Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

F Kirpekar; E Nordhoff; K Kristiansen; P Roepstorff; A Lezius; S Hahner; M Karas; F Hillenkamp

Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

F Kirpekar, E Nordhoff, K Kristiansen, P Roepstorff, A Lezius, S Hahner, M Karas, F Hillenkamp

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

97 Citationer (Scopus)

Abstract

Udgivelsesdato: 1994-Sep-25

Originalsprog	Engelsk
Tidsskrift	Nucleic Acids Research
Vol/bind	22
Udgave nummer	19
Sider (fra-til)	3866-70
Antal sider	4
ISSN	0305-1048
Status	Udgivet - 1994
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA

Adgang til dokumentet

http://nar.oxfordjournals.org/cgi/content/abstract/22/19/3866

Citationsformater

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title = "Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa",

abstract = "Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.",

author = "F Kirpekar and E Nordhoff and K Kristiansen and P Roepstorff and A Lezius and S Hahner and M Karas and F Hillenkamp",

note = "Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA",

year = "1994",

language = "English",

volume = "22",

pages = "3866--70",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "19",

}

TY - JOUR

T1 - Matrix assisted laser desorption/ionization mass spectrometry of enzymatically synthesized RNA up to 150 kDa

AU - Kirpekar, F

AU - Nordhoff, E

AU - Kristiansen, K

AU - Roepstorff, P

AU - Lezius, A

AU - Hahner, S

AU - Karas, M

AU - Hillenkamp, F

N1 - Keywords: Base Sequence; DNA-Directed RNA Polymerases; Lasers; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Phosphoric Diester Hydrolases; RNA; Sequence Analysis, RNA

PY - 1994

Y1 - 1994

N2 - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

AB - Enzymatically synthesized RNA samples (in vitro transcripts) were analysed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Spectra of RNA up to 150 kDA (461 nucleotides) are shown. Polymerase generated sample heterogeneity and its contribution to mass resolution are discussed. A time course exonuclease digest of a 55 nt in vitro transcript was analyzed to investigate the performance of MALDI-MS on complex mixtures. Based on these data, the analysis by MALDI-MS of DNA sequencing reactions, produced by the action of an RNA polymerase, is discussed.

M3 - Journal article

C2 - 7524029

SN - 0305-1048

VL - 22

SP - 3866

EP - 3870

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 19

ER -