Measuring Histamine and Cytokine Release from Basophils and Mast Cells

Bettina M. Jensen; Ewa Anna Bartko; Katrine Baumann; Per Stahl Skov

doi:10.1007/978-1-0716-0696-4_21

Measuring Histamine and Cytokine Release from Basophils and Mast Cells

Bettina M. Jensen^*, Ewa Anna Bartko, Katrine Baumann, Per Stahl Skov

^*Corresponding author af dette arbejde

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › peer review

9 Citationer (Scopus)

Abstract

Basophils and mast cells are known for their capability to release both preformed and newly synthesized inflammatory mediators. In this chapter, we describe how to stimulate and detect histamine released from basophils in whole blood, purified basophils, in vitro cultured mast cells, and in situ skin mast cells (the latter by microdialysis), using either a solid phase assay or flow cytometry. We also give an example of an activation protocol for basophil and mast cell cytokine release and discuss approaches for cytokine detection.

Originalsprog	Engelsk
Titel	Basophils and Mast Cells
Forlag	Springer
Publikationsdato	7 aug. 2020
ISBN (Trykt)	9781071606957, 9781071606964
DOI	https://doi.org/10.1007/978-1-0716-0696-4_21
Status	Udgivet - 7 aug. 2020
Udgivet eksternt	Ja

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10.1007/978-1-0716-0696-4_21

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Tjek tilgængelighed hos det Kgl. Bibliotek

Citationsformater

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