Lysyl oxidase-like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors

Sheri F T Fong; Erin Dietzsch; Keith S K Fong; Peter Hollosi; Lloyd Asuncion; Qingping He; M Iqbal Parker; Katalin Csiszar

doi:10.1002/gcc.20444

Lysyl oxidase-like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors

Sheri F T Fong, Erin Dietzsch, Keith S K Fong, Peter Hollosi, Lloyd Asuncion, Qingping He, M Iqbal Parker, Katalin Csiszar

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

103 Citationer (Scopus)

Abstract

Udgivelsesdato: 2007-Jul

Originalsprog	Engelsk
Tidsskrift	Genes, Chromosomes & Cancer
Vol/bind	46
Udgave nummer	7
Sider (fra-til)	644-55
Antal sider	11
ISSN	1045-2257
DOI	https://doi.org/10.1002/gcc.20444
Status	Udgivet - 2007
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Adult; Aged; Aged, 80 and over; Amino Acid Oxidoreductases; Azacitidine; Blotting, Western; Cell Differentiation; Cell Line; Colonic Neoplasms; CpG Islands; DNA Primers; Esophageal Neoplasms; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Loss of Heterozygosity; Male; Middle Aged; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction

Adgang til dokumentet

10.1002/gcc.20444

Citationsformater

@article{cfb75a5032ba11df8ed1000ea68e967b,

title = "Lysyl oxidase-like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors",

abstract = "Lysyl oxidase-like 2 (LOXL2) belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. We previously demonstrated the association between increased LOXL2 expression and invasive/metastatic behavior in human breast cancer cells and mouse squamous and spindle cell carcinomas, interaction between LOXL2 and SNAIL in epithelial-mesenchymal transition, and localization of the LOXL2 gene to 8p21.2-21.3, within a minimally deleted region in several cancers, including colon and esophagus. In the present study, we analyzed LOXL2 expression in colon and esophageal tumors, and explored methylation as a regulator of LOXL2 expression. Immunohistochemistry using normal tissues demonstrated intracellular localization of LOXL2 in colonic enteroendocrine cells and esophageal squamous cells at the luminal surface, but not in mitotically active cells. Tissue array analysis of 52 colon adenocarcinomas and 50 esophageal squamous cell carcinomas revealed presence of LOXL2 expression in 83 and 92% of the samples, respectively, and a significant association between increased number of LOXL2-expressing cells and less-differentiated colon carcinomas. We determined that the methylation status of the 1150 bp 5' CpG island may contribute to the regulation of the gene. Loss of heterozygosity studies, using a microsatellite within intron 4 of the LOXL2 gene, revealed that loss of LOXL2 was unlikely to play a major role in either colon or esophageal tumors. These results suggest that increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression.",

author = "Fong, {Sheri F T} and Erin Dietzsch and Fong, {Keith S K} and Peter Hollosi and Lloyd Asuncion and Qingping He and Parker, {M Iqbal} and Katalin Csiszar",

note = "Keywords: Adult; Aged; Aged, 80 and over; Amino Acid Oxidoreductases; Azacitidine; Blotting, Western; Cell Differentiation; Cell Line; Colonic Neoplasms; CpG Islands; DNA Primers; Esophageal Neoplasms; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Loss of Heterozygosity; Male; Middle Aged; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction",

year = "2007",

doi = "10.1002/gcc.20444",

language = "English",

volume = "46",

pages = "644--55",

journal = "Genes, Chromosomes & Cancer",

issn = "1045-2257",

publisher = "JohnWiley & Sons, Inc.",

number = "7",

}

TY - JOUR

T1 - Lysyl oxidase-like 2 expression is increased in colon and esophageal tumors and associated with less differentiated colon tumors

AU - Fong, Sheri F T

AU - Dietzsch, Erin

AU - Fong, Keith S K

AU - Hollosi, Peter

AU - Asuncion, Lloyd

AU - He, Qingping

AU - Parker, M Iqbal

AU - Csiszar, Katalin

N1 - Keywords: Adult; Aged; Aged, 80 and over; Amino Acid Oxidoreductases; Azacitidine; Blotting, Western; Cell Differentiation; Cell Line; Colonic Neoplasms; CpG Islands; DNA Primers; Esophageal Neoplasms; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Loss of Heterozygosity; Male; Middle Aged; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction

PY - 2007

Y1 - 2007

N2 - Lysyl oxidase-like 2 (LOXL2) belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. We previously demonstrated the association between increased LOXL2 expression and invasive/metastatic behavior in human breast cancer cells and mouse squamous and spindle cell carcinomas, interaction between LOXL2 and SNAIL in epithelial-mesenchymal transition, and localization of the LOXL2 gene to 8p21.2-21.3, within a minimally deleted region in several cancers, including colon and esophagus. In the present study, we analyzed LOXL2 expression in colon and esophageal tumors, and explored methylation as a regulator of LOXL2 expression. Immunohistochemistry using normal tissues demonstrated intracellular localization of LOXL2 in colonic enteroendocrine cells and esophageal squamous cells at the luminal surface, but not in mitotically active cells. Tissue array analysis of 52 colon adenocarcinomas and 50 esophageal squamous cell carcinomas revealed presence of LOXL2 expression in 83 and 92% of the samples, respectively, and a significant association between increased number of LOXL2-expressing cells and less-differentiated colon carcinomas. We determined that the methylation status of the 1150 bp 5' CpG island may contribute to the regulation of the gene. Loss of heterozygosity studies, using a microsatellite within intron 4 of the LOXL2 gene, revealed that loss of LOXL2 was unlikely to play a major role in either colon or esophageal tumors. These results suggest that increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression.

AB - Lysyl oxidase-like 2 (LOXL2) belongs to an amine oxidase family whose members have been implicated in crosslink formation in stromal collagens and elastin, cell motility, and tumor development and progression. We previously demonstrated the association between increased LOXL2 expression and invasive/metastatic behavior in human breast cancer cells and mouse squamous and spindle cell carcinomas, interaction between LOXL2 and SNAIL in epithelial-mesenchymal transition, and localization of the LOXL2 gene to 8p21.2-21.3, within a minimally deleted region in several cancers, including colon and esophagus. In the present study, we analyzed LOXL2 expression in colon and esophageal tumors, and explored methylation as a regulator of LOXL2 expression. Immunohistochemistry using normal tissues demonstrated intracellular localization of LOXL2 in colonic enteroendocrine cells and esophageal squamous cells at the luminal surface, but not in mitotically active cells. Tissue array analysis of 52 colon adenocarcinomas and 50 esophageal squamous cell carcinomas revealed presence of LOXL2 expression in 83 and 92% of the samples, respectively, and a significant association between increased number of LOXL2-expressing cells and less-differentiated colon carcinomas. We determined that the methylation status of the 1150 bp 5' CpG island may contribute to the regulation of the gene. Loss of heterozygosity studies, using a microsatellite within intron 4 of the LOXL2 gene, revealed that loss of LOXL2 was unlikely to play a major role in either colon or esophageal tumors. These results suggest that increased LOXL2 expression in colon and esophageal cancer may contribute to tumor progression.

U2 - 10.1002/gcc.20444

DO - 10.1002/gcc.20444

M3 - Journal article

C2 - 17394133

SN - 1045-2257

VL - 46

SP - 644

EP - 655

JO - Genes, Chromosomes & Cancer

JF - Genes, Chromosomes & Cancer

IS - 7

ER -