegh; Grith Lykke Sorensen; Ida Tornoe; Tine Lottenburger; Henriette Ytting; Hans Jørgen Nielsen; Peter Junker; Uffe Holmskov

doi:10.1016/j.imbio.2009.05.001

Long-term stability and circadian variation in circulating levels of surfactant protein D

Silje Vermedal Hoegh, Grith Lykke Sorensen, Ida Tornoe, Tine Lottenburger, Henriette Ytting, Hans Jørgen Nielsen, Peter Junker, Uffe Holmskov, Silje Vermedal Hoegh, Grith Lykke Sorensen, Ida Tornoe, Tine Lottenburger, Henriette Ytting, Hans Jørgen Nielsen, Peter Junker, Uffe Holmskov

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

28 Citationer (Scopus)

Abstract

Udgivelsesdato: 2010-Apr

Originalsprog	Engelsk
Tidsskrift	Immunobiology
Vol/bind	215
Udgave nummer	4
Sider (fra-til)	314-20
Antal sider	6
ISSN	0171-2985
DOI	https://doi.org/10.1016/j.imbio.2009.05.001
Status	Udgivet - 2010

Bibliografisk note

Adgang til dokumentet

10.1016/j.imbio.2009.05.001

Citationsformater

Hoegh, S. V., Sorensen, G. L., Tornoe, I., Lottenburger, T., Ytting, H., Nielsen, H. J., Junker, P., Holmskov, U., Hoegh, S. V., Sorensen, G. L., Tornoe, I., Lottenburger, T., Ytting, H., Nielsen, H. J., Junker, P., & Holmskov, U. (2010). Long-term stability and circadian variation in circulating levels of surfactant protein D. Immunobiology, 215(4), 314-20. https://doi.org/10.1016/j.imbio.2009.05.001

Hoegh, SV, Sorensen, GL, Tornoe, I, Lottenburger, T, Ytting, H, Nielsen, HJ, Junker, P, Holmskov, U, Hoegh, SV, Sorensen, GL, Tornoe, I, Lottenburger, T, Ytting, H, Nielsen, HJ, Junker, P & Holmskov, U 2010, 'Long-term stability and circadian variation in circulating levels of surfactant protein D', Immunobiology, bind 215, nr. 4, s. 314-20. https://doi.org/10.1016/j.imbio.2009.05.001

@article{f8fd2040aac211df928f000ea68e967b,

title = "Long-term stability and circadian variation in circulating levels of surfactant protein D",

abstract = "Surfactant protein D (SP-D) is an oligomeric calcium-dependent lectin with important roles in innate host defence against infectious microorganisms. Several studies have shown that patients with inflammatory lung disease have elevated levels of circulating SP-D, and serum SP-D has been suggested to be used as a biomarker for disease e.g. in COPD. We aimed to investigate the variation of circulating SP-D in healthy individuals in and between days for 6 months. In addition, we studied the SP-D response to a standardized physical exercise programme. SP-D was measured in serum using a 5-layered ELISA technique. We found that circulating SP-D remained constant over a 6-month period. However, during the course of one day SP-D varied significantly. Median SP-D peaked at 10 a.m. at 1009 ng/ml (95% CI: 803-1497), subsequently decreasing to nadir at 10 p.m. at 867 ng/ml (95% CI: 650-1148)(P<0.00005). Median pre-exercise level of SP-D was 746 ng/ml (95% CI: 384-2035), and immediately after cessation of physical activity the median SP-D level was 767 ng/ml (95% CI: 367-1885) (P=0.248). Our findings underscore the importance of standardized blood sampling conditions in future studies on the potential role of SP-D as a biomarker. Importantly, stable measures of systemic SP-D over a prolonged period support that SP-D is suitable for biomarker studies.",

author = "Hoegh, {Silje Vermedal} and Sorensen, {Grith Lykke} and Ida Tornoe and Tine Lottenburger and Henriette Ytting and Nielsen, {Hans J{\o}rgen} and Peter Junker and Uffe Holmskov and Hoegh, {Silje Vermedal} and Sorensen, {Grith Lykke} and Ida Tornoe and Tine Lottenburger and Henriette Ytting and Nielsen, {Hans J{\o}rgen} and Peter Junker and Uffe Holmskov",

note = "Keywords: Adult; Aged; Circadian Rhythm; Exercise; Female; Humans; Male; Middle Aged; Protein Stability; Pulmonary Surfactant-Associated Protein D",

year = "2010",

doi = "10.1016/j.imbio.2009.05.001",

language = "English",

volume = "215",

pages = "314--20",

journal = "Immunobiology",

issn = "0171-2985",

publisher = "Urban und Fischer Verlag",

number = "4",

}

TY - JOUR

T1 - Long-term stability and circadian variation in circulating levels of surfactant protein D

AU - Hoegh, Silje Vermedal

AU - Sorensen, Grith Lykke

AU - Tornoe, Ida

AU - Lottenburger, Tine

AU - Ytting, Henriette

AU - Nielsen, Hans Jørgen

AU - Junker, Peter

AU - Holmskov, Uffe

AU - Hoegh, Silje Vermedal

AU - Sorensen, Grith Lykke

AU - Tornoe, Ida

AU - Lottenburger, Tine

AU - Ytting, Henriette

AU - Nielsen, Hans Jørgen

AU - Junker, Peter

AU - Holmskov, Uffe

N1 - Keywords: Adult; Aged; Circadian Rhythm; Exercise; Female; Humans; Male; Middle Aged; Protein Stability; Pulmonary Surfactant-Associated Protein D

PY - 2010

Y1 - 2010

N2 - Surfactant protein D (SP-D) is an oligomeric calcium-dependent lectin with important roles in innate host defence against infectious microorganisms. Several studies have shown that patients with inflammatory lung disease have elevated levels of circulating SP-D, and serum SP-D has been suggested to be used as a biomarker for disease e.g. in COPD. We aimed to investigate the variation of circulating SP-D in healthy individuals in and between days for 6 months. In addition, we studied the SP-D response to a standardized physical exercise programme. SP-D was measured in serum using a 5-layered ELISA technique. We found that circulating SP-D remained constant over a 6-month period. However, during the course of one day SP-D varied significantly. Median SP-D peaked at 10 a.m. at 1009 ng/ml (95% CI: 803-1497), subsequently decreasing to nadir at 10 p.m. at 867 ng/ml (95% CI: 650-1148)(P<0.00005). Median pre-exercise level of SP-D was 746 ng/ml (95% CI: 384-2035), and immediately after cessation of physical activity the median SP-D level was 767 ng/ml (95% CI: 367-1885) (P=0.248). Our findings underscore the importance of standardized blood sampling conditions in future studies on the potential role of SP-D as a biomarker. Importantly, stable measures of systemic SP-D over a prolonged period support that SP-D is suitable for biomarker studies.

AB - Surfactant protein D (SP-D) is an oligomeric calcium-dependent lectin with important roles in innate host defence against infectious microorganisms. Several studies have shown that patients with inflammatory lung disease have elevated levels of circulating SP-D, and serum SP-D has been suggested to be used as a biomarker for disease e.g. in COPD. We aimed to investigate the variation of circulating SP-D in healthy individuals in and between days for 6 months. In addition, we studied the SP-D response to a standardized physical exercise programme. SP-D was measured in serum using a 5-layered ELISA technique. We found that circulating SP-D remained constant over a 6-month period. However, during the course of one day SP-D varied significantly. Median SP-D peaked at 10 a.m. at 1009 ng/ml (95% CI: 803-1497), subsequently decreasing to nadir at 10 p.m. at 867 ng/ml (95% CI: 650-1148)(P<0.00005). Median pre-exercise level of SP-D was 746 ng/ml (95% CI: 384-2035), and immediately after cessation of physical activity the median SP-D level was 767 ng/ml (95% CI: 367-1885) (P=0.248). Our findings underscore the importance of standardized blood sampling conditions in future studies on the potential role of SP-D as a biomarker. Importantly, stable measures of systemic SP-D over a prolonged period support that SP-D is suitable for biomarker studies.

U2 - 10.1016/j.imbio.2009.05.001

DO - 10.1016/j.imbio.2009.05.001

M3 - Journal article

C2 - 19540617

SN - 0171-2985

VL - 215

SP - 314

EP - 320

JO - Immunobiology

JF - Immunobiology

IS - 4

ER -