Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Histology and Histopathology |
| Vol/bind | 24 |
| Udgave nummer | 5 |
| Sider (fra-til) | 573-86 |
| Antal sider | 13 |
| ISSN | 0213-3911 |
| Status | Udgivet - 2009 |
Bibliografisk note
Keywords: Adult Stem Cells; Animals; Brain Injuries; Cell Cycle Proteins; Cell Movement; Cell Proliferation; DNA-Binding Proteins; Female; Fibroblast Growth Factors; Gold; Immunohistochemistry; Inflammation; Mice; Mice, Inbred C57BL; Nerve Regeneration; Neurogenesis; Neurons; Nuclear Proteins; Vascular Endothelial Growth Factor ACitationsformater
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I: Histology and Histopathology, Bind 24, Nr. 5, 2009, s. 573-86.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Metallic gold treatment reduces proliferation of inflammatory cells, increases expression of VEGF and FGF, and stimulates cell proliferation in the subventricular zone following experimental traumatic brain injury
AU - Pedersen, Mie Østergaard
AU - Larsen, Agnete
AU - Pedersen, Dan Sonne
AU - Stoltenberg, Meredin
AU - Penkowa, Milena
N1 - Keywords: Adult Stem Cells; Animals; Brain Injuries; Cell Cycle Proteins; Cell Movement; Cell Proliferation; DNA-Binding Proteins; Female; Fibroblast Growth Factors; Gold; Immunohistochemistry; Inflammation; Mice; Mice, Inbred C57BL; Nerve Regeneration; Neurogenesis; Neurons; Nuclear Proteins; Vascular Endothelial Growth Factor A
PY - 2009
Y1 - 2009
N2 - Traumatic brain injury represents a leading cause of morbidity in young individuals and there is an imperative need for neuroprotective treatments limiting the neurologic impairment following such injury. It has recently been demonstrated that bio-liberated gold ions liberated from small metallic gold implants reduce inflammation and neuronal apoptosis, while generating an increased neuronal stem cell response following focal brain damage. In this study mice were subjected to a unilateral traumatic cryo-lesion with concomitant injection of 25-45 microm gold particles near the lesion. Placebo-treated mice subjected to cryo-lesion served as controls. The effects of gold-treatment were investigated by examining gold-induced growth factor expression (VEGF and FGF) in the first two weeks after the insult, and the extent of the neurostimulatory effect of gold was explored by comparing cell proliferation in the subventricular zone as judged by immunohistochemical staining for CDC47. Vimentin staining revealed a decrease in activated microglia and a transient astrogliosis in response to the gold liberation. Moreover, gold ions significantly increase the expression of VEGF and FGF following trauma and a significant increase in cell proliferation in both the ipsilateral and the contralateral subventricular zone was found in response to gold-treatment. In conclusion: we confirmed the previously demonstrated anti-inflammatory effect of bio-liberated gold ions, and further show that metallic gold increases growth factor expression and adult neurogenesis.
AB - Traumatic brain injury represents a leading cause of morbidity in young individuals and there is an imperative need for neuroprotective treatments limiting the neurologic impairment following such injury. It has recently been demonstrated that bio-liberated gold ions liberated from small metallic gold implants reduce inflammation and neuronal apoptosis, while generating an increased neuronal stem cell response following focal brain damage. In this study mice were subjected to a unilateral traumatic cryo-lesion with concomitant injection of 25-45 microm gold particles near the lesion. Placebo-treated mice subjected to cryo-lesion served as controls. The effects of gold-treatment were investigated by examining gold-induced growth factor expression (VEGF and FGF) in the first two weeks after the insult, and the extent of the neurostimulatory effect of gold was explored by comparing cell proliferation in the subventricular zone as judged by immunohistochemical staining for CDC47. Vimentin staining revealed a decrease in activated microglia and a transient astrogliosis in response to the gold liberation. Moreover, gold ions significantly increase the expression of VEGF and FGF following trauma and a significant increase in cell proliferation in both the ipsilateral and the contralateral subventricular zone was found in response to gold-treatment. In conclusion: we confirmed the previously demonstrated anti-inflammatory effect of bio-liberated gold ions, and further show that metallic gold increases growth factor expression and adult neurogenesis.
M3 - Journal article
C2 - 19283666
SN - 0213-3911
VL - 24
SP - 573
EP - 586
JO - Histology and Histopathology
JF - Histology and Histopathology
IS - 5
ER -