MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull

Lasse Saaby; Hasse Bonde Rasmussen; Anna Katharina Jäger

doi:10.1016/j.jep.2008.10.012

MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull

Lasse Saaby, Hasse Bonde Rasmussen, Anna Katharina Jäger

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

80 Citationer (Scopus)

Abstract

AIM OF THE STUDY: This study investigated MAO-A inhibitory activity of methanol extract of Calluna vulgaris (L.) Hull., which traditionally has been used as a nerve calming remedy. MATERIALS AND METHODS: A methanolic extract of Calluna vulgaris was partitioned against heptane, ethyl acetate and water. The three fractions were tested in a photometric peroxidase linked MAO-A bioassay. The ethyl acetate phase showed the highest MAO-A inhibitory activity. Quercetin was isolated by VLC through bioassay-guided fractionation and purified by re-crystallisation. The structure was elucidated by LC-MS and (1)H NMR. RESULTS: The IC(50)-value for MAO-A inhibition by quercetin was 18+/-0.2muM in an assay where the IC(50)-value for MAO-A inhibition by clorgylin was 0.2+/-0.02muM. CONCLUSION: The content of quercetin in Calluna vulgaris might explain the reported nerve calming effect of the plant.

Originalsprog	Engelsk
Tidsskrift	Journal of Ethnopharmacology
Vol/bind	121
Udgave nummer	1
Sider (fra-til)	178-181
ISSN	0378-8741
DOI	https://doi.org/10.1016/j.jep.2008.10.012
Status	Udgivet - 2009

Bibliografisk note

Keywords: Calluna Vulgaris; Eriaceae; Monoamine oxidase A inhibitor; Quercetin; Traditional medicine

Emneord

Det tidligere Farmaceutiske Fakultet

Adgang til dokumentet

10.1016/j.jep.2008.10.012

Citationsformater

@article{f326fea0db1811dd9473000ea68e967b,

title = "MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull",

abstract = "AIM OF THE STUDY: This study investigated MAO-A inhibitory activity of methanol extract of Calluna vulgaris (L.) Hull., which traditionally has been used as a nerve calming remedy. MATERIALS AND METHODS: A methanolic extract of Calluna vulgaris was partitioned against heptane, ethyl acetate and water. The three fractions were tested in a photometric peroxidase linked MAO-A bioassay. The ethyl acetate phase showed the highest MAO-A inhibitory activity. Quercetin was isolated by VLC through bioassay-guided fractionation and purified by re-crystallisation. The structure was elucidated by LC-MS and (1)H NMR. RESULTS: The IC(50)-value for MAO-A inhibition by quercetin was 18+/-0.2muM in an assay where the IC(50)-value for MAO-A inhibition by clorgylin was 0.2+/-0.02muM. CONCLUSION: The content of quercetin in Calluna vulgaris might explain the reported nerve calming effect of the plant.",

keywords = "Former Faculty of Pharmaceutical Sciences",

author = "Lasse Saaby and Rasmussen, {Hasse Bonde} and J{\"a}ger, {Anna Katharina}",

note = "Keywords: Calluna Vulgaris; Eriaceae; Monoamine oxidase A inhibitor; Quercetin; Traditional medicine",

year = "2009",

doi = "10.1016/j.jep.2008.10.012",

language = "English",

volume = "121",

pages = "178--181",

journal = "Journal of Ethnopharmacology",

issn = "0378-8741",

publisher = "Elsevier Ireland Ltd",

number = "1",

}

TY - JOUR

T1 - MAO-A inhibitory activity of quercetin from Calluna vulgaris (L.) Hull

AU - Saaby, Lasse

AU - Rasmussen, Hasse Bonde

AU - Jäger, Anna Katharina

N1 - Keywords: Calluna Vulgaris; Eriaceae; Monoamine oxidase A inhibitor; Quercetin; Traditional medicine

PY - 2009

Y1 - 2009

N2 - AIM OF THE STUDY: This study investigated MAO-A inhibitory activity of methanol extract of Calluna vulgaris (L.) Hull., which traditionally has been used as a nerve calming remedy. MATERIALS AND METHODS: A methanolic extract of Calluna vulgaris was partitioned against heptane, ethyl acetate and water. The three fractions were tested in a photometric peroxidase linked MAO-A bioassay. The ethyl acetate phase showed the highest MAO-A inhibitory activity. Quercetin was isolated by VLC through bioassay-guided fractionation and purified by re-crystallisation. The structure was elucidated by LC-MS and (1)H NMR. RESULTS: The IC(50)-value for MAO-A inhibition by quercetin was 18+/-0.2muM in an assay where the IC(50)-value for MAO-A inhibition by clorgylin was 0.2+/-0.02muM. CONCLUSION: The content of quercetin in Calluna vulgaris might explain the reported nerve calming effect of the plant.

AB - AIM OF THE STUDY: This study investigated MAO-A inhibitory activity of methanol extract of Calluna vulgaris (L.) Hull., which traditionally has been used as a nerve calming remedy. MATERIALS AND METHODS: A methanolic extract of Calluna vulgaris was partitioned against heptane, ethyl acetate and water. The three fractions were tested in a photometric peroxidase linked MAO-A bioassay. The ethyl acetate phase showed the highest MAO-A inhibitory activity. Quercetin was isolated by VLC through bioassay-guided fractionation and purified by re-crystallisation. The structure was elucidated by LC-MS and (1)H NMR. RESULTS: The IC(50)-value for MAO-A inhibition by quercetin was 18+/-0.2muM in an assay where the IC(50)-value for MAO-A inhibition by clorgylin was 0.2+/-0.02muM. CONCLUSION: The content of quercetin in Calluna vulgaris might explain the reported nerve calming effect of the plant.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.jep.2008.10.012

DO - 10.1016/j.jep.2008.10.012

M3 - Journal article

C2 - 19013512

VL - 121

SP - 178

EP - 181

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

IS - 1

ER -